What are the similarities and differences between the principles of ordinary light microscopy and fl

Similarities:1All are optical microscopes;

2.Both qualitative and quantitative analysis of biological samples can be performed.

Differences:1The light sources used in the two are different: ordinary optical microscopes use natural light, while fluorescence microscopes mostly use ultraviolet light;

2.Fluorescence microscopes contain a special filter system;

3.Fluorescence microscopy also has the characteristics of high sensitivity and specificity.

4.Fluorescence microscopy can only use non-fluorescent glass slides and non-fluorescent oils, etc.

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Main differences: 1 Fluorescence microscopy. The illumination method is usually epiphotogenic, that is, the light source is projected onto the sample through the objective lens;

2.The light source is ultraviolet light, the wavelength is shorter, and the resolution is higher than that of ordinary microscopes;

3.There are two special filters, in front of the light source to filter out visible light, and between the eyepiece and the objective lens to filter out ultraviolet rays to protect the human eye.

The LW300LFT fluorescence microscope is also a type of optical microscope, the main difference is that the excitation wavelength of the two is different. This determines the difference in structure and use method between fluorescence microscope and ordinary optical microscope.

Fluorescence microscopy is an essential tool in immunofluorescence cytochemistry. It is composed of the main components such as the light source, the filter plate system and the optical system. It is the use of a certain wavelength of light to excite the specimen to emit fluorescence, and the fluorescence image of the specimen is magnified by the objective lens and eyepiece system.

Dark-field microscopy.

The background of the image I saw was dark (I only saw the shape of the cell during the experiment, but I didn't see the nucleus...). The cell wall is bright, and the contrast between light and dark is obvious. The inside of the cell wall is dark and the observed cells are opaque.

Phase contrast microscopy.

See the follow. Light microscopy.

Under almost, because will.

The phase difference is transformed into an amplitude difference, and the unstained onion epidermis is clearer and brighter, and the nucleus can be clearly seen.

Fluorescence microscopy.

Since there is no chlorophyll in the epidermal cells of the onion, it is generally necessary to observe the secondary fluorescence emitted by the nucleus clearly after staining observation!

Hope it helps

A darkfield microscope is a type of microscope that uses the Tyndall effect to create a strong contrast between the observed specimen and the background. It can be used to observe tiny living bacteria and their movement state.

Phase contrast microscopy is a microscope that uses the diffraction and interference phenomena of light to convert the optical path difference or phase difference of light passing through the specimen into an amplitude difference microscope that can be distinguished by the naked eye. Improves the distinction between light and dark images of different densities and can be used to visualize unstained cell structures.

A fluorescence microscope is a microscope that is specially designed or equipped with an additional device for fluorescence microscopy. It is also possible to measure semiconductors, LCDs, and other products.

Let's see what others have to say.

Fluorescence, a Chinese word. Also known as "fluorescence", it refers to a photoluminescent cold luminescence phenomenon.

Fluorescent substances such as HFP protein, some components in asphalt, even spinach also have fluorescent substances, zebrafish, etc., so fluorescence microscopes are widely used in industry, medicine and other industries.

Fluorescence microscope has a light source emitted by a fluorescent light source, and a part of the wavelength of light after passing through the cut-off piece is irradiated on the surface of the object through the objective lens, and the object with fluorescent characteristics or the object dyed by fluorescent dyes excites fluorescence, and then the light wave that can be observed through the cut-off piece enters the eyepiece and is observed.

A fluorescence microscope is also a microscope with a fluorescence device added to an ordinary microscope. Fluorescence has a shorter wavelength, so an objective fluorescence microscope that can also observe fluorescence has a higher resolution.

Fluorescence microscopy for the observation of GFP proteins in immunofluorescence is a typical application example.

Eyepiece. The eyepiece is also the main component of the microscope, and its main function is to magnify the real image obtained by the objective lens again, so as to form a clear virtual image at the photopic distance; Therefore, its quality will ultimately affect the quality of the image. In photomicrography, a real image is formed at the ground glass.

Some eyepieces, such as compensation eyepieces, can correct for residual aberrations generated during objective imaging in addition to magnification. The construction of an eyepiece is much simpler than an objective. Because the beam passing through the eyepiece is close to parallel, spherical aberration and longitudinal (axial) chromatic aberration are not severe.

Only transverse chromatic aberration (magnified chromatic aberration) is considered in the design.

Correct answer: c

Analysis: The light source, objective lens, filter plate, and condenser of a fluorescence microscope are different from those of ordinary microscopes.

Answer] The light source, objective lens, filter plate, and condenser of :d fluorescence microscope are different from those of ordinary microscopes. Fluorescence microscope uses ultraviolet light as the light source, and the wavelength is shorter, and the resolution is higher than that of ordinary microscopes.

Among the structures that make up the fluorescence microscope, only the potato leak eyepiece is the same as that of a normal optical microscope.

The resolution ability of the electro-reed elimination microscope is that of the ordinary optical microscope before the stupidity ().

Fold. Fold. Fold. Fold.

Correct answer: c

Answer]: The working principle of the laser scanning confocal microscope is to use the laser scanning beam to irradiate the point light source formed through the illuminated pinhole, and scan each point of the focal plane in the specimen, and the irradiated point on the specimen is imaged at the detection pinhole. Most fluorescein requires short-wavelength excitation, and ultraviolet light is used as the excitation source.

Ordinary light microscopes use visible light as the light source.

Answer]: A several kinds of microscopic silver mirrors commonly used light sources, such as ordinary optical microscopes often use natural light extinction as the light source, fluorescence microscopes use ultraviolet light as the light source, and electron microscopes use electron fluorescence instead of light sources.

Similarities: 1) both are optical microscopes; 2) Both qualitative and quantitative analysis of biological samples can be performed.

Different internal points: 1) The light source used in the two is different: ordinary optical microscopes use natural light, while fluorescence microscopes mostly use ultraviolet light; 2) Fluorescence microscopes contain a special filter system; 3) Fluorescence microscope also has the characteristics of high sensitivity and strong specificity; 4) Fluorescence microscopes can only use non-fluorescent glass slides and non-fluorescent oils, etc.