With regards to reverse transcriptase, what does reverse transcriptase copy number mean

Upstairs, don't mistake people, virus-infected cells only nucleic acids enter the cell, the shell will not enter, reverse transcriptase genes are only a small part of the virus genes, there are many coding shell proteins, etc., the space in the virus shell is very small and there will be no room for reverse transcriptase, so only nucleic acids enter, viruses are divided into positive DNA, negative DNA, positive RNA, negative RNA, double-stranded DNA, double-stranded RNA, double-stranded DNA, double-stranded DNA, and I recommend you to review the transcription and translation process of biochemistry and molecular biology. Foreign "Principles of Virology", don't tell me that my noun is wrong, the translation is probably that and I am a medical professional.

Only the positive strands of double-stranded DNA can transcribe mRNA because of the limitation of enzymes in the nucleus, and normal eukaryotic cells do not have reverse transcriptase, but some people say that 8% of the human genome is a viral gene gene

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There are two ways in which reverse transcriptases are produced.

The coat protein of the virus contains reverse transcriptase, so the reverse transcriptase after RNA invades the cell is brought into the cell by the virus itself.

2.Some RNA viruses have RNA that can encode reverse transcriptase.

Summary. Reverse transcriptase (ReverseTranscriptase) is also known as RNA-guided DNA polymerase. It is an enzyme that uses RNA as a template to synthesize DNA.

This enzyme was discovered in 1970 by the American scientists Temin (and Baltimore) in animal carcinogenic RNA viruses, for which they were awarded the 1975 Nobel Prize in Physiology or Medicine. When an RNA oncogenic virus, such as Rous sar-coma virus in birds, enters the host cell, its reverse transcriptase first catalyzes the synthesis of a single strand of DNA complementary to the viral RNA, and then replicates the double-helix DNA, which is integrated into the host's chromosomal DNA by the action of another viral enzyme. Some of it serves as the genetic material of the virus, and the other part is translated into virus-specific proteins as mRNA. Finally, RNA and proteins are assembled into new virions.

Under certain conditions, integrated DNA can also enable cells to transform into cancer cells.

Hello, I am inquiring for you, please wait a while, I will reply to you immediately Hello, I am happy to answer for you: reverse transcriptase uses viral RNA as a template to synthesize DNA from raw materials in host cells.

Reverse transcriptase (ReverseTranscriptase) is also known as RNA-guided DNA polymerase. It is an enzyme that uses RNA as a template to synthesize DNA. This enzyme was discovered in 1970 by American scientists Temin (Shengai) and Baltimore (respectively in animal carcinogenic RNA viruses, for which they won the 1975 Nobel Prize in Physiology or Medicine.

When an RNA oncogenic virus, such as Rous sar-coma virus in birds, enters the host cell, its reverse transcriptase first catalyzes the synthesis of a single strand of DNA complementary to the viral RNA, and then replicates the double-helix DNA, which is integrated into the host's chromosomal DNA by the action of another viral enzyme. Some of it serves as the genetic material of the virus, and the other part is translated into virus-specific proteins as mRNA. Finally, RNA and proteins are assembled into new virions. Under certain conditions, integrated DNA can also enable cells to transform into cancer cells.

The process of reverse transcription is catalyzed by reverse transcriptase. This reverse transcriptase, also known as RNA-dependent DNA polymerase (RDDP), is a synthetic simple fiber that uses RNA as a template to catalyze the DNA strand. The synthetic DNA strand is called ComplementaryDNA (cDNA) with RNA.

Reverse transcriptase is present in some RNA viruses and may be involved in the malignant transformation of the virus.

The human immunodeficiency virus (HIV) is also an RNA virus that contains reverse transcriptase. Reverse transcriptase is also found in normal cells and embryonic cells of mice and humans, and it is speculated that it may be related to cell differentiation and embryonic development.

The role of reverse transcriptase is to use DNTP as the substrate, RNA as the template, and tRNA (mainly tryptophan tRNA) as the primer, in tRNA3'- On the end, press 5'→3'direction, synthesizing a single strand of cDNA that is complementary to the RNA template, which forms an RNA-cDNA hybrid. Subsequently, the RNA strand was hydrolyzed under the action of reverse transcriptase, and the second DNA strand was synthesized using cDNA as a template. At this point, the RNA synthesis process guided by RNA is completed.

The three roles of reverse transcriptase:

Anti-excitation 1, RNA as template-guided DNA polygenase activity;

2. DNA as template-guided DNA polymerase activity;

3. RNaseh activity.

RNA-directed DNA polymerase activity; Using RNA as a template, catalyze the process of polymerization of DNTP into DNA. This enzyme requires RNA as primers, mostly tryptophan tRNA, so it has no correction function, so the error rate of DNA catalyzed by reverse transcriptase is relatively high.

rnaseh activity; The hybrid molecule formed by the synthesis of cDNA catalyzed by reverse transcriptase with template RNA will hydrolyze the RNA molecule from the 5th end of the RNA by RNAseh.

DNA-directed DNA polymerase activity; Using the first single strand of DNA synthesized by reverse transcription as a template, Li Lushen used DNTP as a substrate to synthesize the second DNA molecule.