What does TLC mean? What does TLC mean:

Thin layer chromatography (thin layer chromatography) is an important experimental technique for the rapid separation and qualitative analysis of a small number of substances, which belongs to solid-liquid adsorption chromatography, which has the advantages of column chromatography and paper chromatography, on the one hand, it is suitable for the separation of a small number of samples (a few micrograms, or even micrograms); On the other hand, when making thin layer plates, the adsorption layer is thickened and enlarged.

Therefore, it can also be used to refine samples, and this method is particularly suitable for volatility.

Substances that are small or high in temperature and are subject to change and cannot be analyzed by gas chromatography. It is an analytical method in which adsorbents, carriers or other active substances are evenly coated on flat plates (such as glass plates, plastic sheets, metal sheets, etc.) to form a thin layer (commonly known as about millimeters), and then chromatography separation is carried out on this thin layer.

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TLC isThin layer chromatographyThe full name is thin-layer chromatography.

Thin layer chromatography (TLC) usually refers to a type of liquid chromatography that uses an adsorbent as the stationary phase. That is, the stationary phase is evenly spread into a thin layer on a smooth surface such as glass, metal or plastic, the sample is dotted at one end of the thin layer, and the mobile phase acts by capillary.

The substance flows through the stationary phase so that it is separated.

Precautions for TLC operation:1. After the thin layer is passed through the spatula or trap, the adsorbent of the spot is captured and prematurely collected, and then eluted with an appropriate organic solvent, and then the visible and ultraviolet spectrophotometry is used.

The content was determined by fluorescence spectrophotometry.

2. The sample spots can not be sprayed with chromogenic agent, so as not to affect the determination method of the spot position without measurement.

3. Samples in ultraviolet light.

Under the emission of fluorescence, it can be used in ultraviolet lamps.

Observe the spot below and use a needle to scratch off the spot location.

4. Use Shidian steam to develop color, and treat brown spots in the component (Shidian will volatilize after adsorption).

5. Use the standard to control, cover the sample when the standard point is measured on the edge and determine the location of the spot.

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