Bacteriophage infects bacteria。。。。。。。 Ask for help

None of the protein molecules of the progeny phages have the element S-35, thus indicating that the vast majority of the protein moiety of the phage does not enter the bacterial cell

Note: Some extremely individual enzymes, such as reverse transcriptase of RNA phages, will still drop in.

The protein molecules of the progeny bacteriophages all contain the element and this is because"

Second, did you make a mistake? That's generally what I ask:

The nucleic acid molecules of the progeny phages all contain the element p-32 because the nucleic acids of the phages enter the bacteria

Proteins do have phosphorylation. And it's widespread. Phosphorylation and dephosphorylation are widely present in cell signaling and in the amplification of the information transfer cascade.

But the phosphoric acid content of proteins is very low, and even after phosphorylation, there is at most one or two phosphorus in tens of thousands of atoms. The sensitivity of radiography is far from sufficient relative to this femtogram amount. Western blotting with autoradiography imaging is applied, which is two cascades of magnification with primary and secondary antibodies, magnified millions of times.

Even so, Western is now starting to use fluorescent dyes, gradually phasing out radiographiography because fluorescent dyes can use the action of luciferase-catalyzed substrates for an additional cascade amplification.

As mentioned in the textbook, the method of direct film shooting by radioautoradiography is still a technology decades ago, and the sensitivity is very low, and radioactive isotopes below 50ng cannot be displayed at all, so it is impossible to shoot phosphorus isotopes in ordinary proteins that are more than 1,000 times lower than this.

Hershey (1908c) and Chase (cultured the host bacteria in a medium containing 35s (indicating isotopes) and 32p, respectively, and the host bacteria were labeled with 35s and 32p, respectively, during the growth process. Hershey et al. then used T2 phage to infect the bacteria labeled with 35S and 32P, respectively. Bacteriophages proliferate within bacterial cells and release many progeny phages after lysis, in which the former is labeled by 35s and the latter by 32p.

The second step of the isotope labeling experiment is to infect the unlabeled bacteria with phages labeled by 35S and 32P, respectively, and then measure the isotope labeling of the host cell When infecting bacteria with a 35S labeled phage, the assay results show that there are few isotope labels in the host cell, and most of the 35S-labeled phage proteins are attached to the outside of the host cellWhen the bacteria are infected with the 32P-labeled phage, The assay results showed that very little radioisotope 32p was present in the outer phage shell of the host cell, while most of the radioisotope 32p was inside the host cell. The above experiments show that when phages infect bacteria, it is mainly DNA that enters the bacteria, while most proteins are outside the bacteria.

You're right, I'm sure.

Adsorption, injection, synthesis, assembly, release. Bacteriophages are viruses that invade bacteria and are also the genetic material that gives biological traits to the host bacteria. Bacteriophages must parasitize within living bacteria and have strict host specificity, which depends on the molecular structure and complementarity of phage adsorption organs and receptors on the surface of the recipient bacteria.

Bacteriophage is a general term for viruses that infect microorganisms such as Sclerotiobacterium, fungi, algae, actinomycetes or spirochetes, and are called bacteriophages because some of them can cause lysis of host bacteria. It was first discovered in staphylococci and shigella at the beginning of this century.

As a type of virus, bacteriophages have some of the characteristics of viruses: tiny individuals; does not have an intact cellular structure; Contains only a single nucleic acid. It can be thought of as a creature that "preys" on bacteria.

The phage genome contains many genes, but all known phages are bacterial cells that use the ribosomes of bacteria, various factors required for protein synthesis, various amino acids, and energy production systems to achieve their own growth and proliferation. Once out of the host cell, the phage can neither grow nor replicate.

Bacteriophage is a kind of virus, and its special feature is that it specializes in bacteria as the host, and the more well-known bacteriophage is the T2 bacteriophage with Escherichia coli as the host. Like other viruses, a bacteriophage is just a mass of genetic material wrapped in a protein shell, and most phages have a "tail" that is used to inject genetic material into the host.

A bacteriophage is a ubiquitous organism and is often accompanied by bacteria. Bacteriophages can usually be found in places full of bacterial communities, such as soil and the internal organs of animals. The richest place in the world to have bacteriophages is seawater.

1.The phage infection experiment was done by Hershey and Chase.

2.Hershey and Chase were both members of the phage team.

3.In 1952, they conducted the famous bacteriophage infection experiment, which proved that DNA is genetic material.

4.A bacteriophage is a virus that parasitizes the cells of bacteria and destroys them.

5.Bacteriophages are very small in size and very simple in composition, both composed of proteins and DNA.

6.The structure of the bacteriophage is that the outer bread has a shell of the white matter of the egg, and the substance tightly wrapped inside is DNA.

7.It acts like a syringe, attaching itself to the outer surface of the hunger-reeling bacteria and then injecting its internal material (DNA) into the bacteria.

Protein composition: C, H, O, N, S, so 35S is labeled on the phage shell and DNA has a phosphate group, so 32P is labeled on the phage DNA.

Bacteriophage infection of E. coli is done by injection, so the outer shell remains outside E. coli and only DNA is injected into E. coli.

The E. coli itself is not radioactive, and the phage uses the substance of E. coli to synthesize its own components, so after the lysis of E. coli, the phage produced is not 35s, only the phage itself (parent) contains 32p, so radioactive phosphorus can be detected.

In fact, the most important thing in the experiment of phage infection bacteria is that the head (protein) of the phage is left outside, the DNA is released into the bacteria for replication, and then the protein synthesis is directed, and then the newly synthesized protein and DNA are reconstituted into new phages and released from the bacteria.

What is the process of phage infiltrating bacteria.

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