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A micromicroscope microtome is a tool used to prepare thin sections for microscopic observation. The following are the steps to use a micromicroscope microtome in general:
Prepare the sample: Prepare a small piece of the sample that needs to be observed, such as plant tissue, insect parts, or other materials. Make sure the sample is stable and can be fixed on the microtome.
Adjust the microtome: Depending on the size and shape of the sample, adjust the microtome's gripping device in order to hold the sample in place.
Adjust the blades: Make sure the blades on the slicer are sharp and in place. The angle and depth of the blade may need to be adjusted to ensure the quality of the slices.
Sectioning the sample: Gently place the sample on the blade of the microtome and secure it using a gripping device. Then, slowly push the microtome to allow the blade to cut along the sample, preparing the thin section. Take care to keep your hands steady to avoid slices that are too thick or uneven.
Collect the sections: Place the prepared thin sections in a microscope slide or other suitable container for observation. Sections can be stained or otherwise processed prior to light microscopy.
Note that different micromicroscope slicers may have different designs and usages. Therefore, it is best to look for the instruction manual for that specific device or get more detailed guidance from a ** vendor.
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Just like a potato knife, make a thin slice so that you can put it under a microscope to observe.
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Slice things under a microscope.
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Use the switch on the right side of the host to turn on the power supply, use the box temperature setting button to set the temperature of the slicer to the desired temperature of the slices, when the temperature is set, the temperature display window displays the set temperature, and the actual temperature is displayed after the setting stops for 5 seconds. As long as you press the box temperature setting button, you can check the set temperature at any time. The sample head temperature is set in the same way.
During sectioning, the sample is placed on the sample holder, fixed with embedding medium, placed on a cold table for freezing, and flattened with a heat exchanger before it is about to be completely cooled. Put the cooled sample on the sample head, set the sample head temperature, use the sample fast-forward button to move the sample closer to the knife edge, adjust the plane to be cut, use the slow forward button to start trimming, and then put down the anti-coil plate slice after repair, if necessary, adjust the upper and lower positions of the anti-coil plate, so that the cut out sample smoothly enters the slit between the anti-coil plate and the blade, and stain and observe after taking it out. When the machine is stopped, the glass window should be opened, and before turning on the machine again, it should be checked whether there is water in the slicer, if there is one, it should be dried before turning on the machine, and the partition should be taken out to check whether there is water at the bottom of the slicer box, if there is a need to pull out the plug at the bottom, the water will be removed, and the machine should be turned on after drying.
It is forbidden to randomly connect and pull wires at will to prevent water from splashing on the power supply.
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The first is to place the mirror with your right hand and the mirror holder with your left hand to keep the mirror body upright. The table top should be clean and stable, and choose a window or well-lit place. The monoculars are generally placed on the left side, 3 or 4 cm away from the edge of the table.
Check whether there is anything wrong with the microscope and whether it is clean, and the mechanical part of the mirror body can be wiped with a clean stool and a soft cloth.
The tube is raised to 1 2 cm from the stage, and the low magnification lens is aligned with the clear hole. Adjust the aperture and reflector, use a flat mirror when the light is strong, use a concave mirror when the light is weak, and turn the mirror with both hands.
Place the slide on the stage, noting that the side with the coverslip must be facing up. Secure the slide with a spring clip.
To focus, slowly lower the barrel by rotating the coarse focus knob and look carefully from the side until the objective lens is close to the slide specimen.
If a monocular microscope is used, the two eyes are opened from the thick middle, the left eye is used to observe the specimen, the right eye is observed to observe the recording and drawing, and the left hand adjusts the focus to make the object clear and move the specimen field of view. Right-handed recording and drawing.
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