Is it possible to extract paclitaxel personally, does anyone know, thank you 5

Personally, I think it's unlikely, because:1Yew (all species) is a nationally protected plant, which means you can't get a lot of raw material from formal sources; 2.

Paclitaxel extraction is complex, involves many steps, and the process is complex, and it is not something that can be done by one person alone. Attached is the extraction method, which originates from the network.

A method for efficient separation and purification of paclitaxel.

Separation steps. It includes a. extraction, which uses yew as raw material to obtain an extract containing paclitaxel; b. Remove the gum and remove the gum impurities in the extract; c. Separation and purification.

Process characteristics. Yew bark crushed (the finer the better), 85 95 alcohol 35 55 hot reflux extraction three times, 50 70 vacuum decompression concentration to thermal specific gravity, chloroform extraction, the extract was concentrated into a paste, paclitaxel content 1 chloroform paste was dissolved, the paclitaxel content 1 chloroform paste plus chloroform was completely dissolved, silica gel was added and stirred evenly, cooled and dried, sieved, filled into the chromatography column, chloroform methanol gradient elution, TLC detection, segmented merge and concentration, paclitaxel content 5 8 semi-finished products, paclitaxel content 5 8 The semi-finished product is completely dissolved with acetone, stirred evenly with silica gel, cooled and dried, sieved, and filled into the chromatography column, acetone petroleum ether gradient elution, TLC detection, segmented merging and concentration, and the paclitaxel content is obtained 20 25 semi-finished products, crystallized with acetone petroleum ether system 3 4 times, suction filtration, 50 vacuum decompression drying, paclitaxel content 75 80 semi-finished products, 16MPa pressure chromatography separation, TLC detection, segmented merge concentration, target section concentrate acetone petroleum ether crystallization, suction filtration, drying, and paclitaxel content 99 5 finished products;

The process of removing gum is as follows:

High-pressure silica gel chromatography column chromatography was used to remove the colloids, and at the same time, the taxane compounds were separated into three parts: paclitaxel, harringtaxine, and 7-table paclitaxel.

I have read a lot of domestic information about the extraction of paclitaxel**, because the authors of these articles are not clear about the anti-cancer mechanism of paclitaxel, although they have made many long comments, but they cannot mention paclitaxel with anti-cancer activity, which wastes yew resources. If you copy the practice of domestic experts, you will definitely not be able to mention paclitaxel with anti-cancer activity. Like the above article is blind toe, the paclitaxel proposed in this way does not have anti-cancer activity at all.

Here's how to <>:

1. The method of extracting paclitaxel from natural product yew mainly uses the bark of yew to extract paclitaxel;

3. Semi-artificial synthesis refers to the method of obtaining the derivatives of paclitaxel from the branches and leaves of yew and obtaining paclitaxel through chemical synthesis and modification;

4. Biosynthesis of paclitaxel, paclitaxel is mainly composed of taxane rings and side chains, which is of great significance for artificially oriented to improve synthesis efficiency and cloning combination, form key enzyme genes, and improve the yield of paclitaxel;

5. Chemical synthesis of paclitaxel, according to research and reports, the activity of deacetylberry gibberellin isolated from yew plants is lower than that of paclitaxel, but it can be extracted from yew needles, and the substance can be synthesized into paclitaxel through a four-step chemical process;

6. Microbial synthesis of paclitaxel, a parasitic fungus is isolated from the phloem part of Taxus brevifolia, which can produce paclitaxel and related hydrocarbons in a specific medium;

7. Recombinant DNA technology is expected to increase the yield of paclitaxel, and the use of Agrobacterium rhizoids to induce rooting of yew plant explants, and the binding of key enzymes and cloned yew related genes from the microbial synthesis pathway can reduce the dependence of paclitaxel synthesis on yew.

1. Paclitaxel is indeed a drug that has an effect on inhibiting cancer, but it is not a panacea, and at present, it can only control cancer, and cannot ** cancer. Generally, paclitaxel preparations are used in combination with chemotherapy.

Second, there are yew trees near your house, but they are all wild and owned by the state. Yew is a national second-class protected plant, and unauthorized logging is an illegal act, which is subject to fines and criminal responsibility. If there is a research institution that wants to study yew, it also needs to apply to the ** department, and it is not that you can provide it if you say it.

3. The content of paclitaxel in yew is very low, even if you cut and refine the yew around your home, you may only get dozens of grams of paclitaxel, which is not enough for cancer, not to mention the high cost of extracting paclitaxel. Although paclitaxel is known as the last line of defense for human beings against cancer, in fact, there are still many problems that need to be solved in practical application, which is why hospitals do not use paclitaxel to ** cancer.

4. Yew is recognized as an endangered natural and rare anti-cancer plant in the world, and paclitaxel extracted from the bark and leaves of yew has a high curative effect on a variety of late-stage cancers, and is known as "the last line of defense for cancer". With the continuous development of supercritical fluid extraction technology, it will bring a broader space for the extraction and purification of paclitaxel.

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Paclitaxel is the main active ingredient in Taxus chinensis and is a natural secondary metabolic derivative of terpenoid cyclic structure. Due to its low content, many structural analogues, high purity requirements, and easy conversion during separation, it has brought great difficulties to extraction, separation and determination. How to extract paclitaxel in a low-cost and simple process, separate paclitaxel from its analogues, obtain paclitaxel products with high purity, and accurately determine them has become the focus of research.

Commonly used extraction methods include: methanol organic solvent extraction, ethanol organic solvent extraction, etc. Extraction can be divided into primary extraction and secondary extraction, and in these two stages of extraction, the choice of solvent has an important impact.

Commonly used purification methods include: normal-phase column chromatography and reversed-phase chromatography mainly include atmospheric and low-pressure column chromatography, dry column chromatography, etc., as well as some more advanced separation methods such as high-speed countercurrent chromatography.

At present, most of the extraction methods of paclitaxel substances use organic solvents to extract yew raw materials for many times, and then extract with organic solvents, and then crystallize to obtain paclitaxel after chromatography separation. The biggest problem of these methods is that the extraction yield is low and the equipment is expensive, and it causes a huge waste of resources, increases the production cost, and has great environmental problems.

The specific extraction steps are as follows: take the yew raw material and grind it through a 100 to 200 mesh sieve, add 5 to 10 times the amount of water and 0.1% glacial acetic acid to it, and add cellulase to enzymatic hydrolysis under ultrasound for 2 to 5 hours; Equal volumes of organic solvent were added and ultrasonically extracted for 2 hours; Static filtration separation, aqueous phase with organic solvent extraction 2 to 3 times, combined organic phase; The organic solvent was removed under reduced pressure, the obtained extract was mixed with neutral alumina of the same quality, and the fat-soluble impurities were eluted by petroleum ether and ethyl acetate through a 200 to 300 mesh silica gel or alumina chromatography column, and then eluted with a mixture of alcohol and ethyl acetate, and the fluids of alcohol and ethyl acetate were monitored. The alcohol and ethyl acetate were removed under reduced pressure, and the crude product obtained was eluted by a weak alkaline resin column and a strong alkaline resin column, and a mixture of dichloromethane and acetone to obtain a pure product. The pure product is then recrystallized and refined.

You can't mention it yourself, the domesticated kind can't do it, that's what I'm doing. Yew releases oxygen for 24 hours and purifies the air. There are extremely high requirements for breeding technology and land.

To more than 10,000 acres of land, a large amount of capital injection is required, and the extracted paclitaxel has a high rate of some cancers, especially cancers. The 2nd extract is Sequoia Pure has an excellent ** effect on diabetes. National first-class CDMA protected plants.

The fruit is poisonous. Unscrupulous businesses on the Internet don't recommend using yew cups and the like, they don't have the technology to completely remove toxins.

JD.com** directly buys totem bf839 oral liquid. It is used for monthly consumption of 1,680 yuan for health care. If used for**Preferably 2 or 3.

It is only known that paclitaxel is useful for breast cancer, non-small cell lung cancer, rectal cancer, and one type of cancer. It is not clear whether it has a role in liver cancer.

You don't know, do you? One kilogram of high-purity paclitaxel costs 2 million yuan, because this extract is difficult to extract, 10 acres of yew trees can extract one kilogram of %1 purity paclitaxel every year. Moreover, it is a new type of anti-cancer drug that has good efficacy on a variety of cancers and is smaller after doxorubicin and cisplatin.

Paclitaxel is used for ovarian and breast cancer and is the first FDA-approved chemical drug from natural plants. It was listed in 1993 by BMS Corporation.

Chinese alias: paclitaxel.

English name: paclitaxel

CAS accession number: 33069-62-4

MF: C47H51NO14

Molecular weight: Pharmacological efficacy: anti-tumor, efficacy is mainly anti-tumor, and there are many other taxanes: 10-deacetylbaccatin III, 7-epi-10-deacetylpaclitaxel, 10-deacetylpaclitaxel, 7-epitaxel, harringine.

First- and second-line ovarian and breast cancers and NSCLC, head and neck cancer, esophageal cancer, seminoma, and non-Hokin's lymphoma, all I know.

Here's how to <>:

1. The method of extracting paclitaxel from natural product yew mainly uses the bark of yew to extract paclitaxel;

3. Semi-artificial shielding synthesis refers to the method of obtaining the derivatives of paclitaxel from the branches and leaves of yew and obtaining paclitaxel through chemical synthesis and modification;

4. Biosynthesis of paclitaxel, paclitaxel is mainly composed of taxane rings and side chains, which is of great significance for artificially oriented to improve synthesis efficiency and cloning combination, form key enzyme genes, and improve the yield of paclitaxel;

5. Chemical synthesis of paclitaxel, according to research and reports, the deacetylberry gibberellin isolated from yew plants is lower than that of paclitaxel, but it can be extracted from yew needles, and the substance can be synthesized into paclitaxel through a four-step chemical process;

6. Microbial synthesis of paclitaxel, a parasitic fungus is isolated from the phloem part of Taxus brevifolia, which can produce paclitaxel and related hydrocarbons in a specific medium;

7. Recombinant DNA technology is expected to increase the yield of paclitaxel, and the use of Agrobacterium rhizoids soaked in Taxus plant explants to induce macro-collapse bridge roots, from the microbial synthesis pathway, the key enzymes are directed to the clonal Taxus related genes combined, which can reduce the dependence of paclitaxel synthesis on Taxus.