How to use the 721 spectrophotometer

Learn how to do the standard curve first, and you'll be done. If you want to use a blank to zero, that is, put down the blank, point 100%, and then you can take other samples to compare colors.

It is recommended that you familiarize yourself with the manual first to understand the significance of each button and panel.

1. Turn on the machine and preheat it for more than half an hour (a common problem for domestic products) 2. Select the wavelength you need;

3. Adjust the zero point, put in the black body, and press 0%.

4. Adjust 100, put in a blank cuvette or cuvette filled with distilled water, press 100% 5, according to your test, make a standard curve, and determine the unknown concentration of the substance. I wish you a speedy solution to the problem and proficiency.

Xinxiang Medical College Sanquan College - 722 spectrophotometer use.

The 722 Spectrophotometer instrument is suitable for the visible spectrum.

The content of substances in the region is quantitatively analyzed, which can be widely used in basic laboratories of factories, schools, agriculture, food, biochemistry, environmental protection, medical and health units. The steps are as follows:

1. Adjust the sensitivity knob to "1" gear (minimum signal amplification).

2. Turn on the power supply, the indicator light is on, the selection switch is set to "T", and the wavelength is adjusted to the wavelength for testing. The instrument is warmed up for 20 min.

3. Open the sample chamber (the light door will close automatically) and adjust the light transmittance.

Zero knob so that the numbers are displayed as. (adjust the 100% t knob), close the sample chamber lid, and place the cuvette rack in distilled water.

Correct the position so that the photocell receives light, and adjust the light transmittance 100% knob to make the digital display.

If it is not displayed, the multiple of microcurrent amplification can be increased appropriately. (Increase the number of gears of sensitivity, and at the same time repeat (3) adjust the "0" position of the transmittance of the instrument), but try to make the magnification use at a low level. In this way, the instrument will have higher stability.

4. After preheating, adjust the position of "0" and "100%" of light transmittance several times in a row, and the instrument can be measured after stabilization.

Notes:

This instrument is suitable for working in a stable laboratory environment, and the installation conditions are:

Ambient temperature: 5° 35°

Ambient humidity: 85%.

Power supply voltage: 220V 10% 50Hz

The rear side of the main unit is more than 20 cm from the wall.

Avoid direct sunlight, avoid vibrations, avoid dust, avoid corrosive substances.

Dear, I am glad to answer for you: How to use 721g visible spectrophotometer A1Turn on the power supply, turn on the instrument switch, lift the lid of the dark box in the sample chamber, and warm up for 10 minutes.

2.Set the sensitivity switch to "1" (if the zero adjuster cannot be set to "0", you need to use a higher setting.) ） 3.

Turn the wavelength selector according to the desired wavelength. 4.Pour the blank solution and the measuring solution into the cuvette 3 4 respectively, wipe the outer wall with a mirror paper, put it into the sample chamber, and align the blank tube with the optical path.

5.Adjust the zero regulator with the camera obscura lid open so that the readingdial pointer points to t=0. 6.

Cover the lid of the camera obscura, adjust the "100" to adjust the slapper, so that the t = 100 of the blank tube, and gradually pull out the sample slide rod after the pointer is stable, read out the optical density value of the measuring tube respectively, and record. 7.After the color comparison is completed, turn off the power, take out the cuvette and wash it, and wipe the sample chamber with a soft cloth or soft paper.

1. Check whether the starting position of each adjustment button of the instrument is correct, turn on the power switch, open the dark box cover of the sample chamber, make the meter pointer in the "0" position, preheat for 20min, and then select the monochromatic light wavelength and the corresponding amplification sensitivity file, and adjust the meter to t=0% with the "0" potentiometer.

2. Close the cover of the sample chamber to make the photocell receive light, push the handle of the sample rack, make the reference solution pool (the solution is loaded into 4 5 heights, set the first grid) on the optical path, and adjust the 100% transmittance regulator to make the meter pointer t=100%.

The sensitivity of each level of the amplifier is: "L" 1 times; "2" 10 times; "3" 20 times Huiyou, the sensitivity increases sequentially. Because the wavelength of monochromatic light is different, the light energy is different, so different sensitivity levels need to be selected.

The selection principle is to use a lower sensitivity setting to improve the stability of the instrument when the reference solution can be adjusted to t= 100%. After changing the sensitivity level, "0" and "100" should be re-adjusted.

3. Repeat the operation of opening the sample chamber cover, adjusting 0, closing the sample chamber cover, and adjusting the transmittance to 100% until the instrument is stable.

4. Close the lid of the sample chamber and push the handle of the sample holder to place the sample solution pool on the optical path and read out the absorbance value. The sample chamber lid should be opened immediately after the reading.

5. After the measurement, take out the cuvette, wash it and put it on the filter paper to dry. Place each knob in its original position, turn off the power supply, and unplug the power supply.