LPS treatment of bacterial gene expression at different times as to why the highest value is reached

Peroxides such as hydrogen peroxide, ammonium persulfate, and potassium persulfate were selected to form different concentration gradients, and the tested substances were added to form different selective pressure gradients, and the co-culture was held for a certain period of time. It is sufficient to determine the peroxidase activity or peroxide content at different concentration gradients.

Because the amount of the band you want may not be the expression of the gene itself, but the amount of template DNA, so in order to rule out this possibility and error, you need to use the internal reference gene to represent the amount of all DNA. Therefore, when calculating, the relative expression of the target gene can be obtained by dividing the reference gene by the reference gene.

It should be different. Because if its genes are different, then the products it expresses are also different.

Is meca a gene? If so, then the positions of genes taken in the hands of the ascension in different literature may be different, and the positions of amplification may also be different. Are you doing this PCR to see if this gene is present, or is it something not to pretend to be?

If you just want to see if there is any noise, then just use a pair of primers inside.

Just do a PCR.

According to the law of heat death of microorganisms Logarithmic Residue Law:

At a certain temperature, the change in the number of dead cells after microorganisms are heated is the same as the change in the concentration of chemical reactions, and there is a certain regularity.

The rate of microbial thermal death is related to the number of microbial surviving cells, that is, the rate of microbial thermal death is directly proportional to the number of microbial viable cells remaining at any moment

nt=n0*e (-kt) logarithmic residue law.

Residual curves of E. coli at different temperatures, the higher the temperature, the greater the k value, and the easier it is for microorganisms to die.

The residual curve of some microorganisms is not a straight line, because the microorganisms are viable vegetative cells and heat-tolerant spores, the higher the temperature and the greater the k, the more likely the microorganisms are to die.

The k-value of the spores is much smaller than that of their vegetative cells.

The same microorganism has different k values at different sterilization temperatures: the lower the sterilization temperature, the smaller the k; The higher the temperature, the greater the k-value, and the faster the microorganisms die. The higher the sterilization temperature, the greater the K value, the shortening of the sterilization time, and the sterilization of the medium: the oils, sugars and proteins in the medium will increase the heat resistance of microorganisms for a short period of time; High concentrations of salts and pigments will reduce their heat resistance.

Sterilization conditions are intensified, the composition in the medium changes, sugar caramelization, protein denaturation, vitamin inactivation, aldose and amino compound reactions, unsaturated aldehyde polymerization, hydrolysis of some compounds.

The medium is heated at high temperature for a short time.

In the biological field, it is expressed as the amount of environmental capacity. Environmental capacity (k-value) It is not fixed (1) The k-value of the same organism is not fixed and will be affected by the environment, and the k-value will fluctuate around the average value if the environment is not damaged. (2) when the environment is damaged, the k-value decreases; When the living environment of organisms improves, the k-value increases.