What are the uses and precautions of high magnification microscopes?

After years of research and development, Quantum Design has launched the FusionScope multi-function microscope, which is a pioneering combination of scanning electron microscope (SEM) and atomic force microscope (AFM) technology in one device. Without moving samples or switching operating systems, the complementary comprehensive measurement of SEM and AFM can be quickly realized in the same user interface and the same spatial location, and the physical properties of the selected area such as 3D topography, composition, mechanics, chemistry and electricity can be tested in situ, which pushes the surface characterization of samples by AFM and SEM to a wider infinite possibility! In most cases, a variety of techniques are often used to confirm the correlation between different parameters.

For AFM and SEM imaging, this means that the exact same area needs to be performed in practice....

Usage: Observe with a low magnification lens first Find the object to be observed, move it to the field of view** Turn the converter, observe with a high magnification lens, and gently adjust the fine collimation spiral to make the object clear. Notes:

When adjusting the coarse collimation spiral descent, pay attention to the distance between the objective lens and the slide; When using a high magnification lens, the coarse collimation spiral cannot be rotated.

1.Light microscopy.

The key points and precautions of the medium and high magnification objective lens are as follows.

First, when using a high magnification lens, it is necessary to first use the low magnification lens to find the target to be observed, move it to the center of the field of view, and then rotate the side lifter changer.

Observe with a high magnification lens. Swift enlightenment.

Second, after changing the high magnification lens, only the fine collimation spiral can be rotated, and the coarse collimation spiral cannot be used.

Third, after changing the high magnification lens, the field of view becomes darker, and you can use a wide aperture.

Fourth, the eyepiece magnification must be less than the objective lens magnification.

How to use a high-powered microscope:

Pick up and place the lens: Hold the arm with your right hand and drag the lens holder with your left hand. Place the microscope about 7 cm from the edge of the bench, slightly to the left. Anchang should pack the eyepieces and objective lenses.

Alignment: Turn the converter so that the low-magnification objective is aligned with the clear aperture (keep the front of the objective at a distance of 5 to 10 mm from the stage). Aim a larger aperture at the clear aperture.

One eye is fixed on the eyepiece and the other eye is open. Turn the mirror so that the light is reflected into the barrel through the aperture. Through the eyepiece, you can see the white, round optic plexus limb field.

Observation: Place the slide specimen to be observed on the stage and press it with a tablet clamp facing the center of the aperture. Turn the coarse collimation spiral so that the lens barrel is slowly lowered until the objective lens is close to the slide specimen.

One eye looks inside the eyepiece, and at the same time rotates the coarse quasifocal spiral in a counterclockwise direction so that the lens barrel slowly rises until the object is clearly visible. Then turn the fine collimation spiral slightly to make the object more clear.

High magnification microscope note: first observe with a low magnification lens, find the object to be magnified and observe, move to the field of view**, and then replace it with a high magnification objective. After changing to a high-powered objective, the coarse collimation helix can no longer be rotated, but can only be adjusted with a fine collimation helix.

How to use the microscope:

Steps to use the high magnification lens (pay special attention to steps 1 and 4).

1) Find the object under low magnification adjustment (coarse collimation spiral and fine collimation spiral) and move the object image to (field of view**).

2) Rotate the ** changer) and replace it with a high magnification lens.

3) Adjust (aperture) and (reflector) to make the field of view bright.

4) Adjust (fine focus spiral) to make the image clear.

Summary of how to use the microscope:

1. One take two put, three install, four turn low times, five pairs of light, six upper slides, seven down, eight liters of the tube, watch carefully, after reading the low times, turn high times, nine back!

2. When using high magnification, you should first use low magnification to find the target to be observed, and the observed specimen should choose an area without overlapping cells.

3. If the lens is dirty, it cannot be wiped with hands or with a cloth, and a special lens wiping paper should be used to wipe it.

4. The uneven thickness of the specimen cut leads to a clear part of the image and a blurry part. Poorly adjusted mirrors can result in a vision that is half bright and half dark.

Knowledge points: 1) When the microscope is aligned with light, the reflector or aperture should be rotated to make the field of view bright and easy to observe with a high magnification lens.

2) When making temporary mounting, if the plant cells are observed, add water dropwise to the slide; If human oral epithelial cells are observed, a solution of NaCl with a mass fraction of NaCl is required.

3) Whether animal tissue cells or plant tissue cells are selected, the amount must be small, and the material should be observed on the slide for easy observation.

4) When observing, follow the order of first observing clearly under the low magnification, moving the image to the field of view**, and then switching to the high magnification lens for observation. When observing with a high magnification lens, the coarse collimation spiral cannot be turned.

1.Plug it in.

2.Use a low-magnification observation to find the target you want to observe and move it to the center of the field of view (you can skip it if you become proficient).

3.Gently turn the nosepiece converter, move the high magnification lens to the working position, dim the light intensity appropriately, and then fine-tune the fine adjustment knob to make the object clear.