Edible mushroom tea tree mushroom cultivation, which position is best to isolate the mushroom body

1. If you want to cultivate tea tree mushrooms, then it is recommended to buy cultivated seeds directly, or buy mother seeds and original seeds and then multiply. Because, first, for novices, it is necessary to directly purchase cultivated seeds for cultivation, avoiding the early seed production process, the success rate of cultivation is high, and the strains are not expensive; Second, the multiplication of strains is easier than that of strain isolation, and the disinfection and sterilization of explants are involved in strain isolation, and the chance of contamination failure is high. Third, under normal circumstances, the variety and vitality of the purchased strains can be guaranteed (except for profiteers), and the pure mycelium made by themselves is relatively difficult to control in terms of variety and vitality.

2. If the pure mycelium is indeed made by itself, and the tissue separation method is adopted, then in terms of the explant site, the fungus meat at the junction of the stipe and the fold is the best (theoretically, any part of the fruiting body is fine). Because first, the new hyphae are robust and vigorous, which is suitable for separating materials; Second, the place is not easy to be contaminated by miscellaneous bacteria, and the success rate of inoculation is high.

3. As far as the preparation of pure mycelium by tissue separation method is concerned, in addition to the selection of explants, the more important thing is the pollution control of the whole link, from explant disinfection to aseptic operation, most of the failures of strain isolation are due to the failure to control the pollution.

Choose the fruiting body that you think is good, not too old, and isolate the joint site of the stipe and cap into the test tube according to the convention, which is the original mother species. Later, the well-growing peripheral hyphae were selected as the first generation of mother species.

Isolation of mushroom tissues from tea tree mushrooms. Fruiting bodies with excellent traits should be selected. Then select the junction site of the stipe and the cap. Under sterile conditions, intermediate meat can be picked and inoculated on the medium.

Tip of the stipe. Pull the stipe from the cap, then use scissors to cut off the flesh under the top of the stipe and transfer it to the PDA.

The stipe cap junction will be fine. It's as simple as that, what to do with so much nonsense, and it's not to write **.

Edible mushrooms were cultivated to produce mushroom denominators, original seeds and cultivated species;

Parent species: Species isolated directly from mushrooms (in vitro, cultured in culture medium).

Original species: the strain obtained by the expansion and propagation of the mother species (bottled species, cultured with culture materials, generally cultured in canned bottles or small bags).

Cultivated species: the strains obtained by the expansion and propagation of the original seeds (bagged seeds, cultured with culture materials, generally cultivated in mushroom bags) The mother seed is used to preserve the strain, the original seed is used to expand the strain, and the cultivated seed is used for production.

There are 5 commonly used methods for edible mushroom cultivation:

1 bag cultivation method. 2. Bed cultivation method.

3. Furrow planting method. 4 box cultivation method.

5. Potted cultivation method. Specific cultivation techniques can be found on the Internet.

When cultivating tea tree mushrooms, it is necessary to select strains with excellent varieties and high yields, and then use sawdust, cottonseeds, and wheat bran to mix and prepare into a cultivation substrate, and then inoculate the strains of tea tree mushrooms on the cultivation substrate, and control the ambient temperature between 23 degrees, and wait for about 60 days, and the tea tree mushroom will emerge.

1. Strain selection

When cultivating tea tree mushrooms, it is necessary to choose excellent and high yield varieties, the main varieties of tea tree mushrooms in China are Sanming tea tree mushrooms and Guangchang tea tree mushrooms, among which Sanming tea tree mushrooms have high yield, strong high temperature resistance, but poor taste, while Guangchang tea tree mushrooms have a good taste and delicate flesh, but the yield is low.

2. Cultivation medium

Tea tree mushroom is suitable for growing in loose and breathable soil, when cultivating tea tree mushroom, you can use sawdust, cottonseed, wheat bran, and seal to mix to make a cultivation medium, and then fill the cultivation medium with calcium carbonate to make it more fertile, and then the cultivation medium is sterilized at high temperature to make the tea tree mushroom grow healthily.

3. Cultivation method

When cultivating tea tree mushrooms, you need to put the cultivation medium into a sterilization bag, and then inoculate, and then put the tea tree mushroom into the inoculation box for disinfection, so that the probability of infection of the plant can be reduced.

4. Maintenance management

When maintaining tea tree mushrooms, it is necessary to adjust the indoor temperature to about 23 degrees to maintain the normal growth of the plant, and the indoor humidity can not exceed 65%, so as not to rot the tea tree mushrooms, after 30 days of breeding, the bag mouth of the sterilization bag needs to be loosened, so that a small amount of air enters the bag to promote the vigorous growth of tea tree mushrooms.

The management of tea tree mushrooms is generally divided into several steps: during the cultivation period, special attention should be paid to the temperature of the mushrooms, and the season of raising the mushrooms should be selected. Mushroom management, mushroom hygiene, pay attention to three elements, namely:

The temperature, humidity, and ventilation of mushrooms, the so-called ventilation is ventilation, which can replace the air in the greenhouse and increase the oxygen content in the greenhouse. To prevent insect pests, we can choose to use the power grid for prevention and control, or we can choose to use mosquito incense to control insect pests. Finally, it is dried and stored.

The general storage time of dry products is half a year, and the storage time of fresh products is about one week.

Fertilization requires a combination of basal fertilizer and top dressing, organic fertilizer and inorganic fertilizer, root and foliage. Sprinkler irrigation in tea plantations in high temperature and dry season is conducive to improving the microclimate and has a significant effect on improving the yield and quality of tea. Grass or mulching in tea plantations is an effective measure to prevent soil erosion and improve soil fertility.

Canopy management includes pruning, picking, etc. Generally in the early years of 2 to 4 times of stereotyped pruning. The height of the first pruning is about 15 cm above the ground, and the subsequent pruning is increased by 15 cm each time to cultivate dry branches and control the height of the crown.

The height of a single tree planted is controlled at 70-90 cm, and the height of dwarf dense trees is controlled at 55-65 cm.

In adulthood, light pruning is carried out on the canopy surface every 1 2 years with a depth of about 2 3 cm, and the top layer of green stem leaflets is cut off, so that the new shoots can grow vigorously and are easy to pick. Heavy pruning is carried out every 20 years in old age, and the canopy is rejuvenated by cutting it flat at a distance of 30 to 40 cm above the ground. The senescent tea trees are mowing about 10 cm above the ground to completely renew the canopy.

The height of the arbor type tea tree platform is about 20 cm; Picking is also an important means of cultivating the canopy, which should be closely coordinated with pruning. After stereotypical pruning, heavy pruning or table cutting, the tea tree should pick less leaves and leave more leaves, so that the crown can grow rapidly; If you cut lightly, you can pick more and keep less, leaving only fish leaves or one leaf, so that the output continues to grow. Pest and disease control.

The more common pests and diseases are anthracnose, tea caterpillar, tea inchworm, tea weevil, false eye small green leafhopper, tea orange gall mite, etc.

Tea tree mushroom has high nutritional value, how to cultivate tea tree mushroom? The cultivation of tea tree mushrooms has to be done in the following aspects, the first is to select the seeds, choose the most advanced species, and the age of the fungus is not more than 80 days. It is suggested that mushroom farmers directly introduce mother species from formal units to multiply.

The second is inoculation, the residual temperature of the mushroom tube during inoculation can not exceed 36, after the temperature is lower than 30, the mushroom tube is put into the inoculation box, and then after disinfection, the mouth of the bag should be close to the flame of the alcohol lamp, the mouth of the bag is facing down, except for a small amount of bacteria into the hole, most of the strains are placed on the surface of the medium, and then the bag mouth is tightened, and a bottle of production can be connected to the bag of 20-25 bags. The third is culture, after inoculation, put the fungus bag into the mushroom rack of the culture room for culture, and the room should be kept about 23. The relative air humidity should be kept no more than about 65%.

The indoor light should not be too strong, ventilate in the morning and evening, clean frequently, and the mycelium will grow for about 30 days, which can untie the tie line and enter a small amount of air to make it grow faster. The general temperature is suitable, about 60 days, and the mushrooms can be opened and produced.

First of all, maintain the proper temperature, tea tree mushrooms generally prefer to grow in temperate and tropical regions, resistant to low temperatures, high temperatures can also, in general, tea tree mushrooms can grow at a temperature of 5 to 35 degrees, usually the best growing temperature is 18 - 28 degrees, it belongs to the constant temperature of the development of mushrooms, does not need a large temperature difference, can grow faster.

Secondly, pay attention to maintaining sufficient water, the cultivation of tea tree mushroom needs to control 65% of the water, and then its growth will be relatively fast, generally speaking, its medium needs to maintain proper humidity, which is conducive to its growth, conducive to the growth and development of fungi.

Again, pay attention to keep enough air, the growth of tea tree mushrooms, need enough oxygen, usually to keep ventilation, and then in the mushroom, it still needs some more carbon dioxide concentration, can help it grow the stalk, and then increase the yield, so reduce the degree of ventilation in normal times, especially suitable for the growth of tea tree mushrooms.

First of all, choose a good variety of strains, and then choose a good fertile land and plant it well.

Grow it in a greenhouse, so that the temperature and humidity can be controlled, so that it can grow well.

It needs to be planted in winter and spring to have a higher yield, because the weather in these two seasons will be warmer, there will be no banks and no scorching heat, which will be more conducive to the precipitation of nutrients of tea tree mushrooms.

It is a kind of edible fungus, for the temperature to be controlled, in the time period of spore germination, it should be controlled at 26 degrees, and when it is obvious to see the appearance of mycelium, it is necessary to control the temperature separately, it should be kept at about 25 degrees, remember that it can not exceed more than 30 degrees, otherwise it will stop growing. Finally, it is necessary to supplement sufficient nutrition, its ability is somewhat weak, but protease has strong activity, so during the breeding period, some nutrients should be added and cultivated according to the normal ratio, so that the yield can be effectively improved.