High School Biology Animal Cell Culture, High School Biological Cells?

At first, the bottle should be divided in the re-culture, and, generally, there is a kind of cell culture apparatus (a kind of cell culture apparatus, it comprises a rotor that can hold a plurality of cell culture vessels and rolling flasks separably, is equipped with the device that makes the rotor rotate around a basic horizontal axis at a controlled speed for the purpose of cell culture, is also equipped with the device that makes the rotating shaft of the rotor of the apparatus and the flask turn to the basic vertical position, so that a cap end of the culture flask is at the bottom, and each culture flask is equipped with a cap, described bottle cap is equipped with a fluid supply discharge connector, when described culture flask is upside down, bottle cap is at the bottommost edge, described fluid supply discharge connector is arranged at the lowest point of bottle cap, a manifold has one or more sealable external connectors and the connector that is connected with the fluid supply discharge connector of each bottle cap, the gas space in the culture flask is ventilated through a breather pipe during fluid conveyance, and the vent pipe passes through the fluid of inverted position upward, forms an internal extension section of bottle cap, the ventilation pipe extends into the bottle body, the ventilation pipe has one end that leads to the bottle body, is in the position of no fluid in the culture flask when the culture flask is basically vertically or horizontally oriented, the ventilation pipe is also equipped with the microporous ventilation part of the air ventilation, and the component is arranged to carry out fluid transportation to or from the culture flask through the manifold external connector when the rotor and the culture flask are in the position of basically vertical inversion. You don't need to be able to count people to determine the number of dispenses.

There is not so strict how many generations are divided, how many generations are passed on, those are all theoretical data. In the actual operation, it is to see if the cells are overgrown, by judging the color of the medium (with an indicator), and from the inverted microscope, the color has changed, and the cells under the microscope are already dense.

That's when it's time to split the bottles. Before the first bottle separation, the primary culture is entered, and as long as the bottle is divided, the subsequent ones are subcultured. Pure hand hitting, looking.

10 generations to 50 generations, that is, you need to pass, as for whether to divide the bottle depends on your needs, if you just want to confirm the continuity of the cell line, karyotype, etc., you can not pass all the passaging, one bottle at a time is enough (to ensure that the density of the passage is not too large), you do not pass once is +1

Separate flasks are required, contact inhibition occurs during the culture process, and cells cannot continue to grow without flasking.

1. Correct. In cells with vigorous secretion protein synthesis, the endoplasmic reticulum membrane should continuously bulge out vesicles to wrap secreted proteins and transport them to the Golgi apparatus.

It is transformed into the Golgi membrane, which continuously bulges out vesicles to wrap secreted proteins and transport them to the cell membrane.

The transformation into cell membranes is noisy, so the renewal rate of Golgi membrane components is rapid.

2. Correct. The transmission of excitation between neurons relies on neurotransmitters.

information molecules) bind to specific receptors on the postsynaptic membrane (the cell membrane of neurons) to cause excitation or inhibition of the postsynaptic membrane, reflecting the functional characteristics of the cell membrane for information exchange.

If you don't understand Jane, please ask, satisfied, thank you! Hope it helps

It is clear that the Golgi apparatus is involved in the transport of secreted proteins, so when the endocrine protein synthesis is vigorous, the renewal rate of the basal body membrane is fast.

Correctly, excitation is transmitted between neurons, relying on a neurotransmitter, a cellular secretion that needs to bind to another neuron through endocytosis and efflux, and this process is done by the cell membrane.

All right. 1. Secreted proteins need to be processed by endoplasmic reticulum and Golgi apparatus, and secreted from the cell membrane to the outside of the cell. The endoplasmic reticulum is transported to the Golgi apparatus through vesicles, and the Golgi apparatus transports the wide cell membrane through the vesicles, so the membrane of the Golgi apparatus has always been updated relatively quickly.

2. When excitation is transmitted between two neurons, neurotransmitters are released by the presynaptic membrane and act on the receptors of the postsynaptic membrane, which is a form of information transmission between cell lesions, and the transmission of this information is dependent on the cell membrane, so it is also correct.

Both questions are correct.

1. The Golgi apparatus undertakes the transport of proteins to the outside of the cell for the first purpose of eggplant, and the cells that "secrete proteins" mentioned in the question are the release of proteins, of course, a large number of Golgi apparatus are required to undertake the transport work. At the same time, the Golgi apparatus is made of membranes, so the membrane renews quickly.

2. The conduction of excitation between cells is completed by the induction of the fibrillation potential between membranes, and "excitement" itself is an information deficit, so this question is also correct.

This is because the role of vacuoles is to regulate the osmotic pressure of plant cells, which is beneficial in maintaining the normal morphology of the cells.

When the external solution is higher than the cell fluid, the osmotic pressure of the vacuole is less than the cytoplasmic osmotic pressure, and the cell loses water and the plasmo-wall separation occurs.

Because the water will always automatically go from the high concentration side to the low concentration side. Whereas, the concentration of vacuoles is less than that of cytoplasm. As a result, there will be a vacuole osmotic pressure that is less than that of the cytoplasm.

Cell water loss is a dynamic process, the original state is that the concentration of cytoplasm is equal to the concentration of cell fluid, when the concentration of external solution is higher than the concentration of intracellular, the cytoplasm loses water first, which destroys the equilibrium, and then leads to the concentration of cytoplasm greater than the concentration of cell fluid, and then causes water loss of cell fluid.

The cytoplasm is in the outer layer, the cytoplasm loses water first, and the osmotic pressure rises, so the osmotic pressure of the vacuole is smaller than the cytoplasm at this time. (Normally, the osmotic pressure of the two is equal).

Because the cell loses water, water flows from the vacuole to the cytoplasm, and water flows from a place with a high concentration (low concentration of solution) to a place with a low concentration (high concentration of solution), so the osmotic pressure of the vacuole (concentration of solution) is low.

aa*aa=aa aa aa aa (is a recombination of four letters) Then aa:aa:aa=1:2:1

The meaning of the question should be that there are only AA and AA and it is 2:1, which means that AA dies in the embryonic period, homozygous and dies, which means that when the genotype is AA or AA, the fertilized egg will not develop or the embryo will die in the early stage, and heterozygous will die, that is, when the genotype is AA, the zygote will not develop or the embryo will die in the early stage, whether it is heterozygous and killed, I don't know, this mainly depends on the question stem.

Choose D, the organelles of the double membrane have chloroplasts and mitochondria, animal cells have no chloroplasts, and the nuclear membrane of the nucleus is also a double membrane structure, so choose D

Whether DNA in the cytoplasm carries genetic information or is passed on to the next generation.

Yes, but generally only the cytoplasmic DNA of the mother will be passed on to the offspring, and the cytoplasm of the egg cell will be passed on to the offspring.

Ribose What is the relationship between deoxyribose and five-carbon sugar.

Ribose and deoxyribose are both five-carbon sugars. Deoxyribose is one less oxygen atom than ribose.

Gentian violet is an alkaline dye Why is it prepared Dissolve gentian violet in a solution of acetic acid with a mass fraction of 2% Isn't acetic acid acidic.

Acetic acid is a weak acid, and it does not affect the alkalinity of gentian violet after configuration.

In prokaryotic cells, can transcription and translation occur at the same time or must occur at the same time in eukaryotic cells.

No matter what cell it is, it is necessary to transcribe and produce mRNA first, and then synthesize proteins through ribosomal translation.

It's a priority.

Volume fraction Mass fraction Percentage What do they refer to?

Volume fraction is mostly used when mixing two liquids. That is, the ratio of the volume of one of the liquids to the total volume after mixing.

The mass fraction is mostly used in solute dissolution in solvents. That is, the ratio of the mass of the solvent to the total mass of the dissolved solution.

Percentages are a form of representation, and both of the above scores can be expressed as percentages.

Assisted diffusion can active transport be said to pass through several layers of cell membranes.

OK. However, endocytocytosis is not counted as crossing the cell membrane, i.e., passing through layer 0 membrane.

The concept of cross-inheritance.

To put it simply, the mother is diseased and the father is normal, and the offspring produced are normal for females and diseased for males. Vice versa.

In experiments that prove that DNA is the main genetic material, E. coli is cultured with 32p and 35s, respectively, and the E. coli's own DNA will also replicate and be radioactive in the DNA of its offspring.

This Sun Minli is lz mistaken. The 32p or 35S E. coli cultured earlier is for culturing and labeling bacteriophages.

The E. coli used in the subsequent infection experiments was unlabeled.

Antidiuretic hormone, growth hormone, glucagon and other chemical hormones are peptides, whether they can be taken orally, which can be taken orally, and which need to be injected.

None of these hormones can be taken orally and must be injected. Peptide and protein hormones cannot be taken orally, while lipid hormones, such as sex hormones, can be taken orally.

Whether the codon contained in the tall stem and the short stem of the pea is the same.

The codon table used by almost all living beings is the same.

The difference between D and D is that the genes have different bases in different order or number, resulting in different types or numbers of translated amino acids or different sequences.

Are nitrifying bacteria eukaryotes or prokaryotes.

Prokaryotes. Bacteria are all prokaryotes. Nitrifying bacteria can carry out chemosynthesis and belong to the producers.

1. Mitochondria can self-replicate and be stably inherited, and non-Mendelian inheritance.

2. Ribose and deoxyribose belong to five-carbon sugars.

A: In the photoreaction stage, water is split into [H] and O2 through photolysis of water and chlorophyll a releases a high-energy electron, and the energy in the electron and [H] can catalyze the production of ATP and Nadph; In the dark reaction, ATP is converted into ADP and PI for energy, and Nadph is converted into Nadp+[H] for energy and is a reducing agent.

B: Correct, glucose is converted to CO2 and H2O by aerobic respiration, while anaerobic respiration produces lactic acid or alcohol and CO2

C: When blood sugar is too high, the action of insulin makes blood sugar can be converted into liver glycogen, muscle glycogen, fat, etc.; When blood sugar is too low, hormones such as glucagon and fats can be used to convert the blood sugar into blood sugar to maintain blood sugar stability.

D: Can be mixed with obtained s-type bacteria.

A: In the photoreaction stage, water is decomposed into ATP [H]O2 through photolysis of water, and ATP is also generated. In the dark reaction, which violently, converts ATP to ADP and PI.

B: Correct. C: When blood sugar is too high, it can be converted into liver glycogen, muscle glycogen, fat, etc.; When blood sugar is too low, muscle glycogen and fat can be converted into blood sugar to maintain blood sugar stability. Laughing empty.

D: Can be mixed with obtained s-type bacteria.

NadP+ is converted to Nadph in the photoreactive phase and vice versa in the dark reactive phase.

Myoglycogen cannot be converted into blood finch sugar.

It is okay to use the dead one, as long as there is the nucleic acid of the S-type bacteria, it contains the mountain line.

The answer is b, the aerobic respiration product is water and carbon dioxide, the poplar respiration product has alcohol in it, or lactic acid is an organic small molecule.

The answer is b

A is wrong, the light reaction and the dark reaction are transformed.

C is false, muscle glycogen cannot be converted into glycemia.

D. Mistaken Thoughts, only the DNA of the S-type bacteria

1) Right. Generally speaking, the macro-ascending nucleus of the cell controls the instructions of the micro-soothing terrestrial organelles for material synthesis and energy conversion, which basically depends on the synthesis and synthesis speed of RNA, which enters and exits the nucleus from the nuclear pores.

2) False. The concept of a physical model is "to intuitively express the characteristics of an object of knowledge in the form of objects or pictures", which is just a **, not a physical model.

1) Yes, in gene-guided meta-protein synthesis, DNA is transcribed to form mRNA, which is then translated to form proteins. mRNA can be synthesized from the nucleus of a fine leaky ante into the cytoplasm through the nuclear pore.