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Preparation of phosphate buffer (PB).
Reagent: NaH2PO4·2H2O NA2HPO4·12H2O
Preparation method: When preparing, NaH2PO4 and Na2HPO4 are often prepared first, and the two are mixed in a certain proportion to form phosphate buffer (PB), and different concentrations of PB and PBS can be prepared according to the need.
1) of NAH2PO4; Weigh or repopulate NaH2PO4·H2O to 1000ml to dissolve.
2) Na2HPO4: Weigh Na2HPO4·12H2O or Na2HPO4·7H2O or Na2HPO4·2H2O Aggravated distillation to 1000ml to dissolve.
3) Preparation of PB: Take 19ml of NaH2PO4 and 81ml of Na2HPO4·12H2O, and mix well to form PB (pH approx. If the pH is high or low, it can be adjusted by changing the ratio of the two and stored at room temperature.
Phosphate-buffered saline (
ph nah2po4(ml) na2hpo4(ml)
Also: preparation of phosphate buffered saline (PBS).
Reagent: PB 50ml
NACL approx.
Re-steam water to 1000ml
Preparation method: Weigh 50ml of NaCl and PB, add it to a 1000ml volumetric flask, and finally increase the steaming water to 1000ml, and shake it well. If the PBS to be prepared, the amount of PB can be doubled, and so on.
PB and PBS are the most commonly used buffers in immunocytochemistry experiments, PBS is mainly used for rinsing tissue specimens, diluting serum, etc., and its pH should be between, otherwise it needs to be adjusted. PB is often used to prepare fixatives, sucrose, etc. In general, the pH value of PB is slightly higher, and when diluted into PBS, the required pH value can often be reached, and if the pH needs to be adjusted, the pH of PB is usually adjusted.
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It's called sodium dihydrogen phosphate dodecahydrate, do you want to ask other common names? Yes and no.
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Sodium carbonate is a white powdery substance, soluble in water, alkaline.
When sodium carbonate crystallizes and precipitates from the solution, a certain number of water molecules are bound to the crystal, and such water molecules are called crystal water.
The chemical formula of sodium carbonate crystals is Na2CO3·10H2O, which means that one sodium carbonate molecule can bind 10 water molecules.
Substances that contain crystal water are called crystal hydrates.
Sodium carbonate crystals Na2CO3·10H2O are pure, not mixtures. How many water molecules are bound is determined due to the structure of the sodium carbonate itself. 10 means that one sodium carbonate molecule in the sodium carbonate crystal binds 10 water molecules.
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This is sodium carbonate decahydrate, which is pure, not a mixture. This substance refers to sodium carbonate with ten crystalline waters, which is pure. It is hydrate, and hydrate is not a mixture, it is pure. Sodium carbonate and water are bonded together in a strong chemical bond.
Name: Sodium carbonate decahydrate.
Molecular formula: Na2CO3·10H2O
Properties: also known as edible flour alkali, the main ingredient of flour alkali. It belongs to salt, not alkali.
White monoclinic crystals. Density. Melting point.
Heat until dehydration begins. Soluble in water, high solubility in hot water. Insoluble in alcohol.
It quickly weathers in the air to form a white powder. The aqueous solution is weakly alkaline. The reaction with acid neutralization reacts to release carbon dioxide.
Soda ash and sodium sulfate are added with water, and the solution is cooled and crystallized at a lower temperature in proportion. It is mainly used for pasta fermentation, and is also used for home washing and wool washing.
Also known as: sodium carbonate crystal, which is the crystalline form of Na2CO3.
Sodium carbonate decahydrate is heated to prepare sodium carbonate:
Heating) Na2CO3 + 10H2O
weathering). In fact, it is the cause of weathering.
Sodium carbonate decahydrate does not react with steel or iron.
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Summary. Hello dear, happy to answer your <>
According to chemical nomenclature, the molecular formula of a compound uniquely determines its chemical composition and structure. For the molecular formula of C6H12O2N, we can analyze:1
It contains four elements: carbon C, hydrogen H, oxygen O, and nitrogen N. 2.The quantity of carbon is 6, the quantity of hydrogen is 12, the quantity of oxygen is 2, and the quantity of nitrogen is about 116 based on the molecular formula.
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<> What is the molecular formula of C6H12O2N?
Hello dear, happy to answer your <>
According to chemical nomenclature, the molecular formula of a compound uniquely determines its chemical composition and knot-and-absence bond structure. For the molecular formula of C6H12O2N, we can analyze:1
It contains four elements: carbon C, hydrogen H, oxygen O, and nitrogen N. 2.The quantity of carbon is 6, the quantity of hydrogen is 12, the quantity of oxygen is 2, and the quantity of nitrogen is about 116 based on the molecular formula.
Oh [fresh stool pretending to disturb flowers] <>
Kiss, and then there is <>
<> after comprehensive analysis, it is very likely that C6H12O2N refers to the amino acid molecule alanine. Alanine is a non-essential amino acid, and its molecular formula is C6H12O2N, which is relative to the Huai Selling Molecule and has a mass of 116. Conclusion:
C6H12O2N is the chemical formula of propyl lead <>
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Na2S2O4, chemically known as sodium dithionite, is an inorganic compound. It is a colorless crystalline solid, often in the form of Siheng white powder. Sodium sulfite is soluble in water and has good reducing and decolorizing properties.
Sodium sulfite has a wide range of applications in chemical and industrial fields. One of the most common applications is as a reducing agent. It can reduce certain substances to a lower oxidation state, so it is commonly used in the dye industry, paper industry, and bleaching processes.
Sodium sulfite is effective in removing stains and pigments, fading dyes and pigments, and can also be used to bleach pulp and cellulose materials.
In addition, sodium sulfite is also used in the field of environmental protection. It can be used to treat heavy metal ions in wastewater and exhaust gases, reducing them to a relatively harmless form. In addition, it can be used as an intermediate in catalysts, antioxidants, and vulcanizing agents.
Although sodium sulfite has important applications in many fields, it also needs to be used and handled correctly. Sodium sulfite is a strong reducing agent, and it is easy to react with oxygen to form sulfite. Therefore, appropriate safety measures should be taken during storage and use to avoid contact with the air.
All in all, sodium sulfite is a common chemical that is widely used in chemical, industrial, and environmental protection fields. Its reductive and deoxidizing properties make it an important industrial chemical. However, safety should be taken into account when using and proper handling and disposal methods should be followed. <>
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Na2C2O4 is sodium oxalate
H2C2O4 is oxalic acid, also known as adipic acid, an organic acid. Na2C2O4 is sodium oxalate, a salt. Spinach and tofu cannot be eaten at the same time, because spinach contains more oxalic acid, which will react with the calcium in tofu to produce insoluble calcium oxalate.
Common chemical names:
Baking soda:
Sodium bicarbonate, with the molecular formula NaHCO, is an inorganic salt, white crystalline powder, odorless, alkaline, soluble in water. It decomposes slowly in moist or hot air to produce carbon dioxide, which is heated to 270 degrees Celsius and completely decomposed. In case of acid, it decomposes strongly to produce carbon dioxide.
Sodium ions are a normal requirement of the human body and are generally considered non-toxic.
However, excessive intake may cause alkalosis, damage the liver, and induce high blood pressure. Sodium bicarbonate solids begin to gradually decompose above 50 to form sodium carbonate, water and carbon dioxide gas, and this property is often used as a leavening agent for making biscuits, pastries, steamed bread and bread. Sodium bicarbonate will remain sodium carbonate after action, and too much use will make the finished product have an alkaline taste.
Baking Soda:
Sodium thiosulfate, also known as sodium hyposulfite, baking soda, hypo, molecular formula: Naso. It is a common thiosulfate, colorless crystalline or white particle.
Sodium thiosulfate is easily soluble in water and reacts with strong acids to produce sulfur and sulfur dioxide. Sodium thiosulfate is colorless, transparent crystalline or crystalline fine particles, odorless, salty, weathering in dry air, deliquescent in humid air, and weak alkaline reaction in aqueous solution.
Aqua regia:
Also known as royal acid and nitro hydrochloric acid, it is a very corrosive, yellow liquid with a yellow mist, which is a mixture of concentrated hydrochloric acid (HCL) and concentrated nitric acid (HNO) with a volume ratio of 3:1.
Aqua regia is generally used in the etching process and some detection and analysis processes, but some gold quarrels are elemental such as tantalum, iridium, candles and other advanced alkanes, and the king of plastics in organic matter - PTFE, silicon and other substances are not corroded by aqua regia, and inorganic salts such as aplatin sulfide and barium sulfate are also insoluble in aqua regia. Aqua regia is highly perishable and smells of chlorine, so it must be made and used freshly.
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NH4)2S2O4 is even ammonium disulfate, also called ammonium thiosulfate; (NH4)2S2O8 is ammonium perdisulfate, they are different substances, and there is nothing to do with it. When heated to 150, it decomposes to form ammonium sulfite and sulfur.
Ammonia, hydrogen sulfide.
and water. It is very unstable in the air and stable in the ammonia gas of Heba.
Chemistry is a natural science based on experiments. Mendeleev proposed a periodic table of chemical elements.
It greatly contributed to the development of chemistry. Nowadays, many people call chemistry the "science of the center of skating" because chemistry is at the heart of some scientific disciplines, such as materials science, nanotechnology, biochemistry, etc.
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