What is the regular disinfection of the clean room? 10

Regarding the precautions for the operation of the food aseptic purification workshop, please refer to the following: 1. Strict aseptic operation to prevent microbial contamination; Operators should turn off the UV lamp before entering the sterile room. 2. The food aseptic workshop should be equipped with an aseptic operation room and a buffer room, and the cleanliness of the aseptic operation room should reach 10,000 levels, the indoor temperature should be kept at 20-24, and the humidity should be kept at 45-60%.

The cleanliness of the ultra-clean table should reach 100 grades. 3. The sterile room should be kept clean, and it is strictly forbidden to pile up sundries to prevent pollution. Strictly prevent the contamination of all sterilization equipment and culture media, and stop using those that have been contaminated.

4. The sterile room should be sterilized and cleaned regularly with appropriate disinfectant to ensure that the cleanliness of the sterile room meets the requirements. 5. All items such as instruments, instruments, and plates that need to be brought into the sterile room should be tightly bandaged and sterilized by appropriate methods. 6. Before entering the sterile room, the staff must wash their hands with soap or disinfectant, and then change into special work clothes, shoes, hats, masks and gloves in the buffer room (or wipe their hands again with 70% ethanol) before entering the sterile room for operation.

7. Before the use of the clean room, the ultraviolet lamp of the clean room must be turned on for irradiation sterilization for more than 30 minutes, and the ultra-clean table must be opened at the same time for blowing. After the operation, the sterile room should be cleaned in time and sterilized with ultraviolet lamp irradiation for 20 minutes. 8. Before the inspection, the outer packaging of the test product should be kept intact and should not be opened to prevent contamination.

Before the examination, disinfect the outer surface with a 70% alcohol cotton ball. 9. All items with live bacteria must be disinfected before they can be flushed under the faucet, and it is strictly forbidden to pollute the sewer.

The disinfection and sterilization of the clean room can be carried out in the following ways:

l Fumigation with mixed formaldehyde and potassium permanganate: generally 10 ml of 40 formaldehyde and 8 ml of potassium permanganate per square meter are required for fumigation. When using, first close the doors and windows, measure the formaldehyde solution into the container, and then pour in a good amount of potassium permanganate, and then leave the inoculation room, close the door, and fumigate for 20-30 minutes.

2 liters of mercury water disinfection: use gauze or sponge soaked in mercury water for finger wiping, or spray sterilization with a sprayer, so that the top and bottom of the box are stained with mercury water, hands can also be disinfected with mercury water, and the sleeves are rolled up. 20 to 30 minutes after spraying, the bacteria in the box and the droplets fall to the bottom of the box to be killed, and the air inside becomes very clean.

3. Ultraviolet irradiation sterilization: Install a 200 volt, 3o watt ultraviolet lamp in the sterile box; Open for 20 to 30 minutes each time, you can achieve the purpose of space sterilization. After the irradiation is over, cover with a black cloth for half an hour to enhance the sterilization effect.

4 Carbolic acid spray: Before each inoculation, spray with 5 carbolic acid solution can promote the sedimentation of particles and miscellaneous bacteria in the air, prevent the dust on the table from flying, and have a bactericidal effect.

5 Lime rubbing: often fumigated with drugs, easy to cause an acidic environment, especially with formaldehyde and potassium permanganate fumigation for a long time, pollution is often more and more serious, the prevention method can be a variety of drugs alternately, after a period of time (about five weeks) with lime scrubbing. This has proven to work well.

Sterilization in the clean room can be carried out in the following three ways: fumigation, spray and ultraviolet irradiation.

1) Fumigation: This is a measure of thorough sterilization in the chamber without loss. The sterile room has been used for a long time, and fumigation sterilization should be carried out when the sensitive contamination is serious. It can be fumigated with formaldehyde, lactic acid or sulfur.

2) Spray: Performed before each use of the sterile chamber. The spray can promote the sedimentation of particles and microorganisms in the air, prevent the dust on the desktop and the ground from flying, and have a sterilization effect. Can be sprayed with 5% carbolic acid.

3) Ultraviolet irradiation: Performed before each use of the clean room. Ultraviolet rays have a good sterilization effect. Usually the UV lamp should be turned on for 30 to 60 min.

Answer]: b dry baking method is mainly used for side disinfection of glassware, porcelain, glass syringes, etc.; Ultraviolet irradiation can only be used to disinfect surfaces and disinfection of air and heat-resistant surfaces in operating rooms, sterile operation rooms, burn wards; The autoclave sterilization method is suitable for objects that are resistant to high temperature and high rolling pressure and are not afraid of moisture; Yunhuqiao pasteurization method is mainly used for milk and other disinfection; The filtration and sterilization method can be used for indoor air disinfection and disinfection of some serum, toxins, antibiotics and other products that are not resistant to high temperature sterilization.

Dear, I'm honored to have your question The information that the teacher found for you here is: What is used for greenhouse air sterilization and disinfection: 1

The disinfection of modern large-scale multi-story greenhouses is often carried out in the summer high temperature season. One is to use sulfur or smoke agent fumigation and disinfectionThe second is the use of steam sterilization.

The high-pressure steam is introduced into the soil, and the high temperature is used to kill the germs and insect eggs in the soil, and the effect is better. 2.The north side of the single roof is a greenhouse with earthen walls, because it is a temporary building, it can be rebuilt in another place to avoid the damage of pests and diseases caused by continuous cropping.

3.For the disinfection of the stationary single-roof heating greenhouse, it can be fumigated and disinfected with sulfur sawdust and chlorothalonil fog agent about 1 week before sowing seedlings or planting.

1. The environment should be clean, and half an hour before aseptic operation, the cleaning of the ground and other work must be stopped. Avoid unnecessary crowd movements and prevent dust from flying. **The room should be disinfected with ultraviolet light once a day.

2. When performing aseptic operation, the sterile and non-sterile areas of the article must be clarified.

3. Before performing aseptic operation, wear a hat and mask, wash your hands, dry your hands, and pay attention to air and environmental cleanliness.

4. To clamp sterile items, sterile forceps must be used. 4. When performing aseptic operation, all unsterilized hands and arms should not directly touch sterile items or take objects beyond the sterile area.

5. Sterile items must be kept in a sterile bag or sterilization container, and should not be exposed to air for too long. Sterile and non-sterile materials should be placed separately. Once opened, a sterile bag cannot be considered absolutely sterile and should be used as soon as possible.

Sterile items that have been removed cannot be put back into the sterile container even if they have not been used.

6. The sterile bag should be placed in the fixed cabinet in the order of the disinfection date, and kept clean and dry, separated from the non-sterilized bag, and frequently check whether the sterile bag or container is expired and whether the materials used in it are appropriate.

7. Sterile saline and alcohol, Xinjieer cotton ball tank should be disinfected once a week, and the dressings in the container, such as dry cotton balls, gauze blocks, etc., should not be overfilled, so as not to be contaminated outside the container when taking it.

Air sterilization in the clean room is fumigation with a mixture of formaldehyde and potassium permanganate.

1. Generally, 10 ml of 40 formaldehyde and 8 ml of potassium permanganate are required per square meter for fumigation.

2. When using, close the doors and windows first, measure the formaldehyde solution into the container, and then pour in a good amount of potassium permanganate, and then leave the inoculation room, close the door, and fumigate for 20-30 minutes.

What I said downstairs is what I want to say. In the tank. I'm also doing purification works here. Welcome.

Summary. Hello, dear. The sterilization methods that can be used in the clean room are the use of ultraviolet ozone sterilization in the environment, the disinfection of surfaces with disinfectants such as alcohol, and the use of high-temperature sterilization or autoclaving of utensils.

Kiss, hello and missing. The sterilization method that can be used in the clean room is to make the environment empty, use ozone sterilization outside the purple sparrow, disinfect the surface with alcohol and other disinfectants, and use high temperature sterilization or autoclaving of utensils.

Hello, dear. The sterile room is generally a small room of 4-5 square meters and a height of 4 to 5 meters (isolated from the outside), which is specially located in the microbiology laboratory and can be built with plates and glass. A buffer room should be set up outside the sterile room, and the door should be staggered to prevent the airflow from bringing in bacteria.

The clean room and buffer room must be closed, and the ventilation equipment equipped in the room must have an air filtration device. After obtaining a sterile environment and sterile materials, only by maintaining sterility can Shenzhou conduct research on a specific known microorganism.