What is the difference between in vivo and in vitro cell culture in terms of nutritional requirement

In most cases, cells need serum for in vitro culture, but the serum contains exosomes, in order to avoid exosomes produced by serum contamination cells, two methods are generally used: Before cell culture, through 100,000??Ultracentrifuge overnight to remove serum exosomes.

Serum-free medium can be selected for culture. Yumeibo Biotech is providing free exosome extraction kits on a first-served, first-served basis.

There is a difference in nutrient metabolism between isolated cells and cells in vivo. Cellular nutrition in the body can be regulated by nerves and hormones, while isolated cells are not regulated by it. The main differences in nutrient requirements between cultured cells in vitro and in vivo cells are as follows:

Most cells cultured in vitro for a long time require plasma, serum, or embryo leachate, and such media may contain trace amounts of hormones, vitamins, and essential amino acids to provide nutrients for the cells.

In the body, many highly active substances in the blood only need a small amount to maintain the nutritional needs, and can not be observed in vitro culture, the amount of more may be different from the effect of the micro, the amount of less may be quickly consumed.

Cultured cells are a group of isolated cells that have different nutritional requirements. These different nutrient requirements are indirectly supplied in the body by the activities of cells in other organs and tissues, whereas there is no such supply in the culture medium and is not affected by the metabolism of other tissues.

All kinds of tissues have their own special nutritional requirements, which can be suitable for the culture medium of one tissue, but not necessarily suitable for the cells of other tissues, and the difference is sometimes large.

In vivo cells, their regeneration and decay maintain a balance, while in vitro culture, cells proliferate, increase in size, proliferation rate is often higher than differentiation rate, new cells grow faster than decaying cells, so the nutritional requirements are naturally different.

It can be seen that different types of cells have different nutritional requirements. Therefore, the study of cell nutrition is very important in tissue cell culture. All substances that can enter the cell and be used by the cell, participate in the metabolic activities of the cell and maintain the survival of the cell are nutrients.

In addition to the three major nutrients of sugars, amino acids and lipids, a certain amount of inorganic salts, vitamins and trace elements are also required for the growth of cells cultured in vitro.

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In vivo cell culture is performed directly in the abdominal cavity of the animal, and in vitro cell culture is performed in vitro with the addition of animal serum. The main ingredients should be the same in the body, and it is not easy to be exactly the same.

The most important culture material is animal serum (because it contains antibodies) and the environment is a carbon dioxide incubator (where carbon dioxide plays a role in regulating pH, because the pH of the medium changes during the culture process, which is not conducive to cell reproduction).

Primary cells refer to cells that are obtained from human tissues (such as human tissues, mouse tissues, rat tissues, rabbit tissues, etc.) by protease or other methods and cultured in vitro to mimic the body, which is called primary cells. It is generally believed that cultured primary first-generation cells and cells that have been passed up to the 10th passage are collectively referred to as primary cell cultures. The survival, growth, reproduction and passage of primary cells under artificial conditions are carried out to study the cell life process, cell carcinogenesis, cell engineering and other issues.

A cell strain is a cell line in which special properties or markers are obtained from primary cultured cells by selection or clone formation. It is generally believed that cell lines are cell populations formed by single-cell proliferation using single-cell isolation culture or by screening methods. The special properties or hallmarks of the cell line must be present throughout the culture period.

A cell line is a cell line in which primary cells have been successfully passaged for the first time. Refers to cells that are generally likely to be passaged. Among them, the cells that can be passed continuously are called continuous cell lines or infinite cell lines, and those that cannot be continuously cultured are called finite cell lines.

Most diploid cells are limited cell lines. It is composed of a cell lineage that was originally present in the primary culture stool. If the passage can be continued, or the number of passages is limited, it can be called a finite cell line, and if it can be cultured continuously, it is called a continuous cell line, which can be cultured for more than 50 generations and continued indefinitely.

Human tumor cells that have been cultured in vitro for more than half a year, grow stably, and are passed continuously can be called continuous strains or lines.

The temperature is 37, the humidity is 25 65, the carbon dioxide is about 5%, and the key is the aseptic treatment of the environment.