Microscope Operations and Techniques 20

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Get ready. The instrument should be placed on a cool, dry, dust-free, acid-alkali, and steam platform, and the dust cover should be covered when not in use.

Operate. 1.Place the specimen to be observed on the stage and clamp it on the clip.

2.Each magnification objective is mounted on the nosepiece, and the eyepiece is inserted into the eyepiece tube.

3.Move the specimen to the middle of the stage and observe with a low-magnification objective first. Turn on the power switch to move the brightness adjustment button to the appropriate position, turn the coarse adjustment handwheel to raise the stage to the shadow of the specimen where you can see it, and turn the fine adjustment handwheel to get a clear image.

The brightness option is to turn the condenser handwheel to raise or lower the condenser, and then adjust the variable bar to change the aperture of the light bar to obtain illumination brightness suitable for a variety of detailed specimens. (According to the observation needs, the color filter is prepared for use, and the color filter is mounted on the bracket at the lower part of the variable light bar to obtain the selected color.) ）

Rotate the longitudinal handwheel on the stage to move the specimen in the forward and backward directions; Rotate the transverse handwheel on the stage to move the specimen in the left and right directions. Move the object to be observed to the center for observation, and then transfer to the high-magnification objective or oil immersion objective for observation (cedar oil should be added to the specimen observation area when using the oil lens).

When changing the observation (the objective lens does not touch the sliced object), the image of the object can still be seen, and the fine adjustment handwheel needs to be turned again to achieve a clear image.

After use, just turn the coarse adjustment handwheel to lower the workbench to the bottom, then move the brightness adjustment button to the minimum brightness, and finally turn off the power switch.

4.Adjust the brightness adjustment knob to change the luminous brightness of the multi-sardine bubble to get the best brightness.

5.How to replace the bulb: unscrew out the knurled screws, turn the lamp base plate over, remove the bulb, and replace it with a new bulb.

6.Loading and unloading of the light collector and light source adjustment method: when using an artificial light source, aim the light collector at the screw on the base, rotate the light collector clockwise to the end to make it tight, then lift the microscope slightly, loosen the adjustment screw under the base, adjust the filament image to the center of the field of view, and then tighten the adjustment screw for observation under the best lighting.

7.When you are done, turn the coarse adjustment handwheel to lower the workbench to the bottom, then move the brightness adjustment button to the minimum brightness, and finally turn off the power switch.

Here's how to use the microscope:1) First of all, under the low magnification, aim at the light source, and adjust the anti-spring bright noise mirror to be the brightest in the field of view.

2) Then place the specimen slide in the stage.

3) Then lower the tube to the slide as close as possible.

4) Continue to lower the light collector or turn down the light grill circle.

5) Slowly raise the tube with coarse adjustment until you can see the key tan specimen.

6) Finally, change to a high magnification lens, magnify the aperture and ascend the light collector, and adjust the fine adjuster until you can see clearly.

Here's how:

1. Take the mirror and place it.

Hold the temple arm with your right hand and hold the lens base with your left hand. Place the microscope about 7 cm from the edge of the bench, slightly to the left, and attach the eyepiece and objective.

Second, to the light. Turn the converter so that the low-magnification objective is aligned with the clear aperture (keep the front of the objective 5 to 10 mm away from the stage). Aim a larger aperture at the aperture with one eye looking at the eyepiece and the other eye open.

Rotate the mirror so that the light is reflected into the lens barrel through the aperture, and the white circular field of vision can be seen through the eyepiece.

3. Observation. Place the slide specimen to be observed on the stage and press it with a clamp facing the center of the aperture. Turn the coarse collimation spiral so that the lens barrel is slowly lowered until the objective lens is close to the slide specimen.

One eye looks inside the eyepiece, and at the same time rotates the coarse quasifocal spiral in a counterclockwise direction so that the lens barrel slowly rises until the object is clearly visible. Then turn the fine collimation spiral slightly to make the object more clear.

Fourth, change the high magnification lens.

After you have seen clearly under low magnification, move the image of the object to be magnified to the field of view**.

Turn the converter and change to a high-magnification objective. Observe and focus with a fine-collimating spiral.

Note: 1. Before using the high-magnification objective, you must first use the low-magnification objective lens to find the observed object, adjust it to the positive ** of the field of view, and then turn the converter and change the high-magnification lens.

2. After switching to a high-magnification lens, the brightness in the field of view becomes darker, so a larger aperture is generally selected and the concave surface of the reflector is used, and then the fine collimation spiral is adjusted. ** The number of objects is smaller, but the volume is larger.

3. The magnification of the microscope is the eyepiece magnification multiplied by the objective lens magnification, such as the eyepiece is 10 times and the objective lens is 40 times, the magnification is 40 10 times (magnification 400 times), and the ultimate magnification of the optical microscope is 2000 times.

The use of the microscope and the steps are as follows:

1. Take and place When using, first take out the microscope from the lens case, you must hold the lens arm with one hand, hold the lens holder with the other, keep the lens body upright, and never use one hand to tilt and carry it to prevent the eyepiece from falling. Handle with care, with the temples facing you, 5-10 cm from the edge of the table. The table is required to be balanced, the top is clean, and it is protected from direct sunlight.

2. Turn on the light source and turn on the power switch.

3. Place the slide specimen Place the slide specimen to be examined on the stage, so that the material is facing the light aperture of the escort lifter**. The slide is then clamped at both ends with a spring pressure to prevent the slide specimen from moving. In the case of a slide movers, snap the slide specimen into the slide mover, then adjust the slide mover to move the material directly opposite the clear hole**.

4. Low magnification objective observation When observing specimens with a microscope, the object image should be found with a low answer chain objective lens first. Because of the large observation range of the low-magnification objective, it is easier to find the object image and find the parts that need to be carefully observed. Its method is like a reed arguing:

1) Turn the coarse adjustment spiral and look from the side with your eyes to lower the lens barrel until the low magnification objective lens is about a centimeter away from the specimen.

2) Observe from the eyepiece with your left eye, open your right eye naturally, and slowly turn the coarse adjustment spiral with your hand so that the lens barrel gradually rises until the image in the field of vision is clear. After that, the fine-tuning spiral was used, and the focal length was slightly adjusted to make the image the clearest.

3) Gently move the slide forward, backward, left and right with your hand or adjust the slide mover to find the part you want to observe. It should be noted that the object in the field of view is inverted, and the slide should be moved in the opposite direction.

1. "Take the mirror and place it in the fierce mountain.""Hold the "mirror wall" with your right hand and hold the "mirror base" with your left hand, and take out the microscope flat and put it in a suitable position.

2. "Installation.""to remove the "dust cover."", twist the "coarse quasi-focal spiral.""will"Lens tube"Elevated, use a "10x objective.""Installed in the "Zhi Roll Converter."", align the converter with the "clear hole.""Centre.

3. "Align the light", turn the "reflector", and adjust it to the "eyepiece" to see a very bright field of vision.

4. "Install the standard" and put the sample in""The stage", so that the standard in the clear aperture **.

5. "Observation record", the left eye looks at the eyepiece, the right eye is opened, and the hands twist and turn the spiral until you see a clear image of the standard product and record it.

6. "Tidy up the microscope."", take out the sample solution, put the eyepiece and objective lens back in place, screw the lens barrel to the lowest and cover it with a dust cap, adjust the mirror to be parallel to the tabletop, wipe the microscope clean, and put it back in the same way.

The above is provided by Linktour Instruments.

1 Hold the arm with your right hand and hold the base with your left hand.

2 Place the microscope on the bench, slightly to the left (the microscope is about 7 cm away from the edge of the bench). Attach the eyepiece and objective.

Second, to the light. 3 Turn the converter so that the low-magnification objective is aligned with the clear aperture (keep the front end of the objective at a distance of 2 cm from the stage).

4 Align a larger aperture at the aperture. The left eye is fixed inside the eyepiece (the right eye is open so that the picture can be drawn at the same time later). Turn the mirror so that the light is reflected into the barrel through the aperture. Through the eyepiece, you can see a white and bright field of vision.

3. Observation. 5 Place the slide specimen to be observed (it can also be made from a thin piece of paper with the word "6" printed on it) on the stage and press it with a tablet clamp, and the specimen should be facing the center of the light hole.

6 Turn the coarse collimator spiral so that the lens barrel is slowly lowered until the objective lens is close to the slide specimen (eyes look at the objective lens so that the objective lens does not touch the slide specimen).

7 The left eye looks into the eyepiece and at the same time turns the coarse collimation spiral in the opposite direction so that the lens barrel slowly rises until the image is clearly visible. Then turn the fine collimation spiral slightly to make the image of the object seen more clearly.

<>1. Adjust the brightness: from dark to bright, you can use a large aperture and a concave mirror to adjust the angle of the reflector.

2. Fix the temporary loading god on the loading table in an appropriate position.

3. The low-magnification objective lens is aligned with the clear hole, and the lens barrel is adjusted from top to bottom by using a coarse quasifocal spiral, so that the eyes can observe on the side of the circular surface to avoid the objective lens from touching the slide and damaging the lens and crushing the slide.

4. The left eye observes the changes in the visual field through the eyepiece, and at the same time adjusts the coarse quasifocal spiral to slowly move the lens barrel up until the visual field is clear.

5. If there is no object to be observed in the field of vision, you can move the film, the principle is to want to go up and down, and want to go left and right.

6. If it is not clear enough, it can be further adjusted with a fine collimation spiral.

Steps for using an optical microscope:

1. Take the lens and place it.

Hold the temple arm with your right hand and hold the lens base with your left hand.

Place the microscope on the bench and mount the eyepiece and objective lens slightly to the left

2. Turn the converter to align the low-magnification objective with the clear aperture Note that the front end of the objective lens should be kept 2 cm away from the stage

Aim a large aperture at the aperture with your left eye inside the eyepiece and your right eye open for later viewing and drawing Turn the mirror to see a bright field of vision

3. Observation Installation and loading: Put the glass slide to be observed on the stage, press it with a tablet clamp, and the specimen should be facing the light hole

Focusing: Rotate the coarse collimation spiral to make the lens barrel slowly descend, and look at the objective lens to avoid the objective lens touching the slide specimen until the objective lens is close to the glass slide At this time, the left eye looks into the eyepiece, and at the same time rotates the coarse collimation focus in the opposite direction, so that the lens barrel slowly rises until the object image is found, and the object image is not clear, and then adjust the fine collimation spiral to make the object image clearer

Finally, the laboratory table is finished

To sum up, the correct steps to use a microscope should be: take the lens, place it, align it with light, observe, and close the lens

Precautions for use of the microscope and its use.