On the topic of biological cells, what are the key questions in cell biology that you are concerned

Choose A, pre-mitotic DNA replication, the mass should be 2 times that of normal somatic cells, sperm cells are 4, and somatic cells should be the same as fertilized eggs

Most of the DNA is in the nucleus, but a small amount of DNA is found in other organs (e.g., DNA in the cytoplasm).

This is the reason why identical twins are not genetically identical.

The key questions of cell biology that you are concerned about are fundamental to the life sciences.

Cell biology is the science that studies and reveals the basic life activities of cells, and it studies the structure and function of cells at the microscopic, submicroscopic and molecular levels. Cell proliferation, differentiation, metabolism, movement, aging, death, as well as cell signal transduction, cell gene expression and regulation, cell origin and evolution and other major life processes.

Cell biology is a discipline that studies the structure and function of cells and organelles, the life history of cells, and various life activities from a dynamic perspective, from the three levels of cells: the overall water level, the submicroscopic level, and the molecular level. Cell biology is one of the cutting-edge sub-disciplines of modern life science.

It is mainly from the different structural levels of cells to study the basic laws of cell life activities. From the perspective of life structure, cell biology is located between molecular biology and developmental biology, and they are interconnected and interpenetrated. The core issue of the science that uses the technological achievements of modern physics and chemistry and the methods and concepts of molecular biology to study life activities at the cellular level is the problem of heredity and development.

The combination of cell biology and molecular biology is getting closer and closer, and the study of cell bisecting structure and its role in life activities has become the main task, and gene regulation, signal transduction, tumor biology, cell differentiation and apoptosis are the hot topics of contemporary research.

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Lung. 2.Relative humidity.

4.Thoracic cavity of the heart.

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A (I just checked, absolutely yes).

5a (Bacteria are prokaryotes, it has a cell wall and its components are peptidoglycan. The cell wall of the plant is composed of cellulose and pectin, the fungus is mostly chitin, and the yeast is cellulose and mannan) and so on!

Yeasts are facultative anaerobic organisms. When hypoxic is low, anaerobic respiration is carried out to produce alcohol, which can be used for sake brewing. Aerobic respiration is carried out during high oxygen to produce carbon dioxide, which can be used for pastry fermentation.

Paramecium are aerobic respiration organisms that cannot survive when hypoxic and start from 0.

Lactic acid bacteria are anaerobic organisms, and their respiration intensity is highest when they are hypoxic, and their respiration is inhibited by the increase in oxygen concentration, so the intensity decreases.

Stain first, then look under a fluorescence microscope (e.g., confocal).

DAPI is 4', 6-diamidino-2-phenylindole (4', 6-diamidino-2-phenylindole), is a fluorescent dye that binds strongly to DNA and is commonly observed with fluorescence microscopy[1]. Because DAPI can permeate intact cell membranes, it can be used to stain both live and fixed cells.

Under fluorescence microscopy, DAPI dyes are excited by ultraviolet wavelengths. When DAPI is combined with double-stranded DNA, the maximum absorption wavelength is 358 nm and the maximum emission wavelength is 461 nm, and the wavelength range of divergent light covers blue to turquoise. DAPI can also bind to RNA, but the fluorescence intensity is not as strong as that of binding to DNA, and the wavelength range of its divergent light is about 400nm.

The divergent light of DAPI is blue, and the divergence wavelengths of DAPI and Green Fluorescent Protein (GFP) or Texas Red stain (TEXAS RED) overlap only slightly, allowing researchers to perform multiplex fluorescence staining on a single sample.

DAPI can quickly enter living cells and bind to DNA, so DAPI is also regarded as a toxic substance and carcinogen for living organisms. Attention should be paid to the handling procedures for operation and disposal during use.