What are the commonly used instruments and equipment in microbiology laboratories?

The commonly used instruments and equipment in the microbiology laboratory include anaerobic incubators, constant temperature incubators, dry heat sterilization boxes, autoclaves, and ultra-clean workbenches.

1. Anaerobic incubator.

Anaerobic incubators are also known as anaerobic workstations or anaerobic glove boxes. An anaerobic incubator is a special device for bacterial cultivation and operation in an anaerobic environment. It can provide strict anaerobic state, constant temperature incubation conditions and has a systematic and scientific working area.

2. Constant temperature incubator.

Thermostatic incubator is a general term for a type of thermostatic chamber. It is widely used in medical and health care, pharmaceutical industry, and biochemistry.

Scientific research departments such as industrial production and agricultural science are mainly responsible for cultivating various microorganisms or organisms such as tissues and cells.

3. Dry heat sterilization box.

The dry heat sterilization box adopts the dry heat sterilization method, which refers to the method of sterilization with high temperature dry hot air. This method is suitable for glass and metal products that are resistant to high temperatures and do not allow damp heat.

Sterilization of gas-penetrating oils and fats (such as oil-based ointment mechanisms, oils for injection, etc.) and high-temperature-resistant powder chemicals, suitable for high-temperature resistant glassware, porcelain, and glass syringes.

It is not suitable for the sterilization of rubber, plastic and most drugs.

4. Autoclave.

Autoclave is a device that uses saturated pressure steam to quickly and reliably sterilize items, which is suitable for medical and health, scientific research, agriculture and other units. Autoclaves are divided into portable autoclaves, vertical autoclaves, and horizontal autoclaves according to their styles and sizes. It can be used for medical devices, dressings, glassware, solution media.

and so on.

5. Ultra-clean workbench.

The ultra-clean workbench, also known as the purification workbench, is to adapt to modern industry, optoelectronic industry, and biopharmaceutical.

and scientific research, testing and other fields for the cleanliness of local work areas designed.

It draws air through a fan into the pre-filter and through a hydrostatic pressure box.

Enter the HEPA filter.

Filtration, the filtered air is sent out in the state of vertical or horizontal airflow, so that the operation area can reach the 100-level cleanliness and ensure the requirements of environmental cleanliness for production.

1. Ultra-low temperature freezer: cryopreservation of vaccines, strains, biological samples, etc.

2. Liquid nitrogen tank: long-term active preservation of vaccines, bacteria and viruses, and cells.

3. Drug refrigerator: used for refrigerated storage of drugs, reagents, biological products and vaccines.

Commonly used instruments in microbiology laboratories.

1.Clean room and clean bench.

2.Incubator: biochemical incubator, ordinary incubator, constant temperature incubator, anaerobic incubator.

3.Constant temperature drying oven.

4.Autoclave.

5.Balances: Electronic balances (with an accuracy of 1 thousandth or 1 part per 10,000) or analytical balances.

6.Microscope:

7.Spectrophotometer (to determine the amount of bacterial growth).

8.Acidity meter.

9.Thermostatic water bath.

10.Refrigerator: ordinary refrigerator, low temperature refrigerator.

11.Centrifuge.

12.Homogenizer.

13.Shake the culture shaker.

14.Anaerobic culture equipment.

15.Water purification equipment.

16.Ventilation equipment.

17.Electric stove or microwave oven.

18.Magnetic stirrer.

19.Hemocytometer, eyepiece micrometer, mirror table micrometer, hand counter.

20.Colony counter.

21.Commonly used glass instruments: (various models) test tubes, petri dishes, triangular bottles, reagent bottles, pipettes, rubber tip droppers, glass slides, coverslips, funnels, beakers, graduated cylinders, tweezers, inoculation loops, alcohol lamps, glass rods, etc.

22.Commonly used medicines: (Note: according to the needs of the experiment).

23.Multi-** teaching equipment.

Microbial purification, separation, identification, and proliferation in a sterile environment.

Main test items: The SIGN-Microbiology Laboratory is divided into conventional bacteria, pathogenic bacteria testing, virus, probiotics, parasite testing, etc. according to the risk degree of the test object. Routine bacterial testing usually includes:

total number of colonies, E. coli, coliforms, etc.; Pathogenic bacteria include: Salmonella, Staphylococcus aureus, Shigella, etc.

That's too many, and there must be hundreds.

Mainly: ultra-clean workbench, constant temperature incubator, anaerobic incubator, refrigerator and low temperature freezer, autoclave, biological safety cabinet, shaker, microscope, constant temperature water bath, constant temperature drying oven, acidity meter, spectrophotometer, muffle furnace, high-speed tissue masher, multi-purpose shaker, standard sieve, a large number of various glass instruments, a large number of various biochemical reagents and chemical preparations.

Anyway, the instruments and equipment in general chemical laboratories and chemical analysis rooms can be used in microbial culture.

The commonly used equipment for microbial culture technology includes: autoclave, which is used for sterilization; Incubators and shakers for culturing microorganisms.

The commonly used instruments and equipment in the microbiology laboratory include ultra-clean workbenches, incubators, balances, microbial homogenizers, colony counters, microwave ovens, electric stoves, autoclaves, pipettes, low-temperature refrigerators, biological safety cabinets, etc

1. Ultra-clean workbench.

The culture of microorganisms is aseptically cultured in a specific medium, so the aseptic culture must provide a sterile working environment on a clean bench.

2. Incubator.

There are many types of incubators, and their role is to provide a suitable environment for the growth of microorganisms. The biochemical incubator can only control the temperature and can be used as a plate culture for general bacteria; The mold incubator can control the temperature and humidity and can be used as a mold culture; CO2 incubators are suitable for the cultivation of anaerobic microorganisms.

3. Balance <>

Balances are used to accurately weigh a wide range of reagents. Electronic balances are commonly used in laboratories, and there are different levels of accuracy depending on the level.

4. Microbial homogenizer.

It is used to extract bacteria from solid samples. The preparation of microbial test samples with microbial homogenizer has the characteristics of no pollution, no damage, no heating, no sterilization treatment, no need to wash utensils, etc., which is a more convenient instrument to use in microbial experiments.

5. Colony counter.

A colony counter assists the operator in counting the number of colonies. Accurately obtain the number of colonies by magnifying, taking pictures, counting, etc. Some high-performance colony counters can also be connected to a PC for automatic counting.

6. Microwave oven electric stove.

It is used for rapid heating of solutions, heating and dissolving of microbial solid medium.

7. Autoclave.

Most of the experimental items, reagents, and culture media used in microbiology should be strictly sterilized and sterilized. Sterilizers are also available in different sizes, some are manual and some are fully automatic. Users need to choose according to their own needs.

8. a pipette.

Liquid measuring devices are used to accurately measure all kinds of liquids. Common liquid measuring devices include graduated cylinders, pipettes, microdispensers, graduated test tubes, and beakers.

9. Low-temperature refrigerator.

Refrigerators are indispensable for storing reagents and samples in the laboratory. Some of the reagents used in microbiology experiments are required to be stored at 4 degrees, and some are required to be stored at minus 20 degrees.

10. Biological safety cabinet.

Some of the reagents and sample microorganisms involved in microbiological experiments are toxic and harmful to operators. In order to prevent the spread of harmful suspended particles and aerosols, the biological safety cabinet can be used to provide safety protection for operators, samples and cross-contamination between samples and the environment.

The microbiology laboratory is mainly responsible for the detection of various microbial items, including the total number of colonies, the type of colonies, pathogenic coocci, etc. According to different classifications, microbiology laboratories have different safety requirements and use requirements, so microbiology laboratories are different from the construction concept of general laboratory (purification) projects.

The basic requirements for the construction of the microbial testing laboratory are that the surface of the wall must be smooth, waterproof and corrosion-resistant, the ground must be leak-free, smooth but not slippery, and the furnishings of the instruments and equipment are simple, easy to clean, easy to disinfect and clean, so that the experimental environment can be kept (clean) and sterile as much as possible. The basic furniture of the microbiological testing laboratory includes: laboratory bench, ultra-clean workbench, biological safety cabinet, etc.

In order to facilitate cleaning and cleaning, it is recommended to choose an isolated ** laboratory bench, which has a functional area dry and wet isolation module, which can be moisture-proof and resistant to Li Wei corrosion, and the dry and wet areas are separated, making the experiment safer. The better isolated ** test bench should also be equipped with a full-station water and electricity supply function to flexibly meet the experimental needs; In order to facilitate access to reagents, you can also make full use of the space to design the stereo reagent storage space.

In addition, in order to provide a better experimental environment for microbial detection experiments and ensure the accuracy of experimental results, the laboratory has extremely high requirements for the cleanliness of the environment.

Conventional microbiology laboratory configuration: 1, incubator: 3 sets (bacteria, molds, pathogenic bacteria) 2, electronic scale: 1 set.

3. Homogenizer: 1 set.

4. Colony counter: 1 set.

5. Microwave oven: 1 set.

6. Autoclave: 2 sets.

7. Dispenser: 2 pcs.

8. Refrigerator: 2 sets.

9. Alcohol lamp: 10 pcs.

10. Test tube rack: 20 30 pcs.

11,500ml triangular bottles: 50 100 pcs.

12. Graduated cylinder: (2 pcs for 250ml, 2 pcs for 500ml, 2 pcs for 1000ml).

13. Glass test tubes: 500 1000 pieces.

14. Sterilization straw: 1ml, 10ml each according to the daily dosage 15, cool drying rack: 2.

16. Scissors, tweezers: 40 to 60 pcs.

17. Absorbent cotton, gauze.

18, test tube basket: 10 20 pcs.

19. Sterile sampling and weighing sample bag: according to the daily dosage.