What are the common aseptic procedures in life 30

1. Clean environment

Half an hour before the aseptic technique operation, it is necessary to stop cleaning the floor and other work to avoid unnecessary crowd flow and reduce the movement of personnel to reduce the dust in the indoor air. Prevent dust from flying. **The room is disinfected once a day with ultraviolet irradiation for 20 to 30 minutes, and the disinfection time can also be extended appropriately.

2. Aseptic operation

Dress neatly and neatly. Hats should cover all hair, masks should cover nose and mouth, nails should be trimmed, and hands should be washed. Wear sterile gowns and gloves if necessary.

3. Item management

Sterile articles and non-sterile articles should be placed separately, sterile articles should not be exposed to air, must be stored in a sterile bag or sterile container, once the sterile goods are used, they must be aseptically treated before they can be used, and the items taken out of the sterile container, although not used, can not be put back into the sterile container.

4. Sterile items

Sterile items must be stored in a sterile bag or sterile container, the sterile bag should indicate the name of the sterile, the date of disinfection and sterilization, the validity period of one week is appropriate, and the order of the date is discharged, so that it can be taken and placed in a fixed place. Sterile packs can be stored for 7 days without contamination and should be re-sterilized after expiration. Once the sterile goods have been used or expired, they should be re-sterilized when they are wet.

Principles of aseptic operation:

1. The environment should be clean, and half an hour before aseptic operation, the cleaning of the ground and other work must be stopped. Avoid unnecessary crowd movements and prevent dust from flying. **The room should be disinfected with ultraviolet light once a day.

2. When performing aseptic operation, the sterile and non-sterile areas of the article must be clarified.

3. Before performing aseptic operation, wear a hat and mask, wash your hands, dry your hands, and pay attention to air and environmental cleanliness.

4. To clamp sterile items, sterile forceps must be used. When performing aseptic operation in the aseptic operation room, all unsterilized hands and arms should not directly touch sterile items or take materials beyond the sterile area.

5. Sterile items must be kept in a sterile bag or sterilization container, and should not be exposed to air for too long. Sterile and non-sterile materials should be placed separately. Once opened, a sterile bag cannot be considered absolutely sterile and should be used as soon as possible.

Sterile items that have been removed cannot be put back into the sterile container even if they have not been used.

6. The sterile bag should be placed in the fixed cabinet in the order of the disinfection date, and kept clean and dry, separated from the non-sterilized bag, and frequently check whether the sterile bag or container is expired and whether the materials used in it are appropriate.

7. Sterile saline and alcohol, Xinjieer cotton ball tank should be disinfected once a week, and the dressings in the container, such as dry cotton balls, gauze blocks, etc., should not be overfilled, so as not to be contaminated outside the container when taking it.

The above content refers to: Encyclopedia - Aseptic Operation.

Aseptic technology is a series of operation techniques to keep sterile goods and sterile areas from being contaminated and prevent pathogenic microorganisms from invading the human body during medical care operations.

Aseptic technology is an important and basic technology for the prevention of nosocomial infection, and medical staff must master it correctly and skillfully, and strictly abide by the operating procedures in the technical operation to ensure the safety of patients and prevent the occurrence of hospital-source infection.

Principles of sterile storage:

1. Sterile items must be placed separately from non-sterile items.

2. Sterile items should not be exposed to air and should be stored in sterile bags or sterile containers. The name of the sterile bag and the date of sterilization should be marked on the outside of the sterile bag, and it should be discharged in the order of expiration date.

3. Regularly check the sterilization date and the preservation of sterile items. Sterile bags are generally stored for 7 days, and should be re-sterilized after expiration or moisture.

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The so-called aseptic operation refers to the space of the inoculation operation, the utensils and tools used, the clothing and hands of the operation, and the whole inoculation operation process of ensuring that the inoculation material is not contaminated with miscellaneous bacteria, and it is also one of the important technical links in the isolation and expansion of strains. Here's how it works:

1) Before inoculation, the inoculum to be inoculated must be cooled to a suitable temperature (28 30) to prevent high temperature inoculation of heat dead bacteria. At the same time, the inoculation box (room) should be cleaned and disinfected, and the inoculation tools should be prepared.

2) Move the sterilized and cooled inoculum (test tubes, bottles, bags) and various necessary items into the inoculation box (room) in advance, fumigate with drugs, and sterilize with ultraviolet lamps for 20 30 minutes or sterilize with an environmental sterilizer for 30 minutes, the effect will be better.

3) Vaccinators should wear sterile work clothes, work shoes, masks and work hats before the operation, and change into a set of clean clothes if they do not have them. Then disinfect the surface with a new clean solution solution, wash hands with disinfectant at the same time, and then bring the bacteria or isolate into the inoculation room (box).

4) Before the inoculation operation, wipe your hands, the surface of the bacterial culture container, the work surface and the inoculation tools with a 70% alcohol cotton ball, and then light the alcohol lamp to start the inoculation operation. When operating, it is necessary to concentrate on thinking, be strict and serious, and act lightly to minimize air flow.

5) After vaccination, remove all items in time and clean up. For continuous use, it must be re-sterilized and fully sterilized.

The technique used to prevent microorganisms from entering human tissues or other aseptic areas is called aseptic processing.

It is to avoid the influence of bacteria as much as possible during the experimental operation.

The concepts of sterility that should be followed in the operation of aseptic technology are: aseptic technique, sterile items, sterile area, non-sterile area, relative sterile area and contaminated items.

1. Aseptic technique refers to the operation technology and management methods that prevent all microorganisms from invading the body and keep sterile goods and sterile areas from being contaminated in the process of performing medical and nursing technology.

2. Aseptic supply After physical or chemical sterilization, the uncontaminated goods are called sterile articles.

3. Aseptic area The area that has been sterilized and is not contaminated is called the sterile area.

4. Non-aseptic area (non-aseptic area) Unsterilized or contaminated items or areas after sterilization are called non-sterile items or areas.

5. Relative aseptic area The relative aseptic area refers to the sterile item once it is taken out of the sterile container, it is considered to be relatively sterile and cannot be put back. The edge of the sterile zone is 3cm inward, which is the relative sterile zone.

6. InfectantContaminated goods refer to items that have not been sterilized, or are contaminated after sterilization.

Answer]: a. Aseptic operation method refers to an operation method or technology that uses or controls the world to control certain conditions in the whole operation process to avoid microbial contamination and return to the product.

1. Keep sterile items and sterile areas from being contaminated, so that the sterilized items remain sterile.

2. Prevent all microorganisms from invading the body or spreading to others.

Evaluation: 1. The operating environment is clean, spacious, bright, and regularly disinfected;

2. The operating table is clean and dry, and the layout of the items is reasonable;

3. Half an hour before aseptic operation, the cleaning work should be stopped to reduce the movement of personnel and avoid dust.

1.What is aseptic operation?

Aseptic manipulation refers to experiments and operations performed under completely sterile conditions. In order to avoid contamination by microorganisms such as bacteria and viruses, specific measures must be taken to keep the laboratory in a sterile state.

2.Precautions for aseptic handling.

1) Wear protective equipment such as clean protective clothing, hats, masks, gloves, etc.

2) Hands must be washed thoroughly and disinfected with 96% ethanol before operation.

3) Use sterile petri dishes and sterile instruments and equipment to ensure that the operation method is fast and accurate during inoculation.

4) Pay attention to keep the environment clean and tidy during operation, and do not let harmful bacteria enter.

5) After the operation, handle and disinfect all items correctly, keep them clean and prevent cross-contamination.

3.Aseptic procedure.

1) Clean and disinfect the test bench according to the experimental requirements, and irradiate it with ultraviolet lamp for 15 minutes to disinfect and eliminate bacterial contamination.

2) Place the required instruments and equipment on a sterilized laboratory bench.

3) Put all the required media, strains, reagents, etc. into the uncovered Zheng fungus utensils used in the laboratory.

4) Wear protective clothing and gloves, and wash hands thoroughly before handling.

5) Under aseptic operation, carry out experimental operations to minimize the contact and contamination of the medium and sample.

6) After the operation, a series of cleaning and disinfection processes should be carried out, the sterile operation room should be cleaned, and the ultraviolet lamp should be turned off.

4.Aseptically operated utensils.

1) Sterile Petri dishes.

2) Sterile pipette.

3) Sterile well plates.

4) Potted Petri dishes.

5) Aseptic plate homogenizer.

6) Cell culture incubator.

7) Ultraviolet disinfection lamp.

5.The level of the clean room.

Sterile laboratories are classified into different levels according to cleanliness, including:

1) Level I: Local High Clean Area (LAF).

2) Level II: Sedimentation clean bench.

3) Level III: Fully enclosed clean (ultra-clean) room with positive pressure air purification system.

6.Areas of application for aseptic operation.

Aseptic operation is widely used in biotechnology, pharmaceutical industry, food industry and other fields. Aseptic operation can effectively ensure the accuracy of the experiment and the reliability of the results.

7.Risks and precautions for aseptic procedures.

1) Microbial contamination: Strictly implement aseptic operation specifications to ensure that microorganisms will not enter the laboratory or operation area.

2) Physical hazards: Pay attention to the correct use of sterile utensils and equipment to prevent damage and injury to utensils and equipment.

3) Chemical hazards: pay attention to the nature and safe use of chemical reagents to prevent damage and injury of chemical reagents.

4) Damaged gloves: Change gloves frequently and pay attention to the condition of gloves to prevent contamination caused by broken gloves.

8.Epilogue.

Aseptic handling is an essential skill in the modern laboratory. Through rigorous standardization, clean equipment and standardized operation procedures, the accuracy of experimental results can be ensured, the success rate of experiments can be improved, and the contamination of bacteria and other microorganisms can be effectively avoided, so as to protect the health and safety of experimental workers.

The operating principles of aseptic technique are as follows:

1. First of all, aseptic operation should be carried out in a relatively sterile environment.

2. The operator must bring a mask, a hat, a sterile suit, and then wear a sterile glove, at this time you can contact the sterile goods, and then use the sterile goods for aseptic operation. Therefore, the basic operating principle of aseptic technology is to strictly carry out aseptic operation, and no fungal matter can be touched during the operation, otherwise the operation will fail.