Rheumatoid factor determination, relief, rheumatoid factor determination

Dear, I'm here to help you. The initial symptoms of rhoid arthritis are joint pain. Pre-wet-like arthritis presents with joint swelling and tenderness, often in painful joints, and is a sign of synovitis or surrounding soft tissue inflammation to varying degrees depending on the severity of inflammation.

May result from joint effusion or synovial hypertrophy. Osteoproliferative hypertrophy is more common in osteoarthritis. Your condition should be treated as soon as possible, whether it is physical or chemical**, it is best for your physique.

For this kind of condition, you can carry out self-**, a proper massage of the joints, which will help the condition to improve, and **training with the help of the dead, I wish you a speedy **. It is recommended that patients should pay more attention to keeping their bodies warm, exercise more, do not eat more high-fat food, and exercise but should not excessively. Many joints in the body are painful, especially in rainy weather, the knee joints of the hands and feet are particularly painful, and it can be preliminarily determined that it is Feng wet.

During the period when there is no attack of dampness, antibiotics should be supplemented in an appropriate amount to prevent the attack of dampness. In addition, I told a foreign scientist about the latest scientific and technological achievements, and Japan's Shu Tokang is indeed more effective at the moment, and it has been unanimously recognized as the latest achievement of scientists. Importantly, the purchase channel is hard.

You can do a blood test, the test is very simple, and the time is short, within an hour, you can do rheumatism and erythrocyte sedimentation rate.

Rheumatoid factor (RF) is found in the serum of patients with rheumatoid arthritis (RA), is an autoantibody with denatured IgG as the target antigen, mainly found in the serum and synovial fluid of patients with rheumatoid arthritis, it is an anti-denaturing IgG antibody, belonging to the IgM type. Can be combined with IgGFC segments. RF-producing B cell clones are present in RA patients and about 50% of healthy people, and RF can be synthesized in large quantities under the direct action of denatured IgG (or antigen-bound IgG) or Epstein-Barr virus.

In healthy people, there are fewer cell clones that produce RF, and the soluble factors secreted by monocytes can inhibit RF production, so they are generally not easy to detect. RF is mainly IgM autoantibodies, but there are also IgG, IgA, IgD, and IgE. The clinical significance of each type of RF varies.

The determination of IgG, IgA, and IgM RF is usually performed by ELISA indirect method, that is, the microwells of the reaction plate are coated with rabbit IgG by thermoagglutination, and after being added to the sample, anti-human IgG, IgA, and IgM enzyme labeling antibodies are added respectively to make the reaction and then colored with the substrate. To prevent the various Ig class RFs from interfering with each other, enzyme-labeled antibodies use labeled antibody F(Ab)2 fragments. Denatured IgG is coated on polystyrene latex particles, and when this sensitizing latex meets the RF in the serum to be tested, it is called the latex agglutination test.

In the microwells of the polystyrene reaction plate coated with thermoagglutination denaturation, the serum to be tested is added, and if RF is present, it is bound to each other, and then the enzyme-labeled thermoagglutinated IgG is added to react with it, and the color can be developed after the substrate is added. The presence and level of RF can be determined according to the degree of color development, which is inactivated by the 30-minute dual-antigen sandwich ELISA method (inactivates C1Q to prevent false-positive agglutination).

The serum to be tested is diluted 1:20 with glycine-buffered saline (add serum to 1 ml of normal saline.

2. Take 1 drop of this diluted serum (approximately, add it to the square of the black checkered slide, add 1 drop of latex RF reagent, immediately shake the reaction plate for 3 minutes, mix it thoroughly, and observe it under direct light. A positive and negative control was provided for each test. The normal latex agglutination test is mostly negative, and those with obvious agglutination within 3 minutes are positive.

Specimens with positive reaction should have their serum diluted to determine their titer. The ELISA method can be judged as positive or negative according to the degree of color rendering and the reference between the positive and negative controls.

Rheumatoid factor (RF) can be divided into five types: IgM, IgA, IgG, IgD, and IgE (Note: It is described as type 4 in clinical internal medicine, and there is no IgD type; However, it is described as type 5 in laboratory diagnostics), which is a class of autoantibodies against the epitope on the IgG FC fragment in the serum of rheumatoid arthritis, and patients with positive rheumatoid factor are more likely to have extra-articular manifestations such as subcutaneous nodules and vasculitis. IgM RF prevalence ranges from 60% to 78%.

Hello! Rheumatoid arthritis is often differentiated from the following:

1.Osteoarthritis.

2.Reactive arthritis.

3.Ankylosing spondylitis.

4.Psoriatic arthritis.

5.Systemic lupus erythematosus.

Anti-cyclic citrullinated peptide (CCP) antibody is a highly specific new indicator of RA, with a specificity of 96 for RA and a sensitivity of 80, which can be positive at a very early stage of RA, providing an important basis for early diagnosis of RA, and the positive rate in other rheumatic diseases is very low.

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90%, but its specificity is not high. In addition to rheumatoid arthritis, rheumatoid factor positivity may be caused by:

1.Other rheumatic diseases: lupus erythematosus, Sjögren's syndrome, systemic sclerosis, 2Normal healthy people.

3.Acute viral infections: acute mononucleosis, hepatitis, post-vaccination.

4.Parasitic infections: malaria, schistosomiasis.

5.Chronic infections: tuberculosis, leprosy, syphilis, subacute bacterial endocarditis.

6.After chemotherapy or radiotherapy for malignant tumors.

7.Other high globulin states: purpura, sarcoidosis, chronic liver disease, cryoglobulemia Therefore, a negative rheumatoid factor does not exclude rheumatoid arthritis, and a positive test does not confirm the diagnosis of rheumatoid arthritis, but a persistently high titer positive rheumatoid factor in rheumatoid arthritis predicts a poor prognosis, with a higher incidence of bone destruction and extra-articular manifestations.

I'll talk about that. How can we take care of ourselves in life to avoid rheumatism? Winter rheumatic porridge can be described as a miracle soldier, in the coldest season, boil some winter rheumatic porridge every dinner, you can add some reinforcements to your righteousness, reduce the torture of rheumatism.

In fact, the method is also very simple, 10 grams of cinnamon sticks, 100 grams of rice, 2 white green onions, and 3 slices of ginger. After washing the cinnamon sticks, put them in a pot, add an appropriate amount of water and soak for about 10 minutes, and then fry them in water to extract the juice. Put the rice into the fried cinnamon water and boil, when the porridge is almost cooked, put the washed green onion and ginger slices into the pot, and boil it twice.

Consume 3 to 5 times in a row. It should be noted that the porridge must be eaten while it is hot, but don't wait until the porridge is almost cold before eating, so that the effect will not be obvious. In addition, Japan's Ryukang is very applicable to your situation and has been unanimously recognized, and I personally sincerely recommend it to you.

The egg hurts, it's so expensive.

Hello, rheumatoid factor is an antibody produced in the body due to infectious agents (bacteria, viruses, etc.) that use denatured IgG (an antibody) as an antigen.

elevated: seen in rheumatoid arthritis, systemic lupus erythematosus, and other connective tissue disorders (eg, Sjögren's syndrome, scleroderma, polymyositis); The number of patients with chronic hepatitis and the elderly is also increasing.