What is the extract when extracting plant protein? Urgently needed oh 30

PBS or other phosphate extracts can be found in this molecular cloning guide.

The preparation method of phosphate buffered saline containing Tween 20 (referred to as PBS T) is: weigh potassium phosphate monobasic (KH2PO4), sodium phosphate dibasic (Na2HPO4·12H2O), sodium chloride (NaCl), potassium chloride (KCL), Tween 20, and add water. pbs:

Phosphate Buffered Saline PBS 1L Formula: Potassium Phosphate Monobasic (KH2PO4): , Sodium Phosphate Dibasic (Na2HPO4):

Sodium chloride (NaCl): 8g, potassium chloride (KCL), add about 800ml of deionized water to stir and dissolve thoroughly, then add concentrated hydrochloric acid to adjust pH, and finally set the volume to 1L. Store at room temperature after autoclaving.

PBS buffer (NaCl 137mmol L, KCl, Na2HPO4 10mmol L, KH2PO4 2mmol L PBS buffer is generally used as a solvent to dissolve the protective reagent, and the specific reagent generally has different proportions and formulations, which has a better effect in terms of targeting. 1x PBS buffer is the PBS configuration used and can be used directly, 2x PBS is 2x the concentration and is used with a double dilution.

PBS is generally not used to prepare buffers and is used for other purposes.

Shanghai Biotech's one-step phytoactive protein extraction kit BSP004 can be used

It's called sample buffer, and you can understand it.

1. Extraction method: the simplest method, that is, the method of using mechanical instruments to apply pressure to make plant juice flow out. All citrus genera.

Plants use this method.

Extraction method: A method that uses a solvent to dissolve the essential oil, and then separates the solvent to obtain pure essential oil products. Due to the nature of essential oils, it can be divided into three methods:1General solvent extraction.

Most essential oils are used in this way, using non-polar solvents, such as petroleum ethers.

toluene, etc. 2.Resin extraction method: essential oils with high boiling point are difficult to volatilize, and the general extraction method will be a great waste of heating energy, so this method uses resin as a solvent. 3.Carbon dioxide.

Extraction: This method is mandatory for essential oils that are sensitive to heat. 3. Distillation method:

There are two methods of dissolving essential oils by using water vapor: direct and indirect: 1Direct Steam Distillation:

The most common method of refining essential oils is the method of blowing steam directly from the bottom of the distillation column into direct contact with the extracted plants. 2.Indirect steam distillation:

The extracted plant is heated to a boiling with water. In addition, some essential oils need to be refined to be purer after refining, or those whose color turns black, they can be refined by vacuum distillation, and the steam is only used for heating, and is not in contact with the essential oil.

1. Principle of salting-out method: salting-out method refers to the method of adding a large number of inorganic salts to the pharmaceutical solution to reduce the solubility of some polymer substances and precipitate them, and separate them from other components. The salting out method is mainly used for the separation of proteins, pure or bending.

The inorganic salts commonly used for salting out include sodium sulfate, magnesium sulfate, ammonium sulfate, etc. 2. Principle of organic solvent method: the main reason for protein precipitation caused by organic solvent is that the dielectric constant of the aqueous solution is reduced by adding organic solvent, which increases the attraction between the two collision countercharge groups and promotes the aggregation and precipitation of protein molecules.

Another explanation for protein precipitation caused by organic solvents is similar to salting out, in which organic solvents compete with proteins for aging water, resulting in protein dehydration and easy to form precipitation. 3. The principle of isoelectric point method: at the isoelectric point, the protein molecule exists in the form of zwitterionic ions, and its molecular net charge is zero (that is, the positive and negative charges are equal), at this time, the protein molecular particles in the solution do not have the same charge to repel each other, the force between the molecules is weakened, and the particles are very easy to collide and condense to produce precipitation, so the protein has the smallest solubility at the isoelectric point, and it is most likely to form a precipitate.

Many of the physical properties at the isoelectric point, such as viscosity, expansion, osmotic pressure, etc., are reduced, which facilitates the filtration of suspensions.

Your question is very general and mainly divided into the following types:

1.Pressing, such as oil extraction and juice extraction, is the extraction of juice.

2.Extraction is the use of a certain solution to dissolve the plant juice, such as extracting some traditional Chinese medicine ingredients.

3.Distillation is mainly to extract aromatic hydrocarbons in the juice, such as extracting essential oils and so on.

Plant extract is a product formed by using plants as raw materials, according to the needs of the use of the extracted final product, through the process of physical and chemical extraction and separation, to obtain and concentrate one or more active ingredients in the plant without changing the structure of the active ingredients. According to the different components of the extracted plants, glycosides, acids, polyphenols, polysaccharides, terpenes, flavonoids, alkaloids, etc. are formed; According to different properties, it can be divided into vegetable oil, extract, powder, lens, etc.

Aqueous extract is an extract formed by dissolving and extracting water as a solvent and concentrating it.

Plant extracts with water as the extraction solvent.

Xi'an Aoze Biotechnology****.