How should whole genome sequencing data be analyzed after acquisition?

You need to be clear about what output you're going to end up getting.

If you want to use your existing data to build models for diagnostics or **, go out and turn left on biogenesis and machine learning.

If you want to annotate new genes with your existing data, go right to natural language processing and genome assembly.

If you want to use your existing data to find disease-related gene pathways in the network, go straight to differential expression analysis. However, first of all, you have to have a server that can run, secondly, you have to have the attitude of reading the literature by yourself and not reaching out to the party, and finally, if you are new to NGS, it is recommended to start with a certain type of data (such as RNA-seq), and do not touch other data before eating a class, otherwise you will really cry when you make all the type I errors. <>

It is important to note the following points: in the assembly process, the assembly software assembles the sequencing data as if it came from the same genome; If there is exogenous DNA pollution, there will be different degrees of similarity sequences and non-similarity sequences in different ** DNA, and these complex relationships will interfere with the assembly software, and the software can only cut off the suspicious part into different fragmentation sequences in order to ensure the accuracy of the assembly, resulting in the final assembly result can only obtain fragmented sequences, and lose the effect that the assembly itself wants to achieve; If a sufficiently closely related reference genome can be found for contamination isolation, the above results can also be improved to some extent. However, because the exogenous DNA itself may carry certain similar sequences, and there will be potential differences between the target genome and the reference genome, the segregation results will produce certain false positives and false negatives. In summary, even if the assembly is performed after contamination isolation, it is not possible to meet the assembly standard of pure DNA.

1. Enrichment analysis (KO) sample requirements sample collection: the consistency of sample collection conditions is the most important link, sampling in strict accordance with the sampling standard, and sealing the sample for cryoppreservation immediately after sampling.

2. There is still some distance, so you can get your complete Qingzhuxiao primer sequence. Since there will always be some bases at the beginning of the sequencing that cannot be read accurately, it is best to clone and sequence if you want to get the full sequence of your PCR product.

3. Answer: To engage in gene sequencing work, you should study (biology). Genomics (biology) is the study of biological genomes and how to use genes.

4. First of all, the gene classification is carried out, such as the proportion of coding genes in the manuscript and the proportion of non-coding genes; What is the proportion of transcription factors, what is the proportion of protein kinase genes, and so on. The genome of the species is then compared to other sequenced genomes, including size, homology, etc.

5. The total number of bases, totallymappedreads, and uniquelymappedreads are counted from the data, and the sequencing is analyzed in depth.

Whole genome sequencing is the sequencing of the genome of an individual for a species of unknown genome sequence. The significance of the whole genome reveals the mystery of human life, aging, illness and death, so that human beings can fundamentally understand the causes of diseases, achieve correct diseases and prevent diseases as soon as possible. Each person inherits the DNA genetic information of their parents from the time of the fertilized egg, and carries it for a lifetime, which is not easy to change.

Whole genome sequencing uses a new generation of high-throughput DNA sequencers to perform 10 to 20 times the coverage of the whole genome of individuals, and then compare it with the exact map of the human genome to obtain a complete personal whole genome sequence and decipher all the genetic information of individuals. Whole genome sequencing covers a wide range of genomes and can detect all genetic information in an individual's genome with high accuracy.