What is the purpose of the multifunctional microplate reader, and what items can be detected by the

The principle of microplate reader is actually a disguised photophotoelectric colorimeter or spectrophotometer, and its basic working principle is basically the same as the main structure and photoelectric colorimeter. The light wave emitted by the light source lamp is turned into a beam of monochromatic light through a filter or monochromator, which enters the specimen to be measured in the plastic microporous pole. Part of this monochromatic light is absorbed by the specimen, and the other part is irradiated to the photoelectric detector through the specimen, and the photoelectric detector converts the different and different intensity of the optical signal of the specimen to be measured into the corresponding electrical signal, and the electrical signal is sent to the microprocessor for data processing and calculation after signal processing such as pre-amplification, logarithmic amplification, analog-to-digital conversion, etc., and the result is displayed by the display and the printer later.

The microprocessor also moves the microplate through a control circuit that controls the movement of the mechanical drive mechanism in the X and Y directions, so as to realize automatic sample injection detection

Microplate readers are versatile.

It is an indispensable medical device for biological testing, which uses enzyme-linked immunoassay to conduct qualitative or quantitative analysis according to the presence and absence of pigments and the shade of color according to the principle of enzyme labeling. This is a very dynamic immunology technique. It can be used for monoclonal antibody screening, coagulation, antibiotic sensitivity testing, and other analytical work that requires colorimetry.

The instrument is suitable for clinical laboratory, microbiology, epidemiology, immunology, endocrinology, and agriculture and forestry science. It is widely used in hospitals, blood banks, epidemic prevention stations, biological products and other departments. Famous instruments.

American wisdom microplate reader.

Microplate reader can detect items: hospital clinical diagnosis, immunopathological detection, microbial antibody detection, parasite diagnosis, blood disease diagnosis, plant disease and pest research, veterinary drug residues, rapid diagnosis of animal diseases, endocrine disorder determination.

The microplate reader is actually a spectrophotometer, that is, the throughput is large, and it is convenient to do a large number of them. You can use it as long as you measure the absorbance value of a certain band (nm). When you know how it works, you know it's not about you or the project.

Microplate reader, plate washer and computer components constitute the enzyme immunization test workstation, in which the microplate reader can be divided into four types of microplate reader medical devices according to the function, namely light absorption microplate reader, fluorescence microplate reader, chemiluminescence microplate reader and multi-function microplate reader.

Species, light absorption microplate reader.

Light absorption microplate reader is used to detect the absorbance of visible light and ultraviolet light, after the light of a specific wavelength passes through the sample in the microplate, the light energy is absorbed, and the absorbed light energy is in a certain proportion with the concentration of the sample, which can be used for qualitative and quantitative detection, the detection technology of light absorption is mature, the cost is low, the operation is simple, but the dynamic range is narrow, the sensitivity is relatively low, and the specificity is not strong. Generally, tungsten lamp and deuterium lamp are used as light sources for visible light and ultraviolet light, while the ultraviolet visible microplate reader can switch between the two light sources to meet the needs of different measurement wavelengths.

The second is a fluorescence microplate reader.

Fluorescence microplate reader is used for the detection of fluorescence, through the excitation grating spectroscopy of a specific wavelength of light irradiated to the fluorescent substance calibrated sample, will emit a longer wavelength of emission light, through the emission grating to reach the detector. The intensity of the fluorescence is proportional to the concentration of the sample. Fluorescence detection has high sensitivity, real-time detection, easy to use, and various detection modes, but it is easy to be disturbed by external interference, and the excitation light and the emitted light are easy to affect each other and interfere with the detection.

The third is the chemiluminescence microplate reader.

Chemiluminescence microplate reader is a self-luminescence from a biochemical reaction, which can be divided into two types: glow type and flash type. The glow type glows long-lasting, stable, and can last for a period of time; The flash type has a short luminescence time, rapid change, and low stability, so it can only be carried out by applying an automatic sampler. The number of photons emitted in chemiluminescence is proportional to the sample size, and the chemiluminescence microplate reader has very high sensitivity and a wide kinetic range.

Fourth, multi-function microplate reader.

The multi-function microplate reader is a collection of the above three microplate readers, which integrates two or three kinds of microplate readers in one, which can detect light absorption, fluorescence and chemiluminescence at the same time, with powerful functions and a wide range of use, which can be used as a research platform to avoid repeated purchases, and is an ideal choice for laboratories. In addition, according to the number of channels, the microplate reader can be divided into two types: single channel and multi-channel, and there are two types of single channel: automatic and manual.

Microplate reader: enzyme-labeled instrument: enzyme labelling: enzyme linked immunosorbent assay

Enzyme labeled compound: enzyme-labeled immunoassay: enzyme-labeled immunoassay:

Nonius Instrument: Coordinatograph

Coordinate Machine:

Microplate reader. It is mainly used to detect absorbance in enzyme-linked immunosorbent detection reactions.

value, whose use is mainly used in the determination of ELISA reagents, is widely used in various laboratories, including clinical laboratories.

The principle of microplate reader determination is to detect the absorbance value of the analyte at a specific wavelength.

Detection unit: The energy difference before and after the light passes through the detected object is the energy absorbed by the detected object, and the concentration of the same detected object has a quantitative relationship with the absorbed energy at a specific wavelength.

The OD value of the detection unit.

od is the abbreviation of optical delnsity, which indicates the optical density absorbed by the detected object, od 1og (1 trans), where trans is the light transmittance value of the detected object. According to the Bouger-Ambert-Beer rule, the OD value is related to the light intensity.

into the following relationship:

e od=log 0 where e is the absorbed optical density, 0 is the light intensity before the test object, and is the light intensity coming out of the test object.

The OD value is calculated by the following formula:

e＝od=c×d×e

c is the concentration of the analyte.

d is the thickness of the test object.

e is the molar factor.

Each substance is determined at a specific wavelength at which it absorbs the most light energy. If a different wavelength band is chosen, the detection results will be inaccurate. Therefore, when measuring the analyte, we select a specific wavelength for the detection, which is called the measurement wave.

Long. However, there is a certain non-specificity of each substance for light energy.

Absorption, in order to eliminate this non-specific macro noisy absorption, we select a reference wavelength to eliminate this inaccuracy. At the reference wavelength, the absorption of light from the detected object is minimal. The difference between the absorbance values of the detection wavelength and the reference wavelength can eliminate non-specific absorption.

Generally, microplate readers are mainly used in the rapid detection of food safety drug residues, such as Clenbuterol with Beijing Wanger Biotechnology.

Chloramphenicol, furazolidone.

Nitrofuranone, nitrofurantoin, sulfonamides, fluoroquinolones, streptomycin, penicillin.

When using ELISA kits for the rapid detection of residues, microplate readers are generally used for quantitative detection. If you need to build a laboratory, Wanger can provide a construction plan and related detailed suggestions for the testing laboratory. Contact:

Microplate reader:enzyme-labeled instrument:enzyme labellingenzyme labelling:

enzyme linked immunosorbent assay

enzyme labeled compound: enzyme-labeled immunoassay: enzyme-labeled immunoassay:

Nonius Instrument: Coordinatograph

Coordinates: 1 coordinatograph2. coelosphere

Quite simply, Elisa. In this way, everyone who speaks English will understand. Leave well enough alone.

1.The working environment microplate reader is a precision optical instrument, so a good working environment can not only ensure its accuracy and stability, but also extend its service life.

a.The instrument should be placed in a location free of strong magnetic fields and interfering voltages.

b.The instrument should be placed in an environment with a noise level of less than 40 decibels.

c.To delay the aging of optical components, direct sunlight should be avoided.

d.The operating ambient temperature should be between 15 and 40, and the ambient humidity should be between 15% and 85%.

e.The voltage should remain stable during operation.

f.The operating environment is clean and clean, avoiding water vapor and smoke.

g.Keep a dry, clean, level work surface with plenty of space to work with.

2.Precautions for the use of microplate readers.

a.Use a pipette to fill the liquid and pipette tips should not be mixed.

b.Wash the plate cleanly to avoid cross-contamination.

c.Strictly follow the instructions of the kit, and the reaction time is accurate.

d.Do not spill samples or reagents on the surface or inside of the instrument, and pay attention to cleaning after the operation is completed.

e.Do not turn off the power during the measurement.

f.For the deviation of the measurement results caused by the kit problem, the parameters should be modified in time according to the actual situation to achieve the best results.

g.Cover the dust cover after use.

In case of technical failure, contact the manufacturer in time, and do not disassemble the microplate reader without authorization.

I also want to know when it will be available?

I checked a lot of information and said that it was fast, but I don't know how fast it will be!