How many times does it take to observe algae?

Observe with a microscope of 600x or higher.

The main method. 1. Bacteria in tartar.

Use a sterilized toothpick to pick some debris between your teeth, put it in the water droplets on a clean glass slide, mix thoroughly, and make a smear. After the smear is dry, light in alcohol.

The flame slowly swept back and forth 3 or 4 times, and some of the gum on the outside of the bacteria adhered to the glass slide, so that the bacteria were fixed and slightly cooled, and a drop of methylene blue was added.

solution, stain for 1 2 min later, rinse with water, and then cover with a coverslip. Under the microscope, first observe with a low magnification, and then change to a high magnification, bacilli, vibrio, cocci, etc. can be observed. If you use an oil lens to observe, the effect is clearer and more obvious.

2. Bacteria in the manure tank.

In the manure tank, use a straw to suck a drop of the upper layer of the fecal mixture, drop it on a clean glass slide, and cover with a coverslip. Observe under a microscope, first with a low magnification and then with a high magnification. Live spirochetes may be seen.

and bacilli. Note that the light should be dim when observing, and the effect is good.

3. Bacteria in sour rai and yogurt.

Take the white film or yogurt clarifier on the upper part of the sauerkraut liquid, make a temporary tablet, and observe it under a high-magnification microscope, and the lactobacilli can be seen.

4. Observation of rhizobia.

Take legumes.

Soybeans, broad beans, peanuts] are cut with a blade, a small piece of content is picked with a needle, placed in a water droplet on a glass slide, made into a smear, dried and fixed, baked and fixed with an alcohol lamp flame, and then dropped a drop of methylene blue solution, stained for 2 3 minutes, rinsed with water, covered with a coverslip, and after blotting, observed with a 500x high magnification, brevis rhizobia can be seen.

5. Bacillus subtilis.

cultivation and observation.

Cut 25g of fresh hay into small pieces and place in a beaker with 200ml of water.

Medium, heat and boil for 15 minutes, when the solution is dark brown, pour into the flask, and place in a dark, warm environment of 20 30. After 2 to 3 days, the liquid becomes cloudy and a thin film forms on the surface. The liquid floating in the upper layer is sucked up with a straw to make a temporary smear, which is viewed under a high-magnification microscope to see the lines of Bacillus subtilis.

If the culture medium is placed at a low temperature for 1 2 days, and then taken out to make a temporary smear, the spores of Bacillus subtilis can still be seen by microscopic observation at 600 times or higher.

How to observe algae with a microscope is to be made into a mount to observe.

First of all, materials: including microscopes, straws, glass slides, coverslips, tweezers, absorbent paper, iodine solution, etc.

Steps: 1. Make the mounting, 2. Dye.

3. Observation.

The difference is that light microscopy can only see certain cellular structures such as cell walls, chloroplasts, stained chromosomes, mitochondria, nucleus, etc., while electron microscopy can see the internal structure of organelles and smaller organelles like ribosomes. The microstructure of the cell is seen with a light microscope, and the submicrostructure can be seen with an electron microscope.

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1000 times, to be able to dye, with cedar oil as an oil mirror, otherwise you can't see clearly. Look at mold 40 times, look at animal plant cells.

Just 400 times.

Common bacteria such as Escherichia coli and Bacillus are visible under an ordinary light microscope, but through a 1000x oil lens, and they need to be stained, generally with crystal violet.

or Sudan dyes single staining is enough to observe bacterial morphology, Gram staining.

The operation is a bit complicated and requires skill and practice.

Special requirements, like the movement of bacteria, require darkfield microscopy observation.

You can see it.

Chlorella can be seen very clearly under the 40x light microscope, but I don't know how clearly you want to see it, if you want to see the internal structure, it is best to use an electron microscope.

Extended reading: Chlorella is a spherical single-celled freshwater algae with a diameter of 3 8 microns and is the earth.