How to focus when using a microscope, how to adjust the light and adjust the focus using a microscop

Hold the temple arm with your right hand and hold the lens base with your left hand.

2 Place the microscope on the bench, slightly to the left (the microscope is about 7 cm away from the edge of the bench). Attach the eyepiece and objective.

2. Alignment 3 Rotate the converter to align the low-magnification objective with the clear aperture (the front end of the objective lens and the stage should be kept at 2 centimeters.)

meters).

4 Align a larger aperture at the aperture. The left eye is fixed inside the eyepiece (the right eye is open while the drawing is drawn). Turn the mirror so that the light is reflected into the barrel through the aperture. Through the eyepiece, you can see a white and bright field of vision.

3. Observation 5 Place the slide specimen to be observed on the stage and press it with a tablet clamp, and the specimen should be facing the center of the light hole.

6 Turn the coarse collimator spiral so that the lens barrel is slowly lowered until the objective lens is close to the slide specimen (eyes look at the objective lens so that the objective lens does not touch the slide specimen).

7 The left eye looks into the eyepiece and at the same time turns the coarse collimation spiral in the opposite direction so that the lens barrel slowly rises until the image is clearly visible. Then turn the fine collimation spiral slightly to make the image of the object seen more clearly.

Precautions: After the experiment, wipe the surface of the microscope clean. Turn the converter to offset both objectives and slowly lower the barrel to the lowest point. Finally, put the microscope in the case and return it to its original place.

If you are only focusing, use a coarse collimation spiral to lower the lens barrel until it is close to the slide. When adjusting, the eyes should look at the lens tube. Then look at the eyepiece and adjust the barrel up with a coarse collimation spiral until you can see it almost clearly, and adjust the field of view with a fine collimation spiral until it is clear.

Any other questions you can ask me, I hope it will be helpful to you.

The scope of your question is large and the information is not very clear. Are you using a monocular or binocular microscope, natural light or electric light sources? The purpose of focusing is to make the image clearer, but to make the image clear is not just about focusing, you also need to adjust some other components.

Condensers, Kohler lighting, and more. Pay attention to all these aspects!

1.Alignment: Rotate the coarse collimation spiral to make the lens barrel rise slowly, and then rotate the converter to align the low-magnification objective lens with the clear hole; Turn the light shield (or sheet aperture) with your fingers so that the maximum aperture is aligned with the aperture, and the left eye is fixed into the eyepiece, while turning the reflector so that it is facing the light source so that the brightness in the field of vision is uniform and appropriate.

2.Use of the low-magnification objective: Turn the coarse collimation spiral by hand so that the barrel is slowly lowered while looking at the objective lens from the side with both eyes, stopping when the objective lens is 2 3 mm away from the slide on the stage.

Gaze into the eyepiece with your left eye (note that your right eye should be open at the same time) and turn the coarse focus spiral so that the lens tube rises slowly until you see the object clearly. If it is not clear, the fine collimation spiral can be adjusted until it is clear.

3.Use of high-magnification objective: Before using the high-magnification objective, you must first use the low-magnification objective to find the observed object, adjust it to the positive ** of the field of view, and then turn the converter and change to the high-magnification lens.

After switching to a high magnification lens, the brightness in the field of view becomes darker, so it is common to use a larger aperture and use the concave surface of the mirror, and then adjust the fine collimation spiral. ** The number of objects is smaller, but the volume is larger.

4.Use of reflectors: Mirrors are often used in conjunction with a light shield (or aperture) to adjust the brightness within the field of view.

Mirrors are available in both flat and concave surfaces. When lighting up, use the plane of the mirror if the light is too strong in the field of view, and use a smaller aperture at the same time if the light is still too strong; Conversely, if the light in the field of view is weak, use a wider aperture or use the concave side of the mirror.

When using the microscope objective lens to accompany the distance, you should rotate the dust control mountain coke wheel and slowly turn the focus down from the wild brother stupid.

a.That's right. b.Mistake.

Correct answer: B

The specific steps are as follows:

1. Find the approximate working distance; Install the autofocus camera, produce the picture normally, open the camera menu,; Adjust the microscope to the maximum magnification, and then adjust the focusing handwheel to make the image clear;

Second, correcting the posterior focus; Adjust the microscope to the minimum magnification, open the camera menu, and select Auto Pair; After the focus is completed, select MF and fix the back focus (note that only change the poly;

3. Correct the working distance; The problem of parfocal microscope is one of the major problems in the industry, but with an autofocus camera, this problem can be easily solved.

1.Place the prepared slide specimen on the stage, align the specimen with the round hole on the stage, and clamp the left and right sides of the slide with a fixing clip.

2.Turn the spinning disk and aim the low-magnification objective at the round hole on the stage.

3.Turn the coarse fixator until the low-magnification objective is closest to the slide specimen (the slide cannot be touched).

4.Observe from the eyepiece and adjust the angle of the mirror and the size of the aperture so that the brightness in the field of view is uniform and moderate.

5.Practice opening both eyes at the same time, looking through the eyepiece with your left eyepiece, slowly turning the coarse adjustment fixer, and then turning the fine adjustment fixation when you see the target.

until you can see clearly.

6.If you want to change the high-magnification objective, you must first move the part you want to magnify to the field of view**, and then change the soft-motion rotating disk to the high-magnification objective.

At this time, do not rotate the coarse adjustment retainer, just gently turn the fine adjustment retainer until it is clear).

1. Adjust the brightness: from dark to bright, you can use a large aperture, a concave mirror, and adjust the angle of the reflector.

2 Secure the slide in place on the stage.

3 The low-magnification objective lens is aligned with the clear hole, and the lens barrel is adjusted from top to bottom using a coarse collimation spiral, so that the eyes are on the side to avoid the objective lens from touching the slide and damaging the lens and crushing the slide.

4 The left eye observes the changes in the visual field through the eyepiece, and at the same time adjusts the coarse collimation spiral so that the lens barrel slowly moves up until the field of vision is clear.

5 If there is no object to be observed in the field of view, you can move the film, the principle is to go up and down, and to go left and right.

6 If it is not clear enough, it can be further adjusted with a fine collimation spiral.

7 If you need to observe under a high-magnification objective, you can turn the converter to change the objective. If the visual field is dark, it can be adjusted by method 1; If it is not clear enough, it can be adjusted by 6 method, but not by 4 method.

8 After use, adjust the adapter so that the empty lens hole is facing the clear hole, adjust the lens barrel to the lowest level, and then put it into the lens case.

Production of temporary films.

1. Drop a drop of water on the ** of the glass slide, put the material into it with tweezers, cover the coverslip and use absorbent paper to absorb the surrounding water and wipe it clean before putting it on the stage for observation.

2 If there are air bubbles, you can use a dropper to add water on one side of the coverslip and use absorbent paper to suction the air bubbles on the other side.

3 The stage must be kept level to avoid clean water flowing out of the stage and polluting the stage.

The quasi-focus knob on the microscope for adjusting the focal length has two thicknesses. When observing with a microscope, you should first turn the coarse focus knob clockwise to lower the lens barrel to 2 mm away from the slide, and then turn the coarse focus knob counterclockwise to slowly lift the lens barrel to look for the object. If the image of the object is not clear, turn the fine focus knob clockwise or counterclockwise to make the image clearer.

Therefore, when using a microscope to observe, adjust the quasi-focus knob to lift or lower the lens barrel, depending on the actual need, not necessarily whether to lift or lower.

If the problem is incomplete, it should be appropriately adjusted to the clarity of the object.

Because the magnification of the low magnification lens is small, the number of cells that can be observed in the field of view is large, but the cell structure can be carefully observed, so it is suitable to look for cells of the genus to be observed, rather than looking closely.

Because of the small magnification, the number of cells that can be observed in the field of view is small, and the field of view is slightly dark, so you can carefully observe the structure inside the cells, although it is suitable for observing cells, but it is difficult to find.

Due to the change of lens, the original adjusted focal length is not suitable for the new lens, so it is necessary to readjust the focal length.

In summary, the adjustment should be made from low magnification to high magnification and the focus must be adjusted.

1.Start by confirming that the thickness of the slide and coverslip is standard and make sure that it is placed face up.

2.Make sure that this microscope objective lens series is compatible.

3.Always focus with a low magnification lens first, and then convert the high-magnification objective, pay attention to observe whether the high-magnification objective lens and the specimen are rubbed, if the friction indicates that the thickness of the specimen and coverslip does not meet the standard or the specimen is reversed or the objective lens is not a matching series, the direct conversion should be stopped.

4.After ensuring that the glass slide, coverslip, and objective lens (different magnifications) are standard matching series, you can directly drop the lens oil to convert the oil lens after the low magnification is focused and use a thin spiral to slightly adjust it to observe**When changing, you should also pay attention to whether there is friction between the specimen and the objective).