How to properly use an autoclave to sterilize the medium

First, check whether the amount of distilled water in the pot is sufficient, and second, check whether the valves and switches are working properly. Third, wrap the items to be sterilized with a layer of kraft paper or two layers of newspaper, and be sure to wrap it completely! Put it in the pot, and there must be a gap between the objects.

Fourth, cover the pot, tighten the nut symmetrically, open the air valve, and close the safety valve. Fifth, turn on the power supply to observe whether there is steam outflow out of the gas release valve, close the air release valve after confirmation, and time the pressure gauge when it reaches the temperature of about 121), 15-30 minutes, turn off the switch. Sixth, after the pressure gauge pointer is reset to zero, open the air valve, open the lid in a symmetrical way, and take out the items.

Notes:1The water in the pot must be distilled or purified.

2.The lid nut must be tightened symmetrically. 3.

Be sure to vent the valve with plenty of steam out when it is closed. 4.When the sterilization time is over, it is necessary to wait until the pressure gauge is turned on at zero.

5.When the sterilizer exceeds the maximum withstand pressure, the air release valve will automatically pop open, and the contents of the pot cannot be guaranteed to be sterilized completely, so it needs to be sterilized again. That's all I have to think of, I hope it helps you!

Because of different nutrients, the kinetics of their degradation are different.

Sterilization. It refers to the use of strong physical and chemical factors to make all microorganisms inside and outside any object permanently lose their ability to grow and reproduce. The commonly used methods of sterilization include chemical reagent sterilization, radiation sterilization, dry heat sterilization, moist heat sterilization and filter sterilization.

According to different needs, different methods can be used, such as medium sterilization, which is generally sterilized by moist heat, and air is sterilized by filtration.

The thoroughness of sterilization is limited by the sterilization time and the strength of the sterilant. The resistance of microorganisms to sterilants depends on the density of the population originally presented, the resistance given to the culture by the culture, or the environment. Sterilization is an essential condition for obtaining pure cultures.

It is also an essential technology in the food industry and in the pharmaceutical field.

Basic Requirements: Reused diagnostic and treatment instruments, utensils and articles should be cleaned before disinfection or sterilization after use.

Prion, gas gangrene.

and the sudden contamination of infectious disease pathogens with unknown causes, such as diagnostic and therapeutic apparatus, appliances and articles, shall be subject to the provisions of Chapter 11 of WS T 367.

Heat- and moisture-resistant surgical instruments should be sterilized by pressure steam, and chemical disinfectants should not be used.

Immersion sterilization. The environment and the surface of objects should be cleaned first and then disinfected; When contaminated by the patient's blood, body fluids, etc., the contaminants are removed first, and then cleaned and disinfected.

The disinfection products used in the disinfection work of medical institutions should be approved by the health administrative department.

Approve or comply with the corresponding standard technical specifications, and shall follow the scope, methods and precautions for approved use.

Because of different nutrients, the kinetics of their degradation are different.

That is, at the same temperature and at the same time, some components have been destroyed or changed, while some components have not been destroyed by the big stupidity.

Just as substances with unsaturated bonds are susceptible to oxidation in air, minerals are stable for a long time.

The reason for using high temperature is generally to defend against sterilization, and the bacterial wall of the bacteria, that is, the glycoprotein components, is denatured in a short period of time at high temperature to kill bacteria. And as much as possible, other ingredients are protected from change.

It's a compromise.

After autoclaving, the changes that will occur in the culture of Limb Wookie are ().

a.Precipitation may occur.

b.The nutrient composition changes, and the color ridge deepens.

Change. d.It may not solidify after sterilization of the culture or nutrient base.

Correct answer: ABCD

Generally, the conditions for the medium to roll over the autoclave sterilization are ().

Correct answer: 121°C for 15-30min

1. The water under the water baffle should be sufficient.

2. In order to ensure safety, the exhaust should be discharged to below zero before opening the cover.

3. The water under the baffle should be pure water, and it should be changed frequently.

How to use it correctly:

1. Check whether the water in the autoclave is enough, close the drain valve, and put the sterilized items into the autoclave.

2. Close the lid tightly and turn on the power to see if the indicators are normal, if the lid is not tightly covered, the indicator light showing the lid will light up, then you need to re-cover the lid.

3. The next step is to adjust the sterilization temperature and sterilization time.

4. Close the exhaust valve, as soon as the sterilization time comes, the autoclave will send out an alarm sound, then turn off the power immediately, and when the pressure is reduced to zero, open the exhaust valve to exhaust. (Do not open the exhaust valve at very high pressure to prevent danger from occurring).

5. When the gas is exhausted, the lid can be opened and the sterilized object can be taken out.