What are the methods of microbial culture, what equipment is commonly used in microbial culture tech

That's too many, and there must be hundreds.

Mainly: ultra-clean workbench, constant temperature incubator, anaerobic incubator, refrigerator and low temperature freezer, autoclave, biological safety cabinet, shaker, microscope, constant temperature water bath, constant temperature drying oven, acidity meter, spectrophotometer, muffle furnace, high-speed tissue masher, multi-purpose shaker, standard sieve, a large number of various glass instruments, a large number of various biochemical reagents and chemical preparations.

Anyway, the instruments and equipment in general chemical laboratories and chemical analysis rooms can be used in microbial culture.

The commonly used equipment for microbial culture technology includes: autoclave, which is used for sterilization; Incubators and shakers for culturing microorganisms.

The culture technology and application of microorganisms include aerobic culture and anaerobic culture.

The application is the continuous discovery and extensive application of various antibiotics, the study of bacterial cell and virus morphology has reached the level of submicroscopic structure, so as to further understand their activity rules, further elucidate the nature, composition and mechanism of bacterial endotoxins and exotoxins, significantly improve the isolation and culture technology, and greatly improve the positive rate of isolating Campylobacterium or bacteroides from patient specimens.

Aerobic culture is also called aerobic culture. That is to say, when this kind of microorganism is cultivated, it needs to be added with oxygen, otherwise it will not grow well. In the laboratory, bevel culture is obtained from the outside world by means of a cotton plug. Triangular flask.

Liquid culture is mostly shaken by shaking on a shaker to continuously feed air from the outside into the bottle.

Microbial culture techniques

Anaerobic culture is also called anaerobic culture. These microorganisms do not require oxygen to participate in the cultivation. One of the most important aspects of the culture of anaerobic microorganisms is the removal of the medium.

in oxygen. Research and development of immunogenicity.

Well, the smallest new microorganisms, the development of specific, sensitive, simple, rapid microbiology.

Diagnostic methods and techniques.

<> configure different concentrations of nutrient solution diluents; Extract the dilution (1 ml) and add it to a tube filled with 9 ml of sterile water, and so on to make dilutions of 1% and other different concentrations; Pour the solution into Petri dishes and mark the concentration on each Petri dish; Inoculate the nutrient solution at each concentration, then gently shake it to the Petri dish of the seeds, and then store it in an incubator (37°C).

Liquid inoculation.

Washing the bacteria from the solid medium of Zhonghai Biotechnology and pouring them into the liquid culture medium, or from the liquid culture, using a pipette to connect the bacteria into the liquid culture medium, or moving the bacteria from the liquid culture to the solid culture medium, can be called liquid inoculation.

Puncture inoculation. This method is often used to preserve anaerobic strains or to study the dynamics of microorganisms. When needle vaccination is done, the inoculation tool used is the inoculation needle. The medium used is generally a semi-solid medium.

It is done by dipping a small amount of the inoculation needle into a small number of strains and making a straight puncture along the center of the semi-solid medium to the bottom of the tube, and if a bacterium has flagella and can move, it can grow around the puncture line.

Live inoculation. Live inoculation is a method used specifically to culture viruses or other pathogenic microorganisms because viruses must be inoculated into living organisms in order to grow and reproduce. The living organism used can be a whole animal; It can also be an ex vivo biopsy, such as a monkey kidney; It can also be a developed chicken embryo. Inoculation can be done by injection or mixture feeding.

Pour mixed inoculation. In this method, the microorganisms to be received are first put into the petri dish of Zhonghai Biology, and then poured into the solid medium cooled to about 45 °C, and quickly and gently shaken well, so that the bacterial solution can achieve the purpose of dilution. After the plate is solidified, it can be incubated at the right temperature to grow a single microbial colony.

Scribing inoculation. This is the most commonly used method of vaccination. That is, the surface of the solid medium can be moved back and forth in a straight line, and the effect of inoculation can be achieved. Commonly used inoculation tools include inoculation loops, inoculation needles, etc. This method is commonly used in inclined inoculation and plate marking.

Coating inoculation. It is slightly different from pouring mixed inoculation, that is, first pour the plate, let it solidify, and then pour the bacterial solution into the plate, and quickly use the coating rod to coat back and forth on the surface, so that the bacterial liquid is evenly distributed, and a single microbial colony can grow.

In 1905, the German microbiologist Koch was awarded the Nobel Prize for his significant achievements in the study of Mycobacterium tuberculosis and tuberculin. He invented the solid medium and used it to isolate and culture bacteria, creating a pure culture method for bacteria. He also improved the bacterial staining method to create favorable conditions for the study of bacterial morphology and structure.

Dutch scientist Prof. E. C Hansen studies the yeast that ferments beer, creating a pure culture method for single cells. Later, some people used the pure culture method to select the appropriate yeast for the industrial production of beer, which opened the way for the application of the pure culture method in industrial fermentation. Haas, a student of Hanson, found that the solid medium made of gelatin used at the time was prone to liquefaction and difficult to use.

Haas's wife suggested using agar instead of gelatin, and the results were good. This agar medium was adopted by later generations and is still in use today. Later, a Petri dish was created for the separation of microbial plates.

Since then, more and more microorganisms have been isolated and new microorganisms have been discovered. In this way, microorganisms that can be used for industrial use are increasingly abundant, and a new force of microorganisms has sprung up.