What kind of equipment is the fluorescent cell dynamics analysis system?

This is a brand-new device called TAXICAN-FL produced by ECI in Japan, which is a perfect combination of new optical dynamic imaging and live cell processing technology.

The device can:1Different chemotant concentration gradients can be established repeatedly2

Digitally recorded slow-paced and fast-play technology to retain experimental moving images 3Fluorescence imaging captures cellular events in real time 3Automatically focus and track the dynamic evolution of a single living cell 4

The high-throughput test carrier can complete 12 experiments at the same time 5No darkroom environment is required.

The important features of this technology are:1The core component is a silicon-based chip with a horizontal channel embedded on it that forms a chemical chemokine concentration gradient 2

The depth accuracy of the horizontal channel is smaller than that of the suspension cell, and it can be accurate to the level of malaise, in which the morphological changes of cells and the proliferation and migration process can be observed3The imaging component is a cold-light CCD camera positioned below the observation plane and equipped with a high-performance lens and a coaxial anti-illumination device 4Based on the above revolutionary technology, experiments require only 100 or fewer cell samples5

Customize the parameters of the experimental conditions according to the specific requirements of the experiment.

There are 4 main applications:1Cell chemotaxis assay 2Mast cell degranulation 3Simultaneous imaging of chemotaxis and calcium influx 4Encapsulation and reactive oxygen species metabolism.

I haven't really used this before. Judging by the name, it is possible that it is a system for observing the physiology of cells by embedding fluorescent proteins. Ability to monitor in real time. It is generally used to analyze the influence of certain factors on cell viability.

Flow cytometry is used to measure relative fluorescence intensity. So first, there must be a control.

There are several commonly used methods to analyze:

First, set the threshold with the control, and then analyze the change of the percentage of each group that exceeds the fluorescence intensity threshold, if there is no obvious positive and negative threshold, you can compare the overall fluorescence intensity mean, median, etc., which index to use, depending on the purpose of the experiment.

Fluorescent dyes, due to their high sensitivity and easy operation, have gradually replaced radioisotopes as detection markers, which are widely used in fluorescent immunoassay, fluorescent probes, cell staining, etc. Including specific DNA staining for chromosome analysis, cell cycle, apoptosis and other related studies. There are also many nucleic acid dyes that are very useful counterstains in multicolor staining systems, which can be used as background controls to label the nucleus and make the spatial relationships of intracellular structures clear at a glance.

Nucleic acid fluorescent dyes can quantitatively measure the fluorescence intensity emitted by cells after staining the nucleus, and the content of DNA and RNA in the nucleus can be determined, and the cell cycle and cell proliferation can be analyzed. There are a variety of fluorescent dyes that can stain DNA or RNA in cells, commonly used DNA dyes include propidium iodide (PI), DAPI, Hoechst 33342, etc., and RNA dyes include thiazole orange, acridine orange, etc.

protein localization studies;

interaction studies;

Cell signaling studies.

Recommended to know: Immunofluorescence is a method that combines immunological methods (antigen-antibody specific binding) with fluorescent labeling techniques to study the distribution of specific protein antigens within cells. Since the fluorescence emitted by fluorescein can be detected under a fluorescence microscope, antigens can be cellularly localized.

The abscissa represents the relative fluorescence intensity, and the ordinate represents the cell count.

The meaning of the graph is how many cells are in each fluorescence intensity.

Estimate that you used an oxidation-sensitive fluorescent pointer and the fluorescence increased after the ROS reaction. Therefore, you should first set a threshold with the control group to see if the fluorescence intensity of the experimental group is higher or lower than the threshold.

Immunohistochemistry and immunofluorescence are both tests for protein localization (i.e., to determine whether the protein is expressed in the plasma of the nucleus).

Immunohistochemistry, also known as immunocytochemistry, refers to a new technology for the qualitative, localized and quantitative determination of specific antibodies labeled with chromogenic agents in tissue cells through antigen-antibody reaction and histochemical color reaction in situ. It skillfully combines the specificity of the immune response with the visibility of histochemistry, and with the help of microscopy and magnification, it can detect various antigenic substances at the cellular and subcellular levels, and can display the corresponding genes and gene expression products in situ. Immunohistochemistry techniques are now available:

Immunofluorescence tissue (cell) chemistry technology, immunoenzyme tissue (cell) chemistry technology, affinity tissue chemistry technology, immunogold and silver and iron labeling immunohistochemistry technology, etc. Immunofluorescence tissue (cell) chemistry technology is to use fluorescein-labeled known antibodies (or antigens) as probes to detect the target antigen (or antibody) in the tissues and cell specimens to be tested, and the antigen-antibody complex formed has fluorescein, under the fluorescence microscope, due to the ultraviolet light irradiation of the high-pressure mercury light source, fluorescein emits bright fluorescence, so that the location and properties of the antigen (or antibody) can be distinguished, and the content of the antigen can be calculated by fluorescence quantification technology. In order to achieve the purpose of localization, qualitative and quantitative determination of antigenic substances.