Solid phase extraction column specifications for solid phase extraction columns

Most of the common solid-phase extraction columns are syringe type devices made of polyethylene, which are equipped with two plugs made of polypropylene or glass fiber, and a certain amount of chromatographic sorbent (packing material) is contained between the two plugs. The upper end of the solid phase extraction column is open, and the lower end is the liquid outlet, and the liquid is discharged from the outlet after passing through the adsorbent. According to the volume of space above the packing material of the classical SPE column, the specifications of the SPI column can be divided into 1 ml, 3 ml, 6 ml, 10 ml, 15 ml, 20 ml, 30 ml, 60 ml, and so on.

There are also special specifications for SPE columns that can be manufactured according to specific application requirements. From the outside of the SPE column, the classic SPE column is similar to the injection syringe barrel and is a straight type. The other type of SPE column has a funnel-shaped upper end, which allows for the loading of more samples at a time during manual operation.

The non-fritted SPE column is a new type of integrated SPE column. The product uses Orbi Technology's exclusive process to specially treat C18 and C8 bonded silica gel fillers to make them have high mechanical strength and toughness, and there is no need to add polyethylene porous baffles to the column as support. The Orbi proprietary technology product "Integrated SPE Solid Phase Extraction Column" is the earliest integrated SPE column in the domestic and foreign markets.

Product advantages: 1. There is no sieve plate, which perfectly avoids the "non-specific adsorption" caused by the sieve plate. 2. The filler is stable and fixed, and there will be no common problems such as loose filler.

3. The integrated design of the overall filler effectively avoids the "ditch effect". 4. Packing with particle size as low as 8-10 can be selected, which greatly improves the separation efficiency and mass loading.

Don't buy Simon Aldrich's solid-phase extraction column, there is no such company at all, and the quality of small domestic workshops pretending to be imported brands is very poor.

The SPE column is neither an instrument nor a reagent, it is a common tool in chemical separation techniques. SPE columns typically consist of a stationary phase and a column packed in a column to enrich the compound of interest from the sample. The stationary phase of a solid-phase extraction column is typically a porous material such as silica gel, alumina, polyamide, etc., which has adsorption properties to adsorb the compound of interest.

When using a solid-phase extraction column, the target local modulo compound is adsorbed by the solid phase while other impurities are washed away as the sample passes through the column. Therefore, the solid-phase extraction column is a common tool in chemical separation techniques, and it is neither an instrument nor a reagent.

Summary. The SPE column can be seen as both an instrument and a chemical reagent. From an instrumental perspective, a solid-phase extraction column is a columnar device with a stationary phase, typically consisting of components required to connect to detection equipment such as a chromatograph or mass spectrometer.

Its main role is to extract and enrich compounds for subsequent steps such as separation, purification, and quantification. From a reagent perspective, the SPE column is equivalent to a small, stationary phase traditional separation column, which is filled with different types of adsorbent materials, and different types of stationary phases can be selected for different species characteristics for sample preparation and purification. Therefore, we can think of the SPE column as both an instrument and a chemical reagent.

The solid-phase extraction column can be seen as both an instrument and a chemical reagent. From an instrumentation perspective, a solid-phase extraction column is a columnar device with a stationary phase that typically consists of the components required to connect to detection equipment such as a chromatograph or mass spectrometer. Its main role is to extract and enrich compounds for subsequent steps such as separation, purification, and quantification.

From a reagent perspective, the SPE column is equivalent to a small, stationary phase traditional separation column, which is filled with different types of adsorbent materials, and different types of stationary phases can be selected for different species characteristics for sample preparation and purification. Therefore, we can think of the SPE column as both an instrument and early dross as a chemical reagent.

Fellow, I really didn't understand, I can be more specific.

The solid-phase extraction column can be seen as both an instrument and a chemical reagent. From an instrumentation perspective, a solid-phase extraction column is a columnar device with a stationary phase that typically consists of the components required to connect to detection equipment such as a chromatograph or mass spectrometer. Its main role is to extract and enrich compounds for subsequent steps such as separation, purification, and quantification.

From a reagent perspective, the SPE column is equivalent to a small, stationary phase traditional separation column, which is filled with different types of adsorbent materials, and different types of stationary phases can be selected for different species characteristics for sample preparation and purification. Therefore, we can think of the SPE column as both an instrument and early dross as a chemical reagent.

Summary. Solid-liquid extractionSolid-liquid extraction raw materials are mixtures of solutes and insoluble solids, where solutes are soluble components, and the face body is not referred to as a carrier or inert substance. A unit operation in which a solid mixture is impregnated with a solvent to separate soluble components and residues.

The materials to be processed by leaching include natural or pyrotechnic minerals, as well as biological substances, such as roots, stems, leaves, seeds, etc.

In most cases, one liquid phase is an aqueous solvent and the other is an organic solvent. The selectivity and separation efficiency of the extraction process can be controlled by selecting two incompatible liquids. In both the aqueous and organic phases, the more hydrophilic the hydrophilic compound, the greater the degree to which the hydrophobic compound will enter the organic phase.

Solid-liquid extractionSolid-liquid extraction raw materials are mixtures of solutes and insoluble solids, where solutes are soluble components, and the face body is not referred to as a carrier or inert substance. A unit operation in which a solid mixture is immersed in a solvent to separate soluble components and residues. The materials to be processed in leaching include natural minerals or minerals treated by fire starvation, as well as biological substances, such as roots, stems, leaves, seeds, etc. of plants.

In most cases, one liquid phase is an aqueous solvent and the other is an organic solvent. The selectivity and separation efficiency of the extraction process can be controlled by selecting two incompatible liquids. In both the aqueous and organic phases, the more hydrophilic the hydrophilic compound, the greater the degree to which the hydrophobic compound will enter the organic phase.

Depending on the resin retention mechanism, where the resin retains the compound of interest or impurities, the procedure varies slightly. There are generally four steps in the SPE operation:

1.The resin retains the compound of interest.

The activation --- removes impurities from the cartridge and creates a solvent environment.

--- loading, the sample is dissolved in a solvent, transferred to the column, and the components remain on the column.

Rinsing --- remove interferences to the greatest extent.

The analyte substance is eluted --- a small volume of solvent and collected.

2.The filler retains impurities.

There are generally three steps in the SPE operation:

Activation – Removes impurities from the column and creates a solvent environment.

Loading – The sample is transferred to the column, where most of the target compounds flow out with the sample base and impurities are retained on the column, so the collection begins at this step.

The components are washed down with a small volume of solvent --- eluted and collected, and the collected solution is combined.

This condition is mostly used to remove pigments in food or pesticide residue analysis.

Compared with the traditional liquid-liquid extraction method and protein precipitation method, solid-phase extraction has incomparable advantages, such as early cavity

Purification Method: LLE (Liquid-Liquid Extraction).

Pros: No special devices required.

Cons: 1The operation is cumbersome and time-consuming.

2.It requires a large amount of organic solvents, resulting in high costs and environmental pollution.

3.It is difficult to extract highly water-soluble substances from water.

Purification method: PPT (protein precipitation).

Advantages: Easy to operate, no special devices required.

Cons: 1Non-specific precipitation reactions may result in the loss of trace amounts of analytes as matrix proteins co-precipitate.

2.The purification effect is weak, and the detection sensitivity and reliability are low.

SPE (Solid Phase Extraction).

Advantages: 1. It can complete sample enrichment and purification at the same time, which greatly improves the detection sensitivity.

2. It is faster than liquid extraction, more solvent-saving, and can be processed in batches.

3. Good reproducibility.

Cons: 1The cost of using imported solid-phase extraction cartridges is high.

2.Professional assistance is required for method development.

The selection of a good extraction column is generally considered from two aspects, namely the sorbent and the column size.

Suitable adsorption fillers can achieve good separation of interfering substances from target components and meet the best requirements. However, the amount of compound adsorbed by the sorbent is limited, and if the sample volume is too large, there will be "overload", so that the target will "penetrate" directly through the column and not be retained.

The adsorbent can be selected mainly according to the properties of the target compound and the matrix, and for specific examples, the selection can be based on the principle of "distinguishing the target compound from the main interference".

Once the appropriate sorbent has been selected, it is also necessary to select the appropriate column format based on the amount of sample required for the characterization. Since the mass of compounds that can be adsorbed by the sorbent of the extraction column is limited, once this value is exceeded, the target cannot be effectively retained, which greatly reduces the elution effect. Therefore, it is necessary to select the appropriate column format based on the amount of components in the sample.

There are many types of solid-phase extraction columns, and in specific experimental work, it is necessary to reasonably select the appropriate packing and reasonable specifications of the solid-phase extraction column according to the analysis object, detection method and laboratory conditions.