What is the reason for the heating up of the cryostat when it is turned on

Freeze microtome temperature setting, turn on the power 2 hours before use, and set the temperature to minus 25.

Place the specimen of the appropriate size and thickness on a mushroom-shaped cup, add frozen wax, clamp it in the fixation tank of the microtome, prepare the slide, select the thickness of the slide, shake the handle, and the section is made.

Carefully adsorb the slices on the glass sheet, turn off the power after use, clean the cavity and dry the water vapor, and make a record of the use to allow water vapor.

To evaporate, do not close the lid sliding door of the microtome immediately.

It should be closed the next day.

The switch on the right turns on the power supply, and the temperature setting button of the box is used to set the temperature of the microtome to the desired temperature of the slicing, and the temperature display window displays the set temperature when the temperature is set, and the actual temperature is displayed after the setting stops for 5 seconds.

The temperature of the freezing table of the frozen microtome is the temperature of the tissue freezing, and the temperature of the box and the temperature of the tissue chuck have a great influence on the temperature of the section, so the temperature setting of the frozen microtome is recommended, the temperature of the quick-freezing table should be lower than minus 30, and the temperature of the box and the tissue chuck should be set at minus 16 to minus 20.

Cryostats and when cutting biological tissues, tissue samples need to be frozen to very low temperatures in order to preserve their morphology and structure. However, if the cryostat melts as soon as it is sliced, it may be caused by the following reasons:

1.The temperature of the microtome is not low enough: If the temperature of the microtome is not low enough, the tissue sample will gradually melt during the cutting process, resulting in incomplete or complete melting of the section.

2.Poor tissue sample quality: If the tissue sample is of poor quality, such as high water content, uneven freezing, etc., it will also cause the tissue sample to melt during sectioning.

3.The microtome blade is not sharp enough: If the microtome's blade is not sharp enough, it can cause the tissue sample to be squeezed and rubbed during the cutting process, which can melt.

4.Improper use of the microtome: If the user does not follow the correct operating procedures, such as too fast slicing speed and uneven slice thickness, it can also cause the tissue sample to melt.

Therefore, if everything is resolved in the cryostat, it is necessary to check the above problems, find out the problems and adjust and deal with them accordingly.

When a cryostat cuts food, it needs to freeze the food so that it is easier to cut. However, if the slicer's knife or slicing process is not right, it can lead to problems with food cutting, such as loose ingredients or excessive moisture. If these problems occur, the food may begin to melt after cutting, resulting in a lump of the entire item"Melt away", which is usually caused by the high moisture content of the food produced during the cutting process.

To avoid this, consider freezing the food again for a period of time to allow the moisture to condense and cure before using a slicer to cut it. In addition, before using the slicer, you should also make sure that it is functioning properly and choose the appropriate cutting method and parameters according to different types of food products.