What is hirudin used for? How is hirudin extracted?

Hirudin is a small molecule polypeptide substance extracted from the salivary glands of blood-sucking leeches, with a molecular weight of about 7000 daltons, but according to the different types of blood-sucking leeches, the molecular weight extracted is also different.

The people who used to be in the fields should have been impressed, the kind of mollusks that suck blood on their legs, and if they are bitten, they can use salt to loosen their mouths.

Function: It is mainly anticoagulant thrombolysis, targeting the blood, preventing and treating blood vessel blockage, accelerating metabolism, and taking away vascular garbage, so many of them are now used in cardiovascular and cerebrovascular diseases, such as: cerebral infarction, myocardial infarction, atherosclerosis, coronary heart disease, etc.

Because of the small amount of analysis, it has a good penetration effect, and it is a protein itself, so it is now also used in skin care products.

Hirudin is used to protect anticoagulant and antithrombosis, you can eat hirudin, nattokinase content is very high, mainly this is good.

1. Traditional methods.

Since natural hirudin is present in the saliva, glands and saliva secreted by blood-sucking leeches, the traditional method of extracting natural hirudin is usually to extract natural hirudin from the head or whole body of the Japanese leech.

The conventional extraction method is to use the head of the blood-sucking leech as the raw material, because there is a large number of "pseudo-hirudin" components similar to hirudin in the body of the leech, but there are no bioactive "pseudo-hirudin" components, and the storage of food increases the difficulty of hirudin separation.

The crude solution of hirudin is obtained by extracting with water-acetone mixture or by ethanol fractionation and precipitation, and then the natural hirudin is extracted by chromatography, isoelectric focusing and other methods.

2. Biomimetic induction method.

Biomimetic induction method refers to a method of inducing live leeches by the method of biomimetic induction, so as to obtain leech culture. It avoids the one-time killing of leeches by traditional methods, not only protects the wild resources of leeches, but also promotes the development of leech aquaculture industry, and provides a theoretical basis for the industrialization of hirudin.

The specific method is to starve the Japanese leeches under oxygenated conditions for a week, and then use pig blood and snails to mix the induction system at 25, pH 72 conditions were induced to secrete saliva for 30 min.

The saliva is then further separated and purified to obtain natural hirudin. This is a revolution in the field of traditional extraction of natural hirudin, which does not kill leeches, but can be used later, and reduces the extraction of water-acetone or ethanol fractionation and precipitation in the separation and purification process, which is simple to operate and unique in method, and significantly reduces the production cost, so that the large-scale production of natural hirudin becomes a reality.

Innova's unique method of isolating and purifying hirudin has a simple process, low cost, high extraction rate, and high purity and activity of hirudin. The active protein isolated and purified from natural locust powder has an activity of up to 13000 atu g.

As one of the three major elements in the world, hirudin has great value. In recent years, hirudin has also been used in many ways in skin care. There are 4 main aspects of hirudin's efficacy in skin care.

1.Small molecule, strong autonomosis.

Scientific research has invented that human cells can only absorb substances with a molecular weight of 8000 daltons under normal circumstances, while hirudin has a very small molecular weight, only 7000 daltons, which can directly and quickly penetrate into the deep layers of the human body and is easy to be absorbed and utilized by cells.

2.Promotes blood circulation and repairs acne scars.

Hirudin can promote blood circulation and also has a certain effect on scar repair. Acne scars are formed after acne, **inflammation causes telangiectasia, and after the acne is eliminated, the blood vessels do not shrink and are formed. Therefore, the function of hirudin to promote blood circulation is very targeted to the red acne scars left by acne.

3.Repair cells and nourish skin.

Hirudin is a small molecule polypeptide substance, which has the properties of a protein but is simpler than a protein. It has a small molecular weight, is easily absorbed, and has high activity. It can help to repair cells and replenish nutrients to the skin.

4.Effectively aids osmosis and promotes absorption.

In addition to its strong direct and independent permeability, hirudin can also help other substances to penetrate, penetrate deep into the skin, promote ** absorption, and have rare permeability.

Leech was first included in the Shennong Materia Medica), which is a classic blood-breaking and stasis medicine. Here are the five most obvious effects of hirudin that I have brought to you, let's take a look.

1. Dissolve thrombosis.

2. Eliminate blood garbage.

3. Reduce blood viscosity.

4. Improve blood circulation.

5. Promote metabolism.

Name of medicinal herb: leech.

Pinyin: shuǐ zhì

Aliases: locust, turtle, meat drill.

Properties: Leeches and leeches are dry and whole. Leeches are oblong cylindrical, composed of many links, the body is mostly curved and twisted, 2 to 5 cm long and to cm wide.

The back is dark green or black-brown, the ventral surface is gray-green, the body is light and brittle, easy to break, the section is colloidal, uneven, dull, and slightly fishy. Leeches are large, flattened spindle-shaped, 4 to 10 cm long and 2 cm wide. dorsal black-brown or black-brown, slightly raised, black spots arranged in 5 longitudinal stripes after flooding; The ventral surface is flat, brownish-yellow or brownish-yellow on both sides, the front end is slightly pointed, the posterior end is blunt and rounded, each end has a sucker, the rear sucker is large, it is obvious, brittle, easy to break, the section is gelatinous, shiny.

It is better to be small, neat and black-brown.

Chemical composition: Fresh products contain hirudin, which is the secretion of the head gland of leeches, and is a polypeptide. But in dried crude drugs, hirudin has been destroyed. In addition, its secretions contain a histamine-like substance, heparin and antithromboxane.

Sexual taste: salty, bitter, flat; There is a small poison.

Return to the meridian: return to the liver meridian.

Storage: Put it in a dry place to prevent moths.

Contraindications: Weak and blood-deficient, pregnant women, women during menstruation and those with bleeding tendencies are contraindicated.

Internal administration: into pills, scattered, money. --Dictionary of Traditional Chinese Medicine

External use: sucking at the diseased area; or leaching droplets. --Dictionary of Traditional Chinese Medicine

Oral:. - Chinese Pharmacopoeia

Hirudin has many roles in medicine, mainly including:

1. Anticoagulation and thrombolysis;

2. Dilute blood and reduce blood viscosity;

3. Remove blood garbage;

4. Inhibit the aggregation of red blood cells and platelets;

5. Protect the intima of blood vessels;

6. Dilate and soften blood vessels;

7. Anti-inflammatory and analgesic;

8. Inhibit tumor growth;

9. Freckle removal and beauty.

10. Remove cholesterol and grease garbage.