Step to place the bioload under a microscope for observation

1. Take out the microscope, observe the eyepiece, and adjust the light source (or mirror).

2. Place the bioload on the stage of the microscope and fix it.

3. Side-looking microscope, lower the lowest magnification objective to the nearest to the biological mounting.

4. Observe the eyepiece and gradually raise the eyepiece until the image is clear (first adjust the coarse quasi-banana spiral, and then adjust the fine quasi-focal spiral).

5. After finding the target, move the target so that it is in the middle of the field of view, and then use a high-magnification lens instead.

6 and repeat 4

Take out the microscope, first use a low magnification lens to align the light through the hole, adjust the light concentration of the mirror, then put the loading on the stage, let the objective lens fall, stick to the top of the loading, slowly rise while observing whether the image is clear, first use a coarse collimation spiral and then use fine calibration fine-tuning.

Material: Take the ideal biological tissue thin slice and dye it with dripping dye solution on the thin slice for 2-3min, and then wash off the floating color with alcohol.

2.It is made into a temporary film.

3.Observation: Observe under a low magnification lens first, adjust it with a coarse collimation spiral to find the material to be observed, and move it to the ** of the field of view.

4.Switch to high-magnification observation and adjust it with a fine collimation spiral to find the object to be observed.

1 Hold the arm with your right hand and hold the base with your left hand.

2 Place the microscope on the bench, slightly to the left (the microscope is about 7 cm away from the edge of the bench). Attach the eyepiece and objective.

2. Alignment 3 Rotate the converter to align the low-magnification objective with the clear aperture (the front end of the objective lens and the stage should be kept at 2 centimeters.)

meters).

4 Align a larger aperture at the aperture. The left eye is fixed inside the eyepiece (the right eye is open while the drawing is drawn). Turn the mirror so that the light is reflected into the barrel through the aperture. Through the eyepiece, you can see a white and bright field of vision.

3. Observation 5 Place the slide specimen to be observed (it can also be made of thin paper with the word "6" printed on it) on the stage, press it with a tablet clamp, and the specimen should be facing the center of the light hole.

6 Turn the coarse collimator spiral so that the lens barrel is slowly lowered until the objective lens is close to the slide specimen (eyes look at the objective lens so that the objective lens does not touch the slide specimen).

7 The left eye looks into the eyepiece and at the same time turns the coarse collimation spiral in the opposite direction so that the lens barrel slowly rises until the image is clearly visible. Then turn the fine collimation spiral slightly to make the image of the object seen more clearly.

Precautions: After the experiment, wipe the surface of the microscope clean. Turn the converter to offset both objectives and slowly lower the barrel to the lowest point. Finally, put the microscope in the case and return it to its original place.

1. To the light. 2. Place the film.

3. Use a low-magnification objective lens first to adjust the coarse quasi-focus.

4. Find the image of the object to be observed and move it to the field of view.

5. Use a high-magnification objective lens and adjust the fine focus.

6. Observe carefully.

1. Turn on the lighting system.

2. Put on the loading film.

3. The adjustment objective lens is a low magnification lens.

4. Adjust the coarse quasi-focal spiral to find the object.

5. Change to a fine quasi-focal spiral to find the object to be observed.

6. Replace it with a high-magnification lens, and adjust the fine-focus spiral at the same time to find a clear and high-magnification image.

Placing --- --- focusing --- observation of the light --- film.

That's it!!

Lens collection and placement and film mounting, observation and arrangement.

The loading should be moved to the upper right corner.

Analysis: The object image seen under the microscope is upside down, left and right, so when we move the slide specimen, the specimen moves in the opposite direction to the direction of the image. For example, if we move the slide specimen to the right, and the image we see in the microscope moves to the left, so when the image is on the upper right side of the field of view, if we want to move it to the field of view**, we should move the slide specimen to the upper right so that the image moves to the bottom left and moves to the field of view**.

The correct steps to use the microscope are: 1. Take the lens and place it.

1 Hold the arm with your right hand and hold the base with your left hand.

2 Place the microscope on the bench, slightly to the left (the microscope is about 7 cm away from the edge of the bench.

Install the eyepiece and objective

Second, to the light. 3 Turn the converter so that the low-magnification objective is aligned with the clear aperture (keep the front end of the objective at a distance of 2 cm from the stage).

4 Aim a large aperture at the aperture The left eye is fixed in the eyepiece (the right eye is open so that it can be drawn at the same time later) Turn the reflector so that the light is reflected into the lens barrel through the perforation Through the eyepiece, you can see the white vision

3. Observation Put the slide specimen to be observed (it can also be made of thin paper with the word "6") on the stage, press it with a tablet clamp, and the specimen should be facing the center of the light hole

6 Turn the coarse collimator spiral so that the lens barrel is slowly lowered until the objective lens is close to the slide specimen (eyes look at the objective lens so that the objective lens does not touch the slide specimen).

7 The left eye looks into the eyepiece, and at the same time rotates the coarse collimation spiral in the opposite direction to make the lens barrel rise slowly until the object is clearly seen

4. Finishing 8 After the experiment is completed, wipe the surface of the microscope clean Turn the converter, deviate the two objective lenses to the sides, and slowly lower the lens barrel to the lowest point, and place the mirror vertically Finally, put the microscope into the mirror box and send it back to its original place

So the answer is: (4)(3)(2)(1).

Take the lens, place it quietly, and observe the (light) and the film (focus).

Before observation, turn the quasi-focus spiral, when the lens barrel is lowered, the eye must look at the mirror from the side, so as not to let it change the group to contact the slide, otherwise it will crush (the glass slide) and damage the objective lens (lens).

Sections, smears.

Test Analysis: The microscope uses optical principles to imaging, which must allow visible light to pass through, and the observed material must be thin and transparent; If it is not transparent, light cannot pass through the material to be observed through the objective lens or eyepiece into the eye, forming an image image on the retina

There are three types of slide specimens: slices, smears, and mountings Slides are made from thin sections cut from living organisms Smears are made with liquid biological materials (e.g., bacterial cultures, blood) smeared Mountings are made with small amounts of material that are torn or picked from living organisms

Align the light first, put the loading into it, then adjust the coarse collimation spiral, and then adjust the fine alignment spiral until you can see it clearly.

When changing the high magnification lens, you can only adjust the alignment spiral.