What should I pay attention to when mice are gaved to death????

In the beginning, this kind of problem will definitely arise. First of all, you have to pay attention to the grasp of the mouse, it must be correct, that is, after you catch it, the struggle will not affect the degree of your gavage. Then there is the feeling of gavage, the needle is inserted along the corner of the mouth, the throat is found in the middle of the mouth, the mouse is tilted up, and then inserted against the back wall of the throat, if it is correct, the whole root will be very smoothly submerged.

It is poured into the lungs and suffocated to death!

The most important thing is to fix the rat poison well, and the technique is more important. Bitter Chinese medicine or other Western medicine will struggle, so be sure to make sure that the esophagus is in a straight state.

In general teaching, the fingers are taught to hold the head in place and the palms to hold the body in place. The skillful technique is to pinch a little skin behind the ear of the mouse with the thumb of the index finger, hook the tail with the little finger, and let the head be in a state of upturning.

It's hit into the trachea.

1 Whether the gavage reagent is toxic and causes death2 The gastric volume of the mouse is certain, whether the gavage dose is too large3 Whether the gavage operation is proficient, and the mice may also be damaged during the gavage process.

It should be that the gavage needle entered the trachea during gavage, and then injected the drug into the lungs, causing the mice to suffocate to death.

The gavage method for mice is as follows:

First, keep the head and neck of the mouse in a straight line to facilitate the entry of the gavage needle; Then the movements should be gentle, entering through the corners of the mouth, preventing loss of the esophagus. If you do more, you will naturally become proficient.

The specific operation process of gavage in mice is as follows:

1.Prepare the base of the gavage needle to experience the tremor head. Generally, you can buy it from the market, but if you don't have it, you can use a 12-gauge needle, cut off the tip of the needle, and use sandpaper to smooth the tip, or you can use it.

2.However, the tip of the gavage needle bought is treated with tin or a suitable method, and it is impossible to grind off all the sharp incisions with sandpaper, and the possibility of losing the esophagus of mice is greater if the gavage is used with such a needle.

3.Grasp the mouse so that its head, neck, and body are in a straight line. The action of grasping the mouse is very simple, the little finger and ring finger of the left hand grasp the tail of the mouse, and the other three fingers grasp the neck of the mouse, that is, the limb skin can be broken.

Because the mouse is always active, if the feeling of grasping at one time is not very smooth, let go and grasp again, and do not force the next operation.

4.If you grasp the mice, you can gavage, and generally use a 1ml syringe with a substrate to gavage the needle. The gavage needle enters from the corner of the mouth of the round mouse, presses the tongue, against the palate, and gently pushes inward, after entering the esophagus, there will be a sense of prickly hollowness, and after entering the esophagus, the drug can be bolused.

Of course, the gavage holding round needle can also go deeper into the liquid point to prevent the liquid from flowing out of the mouth.

5.The gavage volume is generally the maximum, and the maximum volume of the gavage limb skin loss of each mouse does not exceed.

Consider the possible causes as:

1 Whether the gavage reagent is toxic and causes death.

2. The gastric volume of mice is certain, whether the gavage dose is too large.

3 Whether the gavage operation is proficient or not, it may also cause damage to the mice during the gavage process.

The above is for reference only.

1.Is it that when administering drugs by gavage, we must grasp the rat to make it easy to open its mouth? What are the specific techniques?

The most important thing is to fix the mouse when gavage, if you just make it open its mouth, but not fix it properly, the mouse will not be able to give the gavage smoothly.

The grasping method should be practiced a lot, grasp the head, neck and back of the rat with the left hand, fix it, and make its neck, from the mouth to the stomach, in a straight line, to ensure that the drug enters the stomach, not the trachea. When gavage, you can experience that the needle should be inserted smoothly without a sense of resistance, and the direction of the needle can be slightly adjusted when encountering the bend of the esophagus.

2.What is the method of estimating the length of gavage needle entry?

The gavage needle of the rat is about 6 8 cm long and about 8 cm in diameter.

The depth of needle insertion, in general, is more than two-thirds of the gavage needle, and if the direction is correct, it does not matter if the entire needle is entered.

The tip of the gavage needle is welded with a small round metal ball, which is hollow. The purpose of welding metal balls is to prevent needles from penetrating the trachea or damaging the digestive tract. The metal ball end of the needle is bent at an angle of about 20° to adapt to the physiological curvature of the oral cavity and esophagus.

The curvature of the gavage needle will change during use, so care should be taken to adjust its curvature at all times to the optimal curvature so as not to injure the esophagus).

3.When the gavage needle is placed in the mouth of the mouse, is it easy to be gavaged in the upper part of the mouth or the lower part of the mouth?

There should be no strict distinction, depending on personal experience, I am used to sticking to the base of the tongue of rats to insert needles, while one senior brother is used to pressing the posterior wall of the esophagus into the needle.

Summary. 1.Observe the response of the mice:

After the mice are successfully gaved, there will be reactions such as vomiting, convulsions, and resistance, and if these reactions do not appear, the mice can be judged to have been successfully gavaged. 2.Observe the body weight of the mice:

After successful gavage, the mouse will gain weight, and if the weight does not increase, the mouse can be judged to have failed to gavage. 3.Observe the behavior of the mice:

After the mice were successfully gaved, there would be behaviors such as weakened activity, lack of energy, and weakened resistance, and if these behaviors did not appear, the mice could be judged to have been successfully gaved.

1.Observe the reaction of the mice: after the mice are successfully gaved, there will be vomiting, convulsions, resistance and other reactions, if these reactions do not appear, the mice can be judged to have been successfully gaved.

2.Observe the weight of the mice: after the mice are successfully gaved, the body weight will increase, and if the weight does not increase, the mouse can be judged to have failed to gavage.

3.Observe the behavior of mice: after the mice are successfully gaved, there will be behaviors such as weakened activity, lack of mental cohesion, weakened resistance, etc., if these behaviors do not appear, the success of gavage can be judged in mice.

Can you elaborate on that a little bit more?

The accurate judgment of the success of gavage in mice mainly depends on the reaction of mice, if the mice do not respond, it means that the gavage is successful. When the problem occurs:1

Mice overreact: In this case, the allowable energy is that the speed of gavage is too fast, or the gavage is too much liquid, causing the mice to overreact. The workaround is:

Slowly gavage to control the amount of fluid to be gavaged so that the mice do not overreact. 2.Mice refuse gavage:

In this case, it may be that the mouse is resistant to the liquid given by gavage, or the mouse is resistant to the environment in which the gavage is given. The solution was: changing the gavage environment, changing the gavage liquid, and slowly gavage, so that the mice gradually got used to the process of gavage Mingqiao.

Personal tips: When gavaging small agitated mice, pay attention to controlling the speed and amount of liquid to avoid overreaction of the mice, and also pay attention to the emotions of the mice, so that the mice can gradually get used to the process of gavage, so that the gavage is successful.