What are the media for filamentous fungi?

1.Divide according to the composition of the medium.

According to the components it contains, culture media can be divided into three categories: synthetic medium, natural medium and semi-synthetic medium.

1) Synthetic medium. The various components of synthetic media are completely known various chemicals. The chemistry of this medium is clear, the composition is precise, and the reproducibility is strong, but it is more expensive, and microorganisms grow slower in this type of medium.

Such as Gao's No. 1 synthetic medium, Cha's (Czapek) medium, etc.

2) Natural medium. Made from natural substances such as steamed potatoes and regular beef broth, the former is used to grow mold and the latter is used to grow bacteria. The chemical composition of these media is very unconstant and difficult to determine, but they are easy to prepare and nutritious, so they are often used.

3) Semi-synthetic medium. Inorganic salts of known composition are appropriately added on the basis of natural organic matter, or certain natural components are added on the basis of synthetic medium, such as potato glucose agar medium for cultivating molds. These media are more effective at meeting the nutrient needs of microorganisms.

2.According to the physical state of the medium.

According to its physical state, culture media can be divided into three categories: solid medium, liquid medium and semi-solid medium.

1) Solid medium. It is to add coagulants to the medium, including agar, gelatin, silica gel, etc. Solid media are commonly used for microbial isolation, identification, enumeration, and culture preservation.

2) Liquid medium. No coagulant is added to the liquid medium. The composition of this medium is uniform, and the microorganisms can fully contact and utilize the nutrients in the medium, which is suitable for physiological research, and is also commonly used in the fermentation industry because of the high fermentation rate and easy operation.

3) Semi-solid medium. It is a semi-solid state when a small amount of coagulant is added to the liquid medium. It can be used to observe the movement of bacteria, identify bacterial species, and determine the potency of bacteriophages.

3.It is divided according to the type of microorganisms.

According to the type of microorganisms, the culture medium can be divided into four categories: bacterial culture medium, actinomycete culture medium, yeast culture medium and mold culture medium.

Commonly used bacterial culture media are nutrient broth and nutrient agar medium; The commonly used actinomycete medium is Gao's No. 1 medium; Commonly used yeast media are potato sucrose medium and wort medium; Commonly used mold culture media include potato sucrose medium, bean sprout juice glucose (or sucrose) agar medium, and Cha's medium.

4.According to the purpose of the medium.

According to its special purpose, culture media can be divided into enrichment medium, selective medium and discrimination medium.

1) Enrichment medium. It is to add blood, serum, animal and plant tissue extracts to the medium to cultivate some microorganisms with more demanding requirements.

2) Selective medium. It is a medium designed according to the special nutritional requirements of a certain type or a certain class of microorganisms or some physical and chemical resistance. This medium can be used to isolate the desired microorganisms from the mixed microorganisms.

3) Identification medium. It is to add a certain reagent or chemical to the culture medium, so that a certain change will occur after culture, so as to distinguish different types of microorganisms.

Culture medium refers to the nutrient matrix prepared by the combination of different nutrients for the growth and reproduction of microorganisms, plants or animals (or tissues), generally containing carbohydrates, nitrogenous substances, inorganic salts (including trace elements), vitamins and water and other major categories of substances The medium is not only the basic substance that provides cell nutrition and promotes cell proliferation, but also the living environment for cell growth and reproduction According to the physical state, it can be divided into solid medium, liquid medium, semi-solid medium, according to the culture function, it can be divided into basic medium, selection medium, enrichment medium, identification medium, etc. According to the scope of use, it can be divided into bacterial medium, actinomycete medium, yeast medium, fungal medium, etc.

Commonly used media for bacterial culture are:

1.Beef liquid (meat water) is used as a base fluid for the preparation of various culture media.

2.Liquid broth (common broth) is used for general bacterial culture.

3.Nutrient agar medium (ordinary agar) is used for general bacterial isolation and culture, pure culture, observation of colony traits and strain preservation, and the basis of special medium.

4.Semi-solid medium works the same as ordinary agar.

5.Fresh blood agar is used for isolation culture and culture preservation.

Bacteria can be cultivated anywhere, but the medium provides sufficient nutrients and the necessary factors for bacteria to grow.

The general steps for culturing bacteria and fungi are as follows:

1. Prepare tools and materials: petri dishes, sterile cotton swabs, pressure cookers, constant temperature incubators, sterile workbenches, beef juices, agar.

2. Configure nutrient base: choose nutrients such as beef juice and mix them with agar.

3. High temperature early sterilization: the configured nutrient base is sterilized at high temperature to kill the original bacteria and fungi in the medium.

4. Cooling inoculation: transfer a small amount of bacteria or fungi to the medium, and do a good job of disinfection and sterilization of the environment and instruments to prevent miscellaneous bacterial infection.

5. Constant temperature culture: put the inoculated petri dish in the incubator for culture.

6. Bacteria (scientific name: bacteria) is one of the main groups of organisms [1], belonging to the bacterial domain and the kingdom of bacteria, and it is also the most abundant class of all organisms. It comes in a variety of shapes, including spherical, rod-shaped, and spiral-shaped.

7. Bacteria have a great impact on human activities. On the one hand, bacteria are the causative agents of many diseases, including tuberculosis, gonorrhea, anthrax, syphilis, plague, trachoma and other diseases. However, bacteria are also often used by humans, such as the production of cheese and yogurt and wine, the manufacture of some antibiotics, and the treatment of wastewater.

8. Cook some broth, add a little salt, and put the agar into it while it is hot, and so it is solidified, the medium is done, the key is to inoculate, how to cultivate without pure strains, if the requirements are not strict, cover the lid, put it in the place where it is about 25 degrees, and put it for 24 to 48 hours, you can see the colony, which is miscellaneous.

Sachs' glucose agar medium is also known as SDA medium.

Shahs's medium, is a general-purpose fungal medium, belongs to a kind of nutrient medium, Shahshir glucose agar medium belongs to a modified Shahsler agar, mainly used to cultivate and distinguish fungi, and this medium is generally used with antibiotics, by using Shahser glucose agar medium can be used to isolate pathogens and fungi in bacterial materials. Sassen's glucose agar medium is mainly used to identify and cultivate fungi, especially those fungi. Some will also be used for the identification of fungi in sedimentation bacteria and planktonic fungi in the clean area of the pharmaceutical industry.

The high sugar content and low pH value of Sashar's glucose agar medium are very conducive to the growth of fungi and can effectively inhibit the growth of bacteria. Mycological peptones provide sources of nitrogen, sulfur, minerals and vitamins. Fungi grown in Sarson's glucose agar medium can retain their typical morphological characteristics, so that the species can be identified by different morphological characteristics.

Potato glucose agar medium for short, PDA is used to culture fungi, wort medium is used to culture yeasts and filamentous fungi.

Martin's medium is used to screen soil fungi.

Caramelization medium Yeast enrichment medium For yeast cultivation In most cases, PDA medium is available in the laboratory unless you want to grow some specific species.

There are a lot of professional books on this subject, and I recommend you to go and buy one online.

The fungus should be cultured with modified Martin's medium.

Modified Martin's medium is a very widely used in vitro diagnostic reagent in medical experiments and biological research, and the modified Martin's medium is mainly used in the liquid medium for fungal growth in blood, chest, ascites and other specimens to detect whether there is fungi in the specimens, so as to judge whether the tissue is infected by fungi.

The main ingredients are:

Fish peptone, glucose, yeast powder, dipotassium phosphate trihydrate, magnesium sulfate heptahydrate, sodium chloride, distilled water.

Answer] :d analysis: Commonly used for fungal culture medium, containing antibiotics and actinomycetes (inhibiting bacteria, actinomycetes sung long).

Chocolate medium: meningococcal; Eosin-Melin medium: identification of E. coli; Potassium tellurate medium:

diphtheria bacillus; Blister meat culture slow-trophic base: anaerobic bacteria. Master the knowledge of "fungi".