What is the difference between pathology section film and white film

No, the section here is the HE section of the original unit diagnosis, which is stained.

The white slice is cut from the same tissue block as the section, and the tissue is not stained, and it is estimated that the consultation unit will do immunohistochemical staining or FISH testing. So tell you to take the white sheets.

The so-called white slice refers to the ** that has been cut but has not been stained, which is a wax piece with a thickness of 3-5 microns cut from the wax block with a microtome, and then pasted on the glass slide.

The white film is unstained, and can be used for HE staining, immunohistochemical examination, special staining and other pathological and morphological examinations; There is no coverslip, so the tissue can be scraped off for molecular pathology such as genetic testing. Depending on the size of the tissue in the block, the larger the tissue, the greater the number of white slices that can be cut, and the less obvious the morphological change in each slice, and the smaller the tissue, the opposite. When you get the white film, you should pay attention to it, you can't touch the wax sheet with your hands, don't break the slide, and the two white pieces can't touch each other (except for back-to-back, the general pathology department will help you pack it).

What are white tablets and wax blocks.

Drawbacks of rapid pathology sectioning.

Are pathology sections 100% accurate.

Wax block pathology**.

Can pathology be done with only white films.

1. Examination is different: pathological section.

All of them can be stained with HE.

After that, if it is directly observed under the microscope, the patient will often borrow this kind of section and consult the branch resistance. The white slice is cut from the same tissue as the pathological section, and the tissue flake that has not been stained is not stained, and the thickness of the white slice of this slice will be different according to the different items detected by molecular pathology.

2. Different properties: take a certain size of diseased tissue and use pathological histology method to make pathological sections. Usually, it is necessary to go through the procedures of borrowing or cutting pathological white films in the hospital for pathological diagnosis, and the obtained pathological white films can be observed after HE staining or immunohistochemical staining in a higher-level hospital, so that pathological diagnosis can be carried out and an accurate pathological report can be obtained.

3. Different characteristics: some tissue blocks of pathological sections are extremely difficult to penetrate into the interior due to their large volume or fixative solution, or they need to fix the whole organ or the whole animal body. In this case, injection fixation or perfusion fixation should be used.

Pathological white film is usually used for consultation or immunohistochemical marking, which is recommended for consultation or cannot be done in this hospital.

All the same, the pathological sections were baked and stained, and the white sections were not.

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What is a pathology section and what is it for.

Dear, this is a professional question, I found you a doctor professional.

Points to note for pathological sections:The fresher the tissue, the better, the human tissue is generally in the body after the body, and the animal tissue is quickly fixed after the sacrifice to ensure the original morphological structure. The ideal volume of the tissue block is so that the fixative solution can penetrate into the tissue quickly and evenly.

However, according to the different materials and purposes of the production, the ideal volume of the tissue block is also different, such as the production of pathological external examination, scientific research section, the tissue block can be thin. Cut into thin slices on a microtome, adhered to the slide, dyed with various colors, for examination under the microscope to observe pathological changes, make pathological diagnosis, and provide help for clinical diagnosis and **.

Related information

Pathological section is a diagnostic method to surgically remove a part of the lesion site, and then observe the tissue lesion under the microscope, which is the exact basis for diagnosis, and there are still some differences between pathological section and biopsy. Biopsy is to directly examine the excised tissue under the microscope, while pathological section is to fix the excised tissue with some treatment, and then microscopic observation is more detailed.

It takes time to make pathological sections, and I hope to wait patiently, and the rapid sections in surgery are limited, but the results of the sections taken out are correct. Postoperative pathological sections will be collected from multiple points and more samples, and the examination of the entire specimen will be more thorough, so the accuracy will be higher.

The above content reference: Encyclopedia - Pathological Sections.

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