If you want to convert the 1500 factory into a PCR sequencing laboratory, how to design it?

The key to the overall design of a PCR sequencing laboratory is how to avoid contamination, and four areas are generally constructed: experimental preparation area, preparation area, amplification area and analysis area. **One-way flow in and out of the laboratory area can reduce the risk of cross-contamination, and in order to ensure that personnel flow in each area in one direction, it can be distinguished by wearing different colors of work clothes in different work areas.

Materials entering the experimental area must be disinfected first, and enter through the double-door interlocking transfer window; Materials in the laboratory must also pass through the transfer window before they can be transferred to the clean common areas outside the laboratory. The common differential pressure design of PCR laboratory is to form a one-way flow of air pressure between the experimental areas, and the air pressure gradually decreases from the reagent preparation area to the product analysis area. However, if only a pressure difference is formed between the four test areas, only one of the two effects can be achieved: "preventing outside air from entering the test area" or "preventing air from flowing out of the test area".

Shanghai's CEIDI Xidi has done a lot of projects in this regard, you can find them to learn more about it! They are different from ordinary tooling and decoration companies, and they are clean engineering integration service providers with general contracting qualifications.

With many years of experience in the design and construction of the XIGE laboratory, the conversion of the 1500 plant into a PCR sequencing laboratory was a relatively complex project that required an in-depth understanding of the functions and requirements of the laboratory. Here are some design suggestions:

SICOLAB - laboratory engineering and construction service provider.

Needs analysis: First, it is necessary to clarify the needs of PCR sequencing laboratories. This includes requirements for the purpose of the laboratory, the experimental process, the number of personnel, equipment requirements, safety and environmental protection, etc.

Regional division: According to the needs, the factory is divided into different areas, such as sample processing area, PCR detection area, data analysis area, reagent storage area, cleaning area, etc. Each area should be relatively independent and clearly marked and buffered.

Ventilation system design: PCR sequencing experiments have high requirements for air cleanliness, so a reasonable ventilation system needs to be designed. The system should be able to control the direction of airflow and the number of air changes in the laboratory, and be equipped with high-efficiency filters to ensure air quality.

Water supply and drainage system design: The laboratory should be equipped with an independent water supply and drainage system to ensure the adequacy and quality of water sources. Drainage systems should be able to effectively collect and treat wastewater to prevent environmental pollution.

Electrical design: According to the needs of the laboratory, design a reasonable electrical system, including power supply, lighting, sockets, etc. Considering the special requirements of the experimental equipment, the power supply should be equipped with the corresponding voltage regulator and UPS equipment.

Safety facilities: PCR sequencing experiments involve biosafety risks, so it is necessary to set up corresponding safety facilities, such as biological safety cabinets, protective clothing, gloves, etc. At the same time, emergency eyewash and fire extinguishing equipment should be equipped.

Environmental protection facilities: According to the type of waste generated by the laboratory, design corresponding environmental protection treatment facilities, such as wastewater treatment devices, waste gas purification equipment, etc.

Intelligent design: In order to improve the efficiency and safety of the laboratory, intelligent design can be introduced, such as laboratory management system, equipment automation control system, etc.

Personnel training: Laboratory staff should carry out professional training, be familiar with experimental procedures and operation specifications, and master safety and environmental protection knowledge.

In short, the transformation of a factory into a PCR sequencing laboratory requires comprehensive consideration of process, function, safety, environmental protection and other aspects. It is recommended to find a professional design team or company to design and transform to ensure the quality and safety of the laboratory. At the same time, the relevant national norms and standards should be followed in the transformation process to ensure the compliance and reliability of the laboratory.

For this, it is recommended to find a professional company for consultation, after all, PCR laboratory design can not be solved by relying on an article.

PCR laboratory planning and design SICOLAB

1. Reagent preparation and storage area.

It is used for the preparation and storage of reagents (including commercial reagents), and the preparation and dispensing of all reagents.

2. Nucleic acid extraction area.

It is used for sample preparation, nucleic acid extraction, purification and storage, nucleic acid extraction quality inspection, etc. Vessels used for sample preparation should be thoroughly cleaned and autoclaved and used separately. Measures should be taken to eliminate the contamination of nucleic acids in used utensils, otherwise they should not be reused.

Laboratories performing RNA testing should have a dedicated RNA manipulation area in this area.

3. Nucleic acid amplification area.

It is used for the preparation of amplification reaction systems and the addition of templates, nucleic acid amplification.

4. Amplification product analysis area.

It is used for the detection and confirmation of amplified products. If the laboratory only uses a fully automated amplification detector (such as a real-time PCR instrument), the nucleic acid amplification region and the amplification product analysis region can be combined into one region.

The overall design and construction of SICOLAB laboratory: including clean purification engineering, ventilation and exhaust engineering, special water supply and gas supply engineering, electrical engineering, access control and network engineering, intelligent control engineering, laboratory cabinet customization engineering, laboratory decoration engineering, laboratory waste gas treatment engineering.

SICOLAB provides laboratory design and planning solutions, laboratory construction plans, laboratory decoration solutions, laboratory renovation solutions, and laboratory design and decoration solutions.

Gene amplification laboratory design sicolab

One is the dispersed form, and the other is the combined form.

1. The dispersed form refers to the scattered arrangement of the experimental rooms that complete the above experimental process at a distance from each other. For PCR labs in this arrangement, there are no special requirements as the individual experiments do not easily interfere with each other.

2. The combined form refers to the experimental rooms that complete the four PCR experimental processes are adjacent to each other and centrally arranged to form a complete and independent experimental area. Due to the centralized arrangement of each experimental room, it is easy to cause mutual interference, so there are strict requirements for the overall layout and barrier system.

General Air has an overall solution for hospitals that need to establish PCR laboratories, including the construction of PCR laboratories, equipment and reagent selection, and the establishment of testing methods. At the same time, it can provide a full set of PCR experimental instruments and equipment, and is committed to matching customers with better equipment performance, meeting various equipment integration needs, and creating a more efficient and safer experimental environment.

Sicolab is designed with this layout:

1. In principle, the clinical gene amplification testing laboratory is divided into four separate working areas

1. Reagent storage and preparation area.

2. Specimen preparation area.

3. Amplification reaction mixture preparation and amplification area.

4. Amplification product analysis area.

If a fully automated analyzer is used, the zones can be combined appropriately.

2. Each working area must be clearly marked to avoid mixing equipment and items in different working areas.

3. Entering each working area must be carried out in strict accordance with a single direction, that is, reagent storage and preparation area, specimen preparation area, amplification reaction mixture preparation and amplification area, and amplification product analysis area.

The overall design and construction project of SICOLAB laboratory, the construction project of biobank, the construction project of GMP environment, and the environmental protection project.

Sicolab has done a lot of PCR laboratory engineering this year, and there are also mobile PCR nucleic acid laboratories.

1. PCR laboratory design specification SICOLAB

PCR (polymerase chain reaction) laboratory is also known as gene amplification laboratory, "Interim Measures for the Management of Clinical Gene Amplification Testing Laboratories" (Wei Yi Fa [2002] No. 10), "Clinical Gene Amplification Testing Laboratory Work Specifications" (Wei Jian Zi [2002] No. 8) Second, the basic requirements for the design of nucleic acid laboratories are SICOLAB

1. Reagent preparation area: It is the most "clean" area in the PCR laboratory, and it should not have any nucleic acid components, otherwise it will seriously affect the accuracy of the test results. Specimen preparation should be performed in a biosafety cabinet.

Main equipment: refrigerator, cryogenic freezer, mixer, micro-dispenser (mobile ultraviolet lamp.

2. Sample preparation area: nucleic acid extraction, storage, amplification reaction tube production.

Main equipment: refrigerator, ultra-low temperature freezer, high-speed refrigerated centrifuge, mixer, micro-dispenser (mobile ultraviolet lamp, water bath, biological safety cabinet.

3. Amplification area: The configuration of the amplification reaction system and the extraction and addition of nucleic acids should be carried out in the amplification area.

Main equipment: amplifier, centrifuge, microsampler (mobile UV lamp.

4. Amplification product analysis area: the last working area of the product analysis area, which is used for the analysis of amplified fragments.

Main equipment: gel imaging analyzer, electrophoresis instrument, electrophoresis tank, micro-injector (mobile ultraviolet lamp.