Who knows the specific operation steps of the microscope in biological experiments?

The difference is that light microscopy can only see certain cellular structures such as cell walls, chloroplasts, stained chromosomes, mitochondria, nucleus, etc., while electron microscopy can see the internal structure of organelles and smaller organelles like ribosomes. The microstructure of the cell is seen with a light microscope, and the submicrostructure can be seen with an electron microscope.

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Place the light --- loading --- lower the lens barrel --- lift the lens barrel--- and --- move the part to be observed to the field of view**--- turn the converter to the high magnification --- observe.

Take the mirror and place it, align the light, and observe.

1. Take and place the lens 1 Hold the mirror arm with your right hand and hold the mirror base with your left hand. 2 Place the microscope about 7 cm from the edge of the bench, slightly to the left. Attach the eyepiece and objective.

2. Turn the converter to align the low-magnification objective with the clear aperture (keep a distance of 2 cm between the front end of the objective and the stage).Aim a larger aperture at the clear aperture. One eye is in the eyepiece and the other eye is open.

Turn the mirror so that the light is reflected into the barrel through the aperture. Through the eyepiece, you can see a bright white circular field of vision. 3. Observation (under low magnification, put the slide specimen to be observed on the stage, press it with a tablet clamp, and the specimen should be facing the center of the light hole.)

Turn the coarse collimator spiral so that the lens barrel is slowly lowered until the objective lens is close to the slide specimen (at this point the eye must look at the objective).4. Focus one eye to look into the eyepiece, and at the same time rotate the coarse quasi-focus spiral in the counterclockwise direction, so that the lens barrel slowly rises until the object is clearly visible. Then turn the fine collimation spiral slightly to make the image of the object seen more clearly.

5. Observation under high magnification 6. Move the image of the object observed under low magnification to the ** of the field of view. 7. Turn the converter and observe with a high magnification lens, and gently turn the fine collimation spiral until you can see the image clearly.

1. Placement: The microscope should be placed in front of the body to the left, and the lens barrel should be placed in the direction of the front mirror arm and the rear direction.

2 pairs of light: use a low magnification lens, a large aperture (the light shield is adjusted upward); Look at the eyepiece and adjust the mirror at the same time; Brightens up the field of vision.

3. Put the film: The observation object should be facing the hole of the objective lens, and then adjust the focus after clamping the slide.

4. Focusing: first use a low magnification lens to find the object, first lower the lens barrel and then raise the lens barrel, when lowering the lens barrel, you should observe whether the film is pressed on the side, and when the lens barrel is raised, you should look for the object image in front of the eyepiece. Move the object image to the center of the field of view, and use a high magnification lens to observe and only adjust the fine focus spiral to make the object clear.

5. Observation: Open both eyes, observe with your left eye, and draw with your right eye. Thank you!

Precautions for use of the microscope and its use.

How to use an optical microscope.

The most used oil lens in biology is to learn how to identify the oil lens first, so as not to be confused with ordinary lenses and damage the lens. Usually the length of the oil lens is larger than that of the low and high magnification lenses, and the lower edge of the lens is generally engraved with a circle of black or white lines, and engraved with the words 100, or oil. Before using the oil lens, first use a low magnification lens to find the target and then drop oil to switch the oil lens, the front lens should be attached to the glass slide, make coarse adjustments when observing, fine-tune after seeing the target, and wipe the oil on the lens with a lens paper after use.

First, take the lens

Second, to the light

Through the eyepiece, you can see a white and bright field of vision.

3. Observation

1.Place the slide specimen to be observed on the stage and press it with a tablet clamp to face the light hole. Centre.

2.Turn the coarse collimation spiral first so that the lens barrel slowly lowers until the objective lens approaches.

Up to the slide specimen (look at the objective lens so that the objective lens does not touch the slide specimen).

3.The left eye looks into the eyepiece and turns the coarse procal spiral in the opposite direction so that the barrel slowly rises until the object is clearly visible. Then turn the fine collimation spiral slightly to make the image of the object seen more clearly.

4. After the experiment, wipe the surface of the microscope clean. Turn the converter to offset both objectives and slowly lower the barrel to the lowest point. Finally, put the microscope in the case and return it to its original place.

1.Use of microscopes.

1) The eyepiece and objective lens have a certain magnification = eyepiece x objective.

2) When moving the slide, the slide moves in the opposite direction to the object in the field of view.

3) The image in the field of vision and the object in the specimen are inverted. If the object is P, the actual object is D.

4) Rotating the coarse collimation spiral can make the lens barrel move up or down to a large extent; When the fine collimation spiral is rotated, the image can be adjusted more clearly.

1. Turn the low magnification lens to the bottom of the lens barrel in a straight line with the lens barrel.

2. Toggle the reflector and adjust it to the brightest field of view without shadows. The reflector has flat and concave sides, the light source is strong when the plane is used, the dark light is used concave, when the strong light is needed, the condenser is raised, and the aperture is magnified; When low light is required, lower the condenser or shrink the aperture appropriately.

3. Place the specimen to be observed on the stage, and turn the coarse adjuster to lower the lens barrel to the point where the objective lens is close to the specimen. While turning the coarse regulator, you must lean over the mirror and carefully observe the distance between the objective lens and the specimen.

4. The left eye is observed in the eyepiece, and the left hand is rotated to adjust the lens barrel slowly to adjust the focal length, so that the objects in the field of vision will stop when they are seen until the specimen is clear.