To establish a microbiology laboratory, please point out with your fingers

I only said the following main equipment (there are too many small things, so I won't talk about it): aseptic operation table, 3 incubators (bacteria, mold, and the control bacteria of the golden Portuguese green purulent colon have different culture temperatures), sterilization equipment, a buffer room if the sterile room is not for injection samples, and there are two changing rooms (because you have to change sterile clothes), ultraviolet of course, air requirements of 10,000 (do you think air filtration is necessary), and air monitoring equipment (suspended particles, planktonic bacteria).

Don't talk about other small pieces, too much, just buy a copy of the "Chinese Pharmacopoeia" if you go through the process!

If it is only for the detection of the formulated microbial concentration, you can directly buy the corresponding nutritional reagent bottle, which is convenient and fast, does not need a sterile operation room, and the cost is too large, just buy an ultra-clean workbench directly, which has an ultraviolet sterilization lamp and an incubator.

2: There should be a sterile table, pipette gun, high-speed centrifuge, petri dish, coverslips, glass slides, alcohol lamps, etc.

3: I don't know.

4: In principle, 2 buffer rooms are better, and it is better to install ultraviolet lamps.

5: The sterile room can be air-conditioned, and the air filtration system must be used, with an area of at least 50 square meters.

I majored in biotechnology in college, and I have been exposed to these aseptic operations, and I am giving basic advice, which may not be very professional, and it is best for the landlord to find a professor who does microbial research to give you an answer.

You can build this kind of laboratory yourself, and it is better to use the money to do it for SGS for a long time.

The depreciation of the purchased equipment, plus the salaries of the personnel, plus the rent of the premises plus the operating costs (electricity, reagents, etc.) is enough for you to go to SGS twice a day.

It's okay to set up a laboratory internal control, but there is no need to be so troublesome, and since it is an export, the customer will definitely ask for a third-party test report, and then a third party will have to do it, but I don't know how many items you need to test in SGS for 700 yuan? If you can, leave me a message, I am a salesman of SGS, and I have the right to discount.

To add: a high-temperature sterilization pot, an incubator, a microbiological analyzer if conditions permit, various reagents.

I don't know if the landlord has heard of the French bio Mérieux, for what you said about question 3, about screening and identification, you can go to the food partner network, where there are GB4789-2008 and 22429-2008 national standards**,

Commonly done microbial experiments are: drug susceptibility test: mainly by measuring the scope of the bacteriostatic zone to determine the efficacy of the drug.

Hemagglutination assay: The antibody titer of the virus is determined by measuring the concentration of the virus in response to red blood cells. pcr：

Bacteria mainly determine the species of their bacteria by 16srRNA. Neutralization experiment: The dilution concentration of the virus is mainly determined.

Gram stain: Determine if it is Gram-positive or negative. Wright's staining:

Mainly staining for bacteria.

A microbiology laboratory is a place where microbial research is conducted. According to the safety requirements and use requirements of the microbiology laboratory, it should be different from the general laboratory engineering or purification engineering. The laboratories mainly used in the research of microbiology, biomedicine, biochemistry, animal cress experiments, genetic recombination and biological products are collectively referred to as the Biosafety Shield.

The biosafety laboratory is composed of the main functional laboratory, other laboratories and auxiliary functional rooms. Biosafety laboratories must ensure the safety of people, the environment, waste and samples, and operate safely for a long time, while also providing a comfortable and good working environment for laboratory staff.

A microbiology laboratory is a place where microbial research is conducted. According to the safety requirements and use requirements of the microbiology laboratory, it should be different from the general laboratory engineering or purification engineering. Laboratories mainly used in microbiology, biomedicine, biochemistry, animal experiments, genetic recombination, and biological products are collectively referred to as biosafety laboratories.

Preparation room: The preparation room is used for the preparation of culture media and sample processing, etc. The room is equipped with reagent cabinets, counters for storing utensils or materials, laboratory benches, electric stoves, refrigerators, water and sewerage, power supplies, etc.

Sterilization room: The sterilization room is mainly used for the sterilization of culture medium and the sterilization of various utensils, and the room should be equipped with high-pressure steam sterilizer, oven and other sterilization equipment and facilities.

Sterile room: The sterile room, also known as the inoculation room, is a special laboratory for aseptic operations such as systematic inoculation and purification of strains. In microbial work, inoculation and transplantation of strains is a major operation, which is characterized by ensuring that the strains are pure, and preventing contamination by miscellaneous bacteria.

In the air of the general environment, due to the presence of a lot of dust and miscellaneous bacteria, it is easy to cause pollution, which greatly interferes with the inoculation work.

Washing room: The washing room is used for scrubbing utensils, etc. Pathogenic microorganisms are sometimes present due to microbial contamination of used utensils.

Therefore, if conditions permit, zui-good set up the washing chamber. The room should be equipped with heaters, steamers, basins and buckets for washing utensils, as well as various bottle brushes, decontamination powder, soap, washing powder, etc.

Purifying air conditioners: York, Carrier, McQuay, Tianjia, Iceberg.

Air shower room, transfer window: this is too much, it's hard to say.

Pressurized fan: Generally, box-type centrifugal fans are used, such as Kruger, Greenhank, and Yilida.

Access control, this is too much.

Laboratory sidestand, there are also a lot, better brands: fisher (super expensive), Beijing Mingyuan and other color steel plates: very much, I also say something good, Shanghai Xingtie, Suzhou Baishun PVC:

France Dejia, the United States Armstrong, France Gerflor, this is divided into import processes, the gap is very large.

Laboratory infrastructure materials** decide, you need a good one can be proposed directly to the company that does the laboratory, the materials are optional, different companies have their own brands, but the materials used are basically the same, there is no difference.

This series of facilities can be designed by an authoritative manufacturer, according to the actual layout of your laboratory, first design a floor plan, and repeatedly modify it with your requirements, and then have them build you to accept! We are built by Shenyang Tianma Group, which is very good, you can check it!

What kind of company are you, can I get to know you?

Here's my microbiology lab equipment, and it's very simple to describe It's hopefully helpful to you.

List of microbiology laboratory instruments and equipment.

1. 1-2 test benches.

2. 1 fume hood.

3. 1 aseptic operation table (airtech).

Alcohol lamps 2pcs.

2 inoculation needles.

2 coating sticks.

5. 1 autoclave (Hirayama: HVE-50 automatic autoclave) is in parentheses for reference.

7. Refrigerator. 8. Microscope.

Glass slide coverslip 9, constant temperature water bath.

10. Electronic balance.

11. a graduated cylinder.

1000ml 2pcs.

500ml 2pcs.

100ml 2pcs.

12. a beaker.

About 500ml of 3 pcs.

13. Test tube + test tube stopper.

15*150 20pcs.

18*180 10pcs.

14. an Erlenmeyer flask.

About 5 pieces of 100ml.

About 20 pieces of 250ml.

500ml or so.

15. a volumetric flask.

500ml or so.

16. Pipette or 1-l0ml pipette + pipette tip.

1ml of 10pcs.

5ml of 10pcs.

17. a transparent small-mouth bottle.

18. Brown small-mouth bottle.

19. a test tube rack.

20. a glass rod.

21. Petri dishes about 50.

22. a parafilm.

23. Stainless steel surgical scissors.

24. Stainless steel medicine spoon and tweezers.

25. Induction cooker or electric heating stove.

26. There are about 3 watering cans, rubber head droppers, and ear suction balls.

27. pH meter or PH test strip.

28, shaker.

This bai

Some are definitely needed, and that's exactly what we used to do in the lab:

0.Microbial DAO cultivation equipment version, such as fermenters and the like.

1. A test bench.

Right 1-2 units.

2. 1 fume hood.

3. 1 aseptic operation table (airtech).

Alcohol lamps 2pcs.

2 inoculation needles.

2 coating sticks.

5. 1 autoclave (Hirayama: HVE-50 automatic autoclave) is in parentheses for reference.

7. Refrigerator. 8. Microscope.

Glass slide coverslip 9, constant temperature water bath.

10. Electronic balance.

11. a graduated cylinder.

BAI needs to build a P2 clean room:

P2 Experimental DU Chamber:

1. Compliant.

Requirements for the construction of the ZhibSL-1 experimental DAO room.

2. The door of the laboratory is locked and can be closed automatically. The door of the laboratory should have a viewing window.

3. Have enough storage space to store items for easy use. There is also storage space for long-term use in the lab work area.

4. There should be conditions for storing personal clothes in the working area of the laboratory. 6. All buildings in the laboratory are equipped with autoclaves, and are inspected and verified on a regular basis to ensure that they meet the requirements.

7. Equipped with biological safety cabinets in the laboratory.

8. Set up eyewash facilities, and if necessary, there should be emergency sprinkler devices.

9. Consider mechanical ventilation, such as using windows for natural ventilation and insect screens. 10. There is reliable electricity** and emergency lighting. When necessary, important equipment such as incubators, biosafety cabinets, refrigerators, etc. should be used.

Power supply. 11. The exit of the laboratory is clearly recognizable in the dark.

Other equipment depends on the content of your experiment, and is generally not contaminated or disrupted by laboratory airflow control.

Research on antimicrobial materials.

I think if it is a powder or liquid DAO material, you don't need an internal grinder, if it is a fiber or solid block, you can buy a grinder or something like that, and the other is the main aseptic operation table (if you are familiar with aseptic operation and the requirements are not very strict), you can not do it), incubator or shaker, scale (electronic is the best), sterilizer, and a series of media materials, utensils, etc. I don't know how you are going to design this experiment, I personally think it is mainly a control experiment, but if the antibacterial material is not resistant to high temperatures, you have to find a more reasonable sterilization plan.