How to increase the sensitivity of a compound to the protein of interest

To do gradient test, use the same secondary antibody concentration test, the concentration gradient of the analyte, and the evaluation index: blank ID, linear range, positive OD value, ratio (difference) between the OD value of blank ID, etc., depending on the application requirements.

There is no point in asking such a question.

It is advisable to go down and check the information yourself.

Copy of the internal reference protein.

The expression level is generally larger, and the antibody BAI of the internal reference protein is usually comparative.

du is easy to use. The expression of the target protein may be much smaller than that of the internal control protein, or it may be that the antibody to the target protein is not easy to use. Therefore, consider concentrating your protein sample (increasing the number of cells, reducing the lysate, etc.), increasing the concentration of the antibody to the protein of interest, and changing the antibody to the protein of interest.

The indole group of tryptophan has a maximum absorption peak at 280 nm, the phenol group of tyrosine has a maximum absorption peak at 275 nm, and the benzene ring of phenylalanine has a maximum absorption peak at 257 nm.

A: B is incorrect.

Protein molecules contain aromatic amino acids such as tyrosine and tryptophan with conjugated double bonds. They have the property of absorbing ultraviolet light, and their absorption peak is at the wavelength of 280 nm, and the optical density value of the absorption peak in this wavelength is proportional to its concentration.

The indole set does not contain a conjugated double bond, while the phenol group contains a conjugated double bond (contained in the benzene ring), so B is chosen

antigen-antibody hybridization with the corresponding antibody (against isotope labeling); If a hybridization band appears, it indicates that the gene of interest has formed a protein product.

This is called "prokaryotic expression". First of all, you need to have a prokaryotic expression vector, then you need to have the cDNA sequence corresponding to the protein, so as to construct a prokaryotic expression fusion vector, and finally transfer the constructed vector into an E. coli strain such as BL21, and induce expression by IPTG method.

Exercise is an activity that consumes energy in the body, in the process of your bodybuilding, one is to consume a lot of energy, in addition to carbohydrates and proteins play an important role in the formation of muscles, although supplementing carbohydrates and proteins are high-energy substances but consumed in the process of bodybuilding, this is the consumption and replenishment of energy, will not cause you to become fat, will only make your body bigger, and there is muscle, oh, this is how a good body comes.

Carbohydrates can be supplemented in a normal diet, such as staple foods that are rich in carbohydrates. Diet: Meat, fish, milk, beans, and eggs are rich in protein, and protein is needed to gain muscle. You can take the method of eating small meals often, and you should not eat too much at each meal.

There should be a gap of 30 minutes to 1 hour between exercise and eating, and the absorption is better than usual after 30 minutes of exercise. If it is inconvenient to eat, you can have a little protein powder or muscle building powder (thin people are more suitable to eat muscle gain powder first to gain weight).

It depends on your physique and fitness purpose.

The subject should understand this first: while drinking protein powder.

Version of eating carbohydrates, what is the purpose right? The goal is to make more efficient use of protein.

After a workout, the body's energy demand will not drop rapidly, but will remain at a high level for a period of time, burning carbohydrates and fats continuously. This process usually wanes down after the heart rate returns to a normal state.

Drinking protein powder and eating carbohydrates after training is to allow carbohydrates to provide energy for the process, rather than breaking down proteins to provide energy.

So, if you are obese and the purpose is to get in shape, it is best not to eat it.

If your goal is to gain muscle, then a moderate amount of supplementation is necessary, in moderation.

Definitely, just after practicing glucose deficiency, if you don't eat sugar, the protein will be converted into sugar, and you will drink it for nothing, or the efficiency of drinking is not high.

You can skip it, it depends on your taste.

Definitely to eat, protect the protein.