To verify the specificity of the enzyme, how to do the experiment?

Teacher preparation]: Consult the materials, design reasonable experimental procedures, analyze the experimental results, and summarize the factors affecting the experimental results and the precautions in the experimental process. Carefully prepare the lesson, design reasonable questions, and guide students to **.

And prepare the specificity of the material apparatus enzyme for the experiment** experiment: one class hour. [Experimental principle] starch can be hydrolyzed into maltose under the catalytic action of salivary amylase.

Under boiling conditions, Filin reagent.

It can oxidize maltose and reduce itself to brick-red cuprous oxide.

Precipitation. Therefore, the Filin reagent can be used to identify the presence of maltose in the solution, and then to see whether salivary amylase can only catalyze the hydrolysis of starch and cannot catalyze other sugars (such as sucrose.

Hydrolysis. Objective requirements] 1. Initially learn the method of doing enzyme specificity experiments. 2 Understand that enzymes are specific.

Material utensils] fresh saliva.

Sterilized absorbent cotton, tweezers, test tubes, small beakers.

Quantity Jane, glass rods, alcohol lamps, matches.

Soluble starch solution with a concentration of 3%, sucrose solution at a concentration of 3%, a newly configured Feilin reagent, and water.

Method step]1 Rinse the mouth with water, and contain a piece of disinfected absorbent cotton in the mouth. Remove the absorbent cotton with tweezers so that the saliva in it collects into a small beaker.

2 Take 3ml of saliva, pour it into another small beaker, and add 30ml of distilled water.

Stir well with a glass rod to make diluted saliva for later use.

3 Take two clean test tubes, numbered, and add the reagents in the following table:

Tube No. 1: 2ml of 3% starch solution plus 2ml of diluted saliva

Tube No. 2: 2ml of 3% sucrose solution plus 2ml of diluted saliva

Shake well and keep warm for 5 min at 37 points

4.Add 2ml of newly configured Filin reagent to the two tubes, shake well, and keep warm at 100 for 3 min

Phenomenon: Tube 1: Brick Red Tube 2: Blue.

Please use the materials and tools provided to design experiments to verify the specificity of the catalytic effects of sucrase and amylase in mammals. It is required to complete the experimental design, supplement the experimental procedure, ** the experimental results, draw conclusions, and ask questions. Experimental materials and utensils:

Suitable concentration of sucrase, salivary amylase, sucrose, starch four solutions, film reagent, test tube, 37 constant temperature water bath, boiling water bath. (1) If "+" represents adding an appropriate amount of solution, "—represents no solution, and A, B, etc. represent the test tube number, please use these symbols to complete the experimental design in the following table, please answer on the answer sheet. Solution Test tube Sucrose solution Starch solution Sucrase solution Salivary amylase solution A + 2) Experimental Procedure:

According to the design in the table above, take the test tube and add the solution. 3) Results**: 4) Conclusion:

5) In the above test, if only the temperature of the 37 thermostatic water bath is adjusted to 20. And if you redo the above experiment all other things being equal, the color in the brick-red test tube will be lighter than at 37, and the reason for this is that

The specificity of the enzyme is demonstrated to catalyze hydrogen peroxide solution with the same enzyme, such as pepsin, while other reactions are catalyzed with pepsin, if the rate of the other reaction does not accelerate significantly.

The activity of the enzyme is related to the temperature and pH of the environment, and some activators and inhibitors can also affect the activity of the enzyme. The main reason is that the influence of temperature and pH is relatively large (without changing the concentration of enzymes and substrates, without adding other reaction reagents).

Temperature, each bucket of enzymes has an optimal reaction temperature. Some may be 35 to 45 degrees of the highest activity, while more than 50 degrees will be denatured and inactivated, and some may be 95 degrees of dispersion The highest activity. Therefore, keeping the enzyme close to its optimal reaction temperature will ensure the activity of the enzyme.

pH, environmental pH, too acid, too alkali will affect the conformation of enzyme proteins, destroy the structure of proteins, and cause enzyme denaturation and inactivation. At pH optimum, the dissociation state of the group in question on the active center of the enzyme molecule is most suitable for binding to the substrate. The activity of enzymes is the highest.

Therefore, the enzyme should be stored in the buffer solution at the optimal pH.

Middle. The other is to change the concentration of the enzyme or substrate, and the addition of activators or inhibitors will also change the activity and reaction time of the enzyme.

Summary. Starch is catalyzed by salivary amylase and can be hydrolyzed into maltose. Under boiling conditions, the Feilin reagent can oxidize the maltose and reduce itself to a brick-red cuprous oxide precipitate.

Therefore, the Filin reagent can be used to identify the presence of maltose in the solution, and then it can be seen whether salivary amylase can only catalyze the hydrolysis of starch and cannot catalyze the hydrolysis of other sugars (such as sucrose).

Enzyme specificity experiment report results analysis, why the results are produced.

The experimental principle is as follows.

Under the catalysis of salivary amylase, starch can be hydrolyzed into maltose spreptocia. Under the boiling of the strip call collapse piece, the Feilin reagent can oxidize the wheat bud candy and reduce itself to a brick-red cuprous oxide precipitate. Therefore, the Filin reagent can be used to identify the presence of maltose in the solution, and then it can be seen whether salivary amylase can only catalyze the hydrolysis of starch and cannot catalyze the hydrolysis of other sugars (such as sucrose).

Analysis of experimental results.

What is the role of each group of distilled water? Boil the enzyme for 10 minutes and repeat the above experiments.

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Analysis: 1. Experiment on the high efficiency of the enzyme: compare the catalytic efficiency of catalase Jingkuan and Fe3 to verify the high efficiency of the enzyme.

2. Experiment on the specificity of filial piety enzyme: explore the effect of amylase on starch and sucrose to verify the specificity of enzyme.

Take two test tubes A and B, add 1 ml of sucrose solution to the A test tube, add 1 ml of starch solution to the B test tube, add the same amount of salivary amylase 1 ml respectively, add the same amount of film reagent to the two test tubes, heat in a water bath, the experimental results are that the A test tube has no color, and the B test tube has a brick-red Shenshan Mindian.

The results indicated that starch was decomposed into reducing sugars by salivary amylase, while sucrose or silver branches were decomposed.

Prove that enzymes are specific!

Demonstrate the specificity of the enzyme: catalytic hydrocarinase peroxide can only catalyze the decomposition of hydrogen peroxide, not other chemical reactions. Cellular metabolism can be carried out in an orderly manner, which is inseparable from the specificity of enzymes.

Take two test tubes, denoted as A and B, add starch to A and add sucrose to B. Amylase was added to both tubes and a constant temperature water bath was placed for 37 times. Add the Filin reagent to both tubes and observe the color change.

Results: Brick-red precipitate appeared in tube A, but there was no change in tube B. Because amylase can only break down starch to produce reducing sugars.

The enzyme has strict selectivity for the substrate it acts on. An enzyme can only act on one substance, or a class of substances with similar molecular structures, to promote it to carry out a certain chemical reaction and produce certain reaction products, and this selective effect is called the specificity of the enzyme.

Usually enzymes can only catalyze a chemical reaction or a similar reaction, and enzymes with different closed suits have different degrees of specificity, and the specificity of enzymes can be divided into three types: absolute specificity, relative specificity, and three-dimensional specificity; It can also be divided into: structural specificity and stereoheterogeneous specificity.

Hello, enzymes are catalysts that are capable of accelerating the rate of chemical reactions, but at the same time they are specific. The specificity of enzymes is Hayano which means that they can only catalyze specific reactions and not others. This specificity is determined by the structure and center of activity of the enzyme.

Therefore, verifying the specificity of an enzyme requires the use of substrates that react specifically with that enzyme.

Sucrase is an enzyme that catalyzes the breakdown of sucrose into glucose and fructose. Although sucrase is a common enzyme, it is not suitable for verifying enzyme specificity. This is because sucrase has a wide substrate specificity, which can catalyze not only the decomposition of sucrose, but also the decomposition of many other sugars, such as maltose, lactose, etc.

Therefore, it is inaccurate to verify the specificity of the enzyme using sucrase.

In order to verify the specificity of an enzyme, a specific substrate is required to detect the activity of the enzyme. For example, if you want to verify the specificity of lipase, you can use triacylglycerol as a substrate, because lipase can only catalyze the hydrolysis of triacylglycerol. If the specificity of amylase is to be verified, starch can be used as a substrate because amylase can only catalyze the hydrolysis reaction of starch.

In summary, validating the specificity of an enzyme requires the use of substrates that react specifically with that enzyme, rather than enzymes with broad substrate specificity.

Validating the specificity of the enzyme means that the enzyme activity is only activated by a specific substrate and is not affected by other substances. Sucrose is a specific enzyme that can only catalyze the decomposition of sucrose into fructose and glucose, but not other sugars. Therefore, it is unscientific to use sucrase to test the specificity of the enzyme because it does not fully reflect the specificity of the enzyme.

To verify the specificity of the enzyme, different carbohydrate finger substrates should be used, and if the same substance can only catalyze a specific substrate and is not affected by other substances, then it has good specificity. In addition, other chemical reagents, such as ammonium sulfate or sodium sulfate, can be used to test enzyme resistance to determine enzyme specificity.

Because sucrose can be hydrolyzed by amylase or not, iodine solution will not turn blue. No, the temperature will affect the rate of hydrogen peroxide, with starch, sucrose and amylase. Because sucrose can be hydrolyzed by Yodoyuna powder or not, the iodine solution will not turn blue.

No, the temperature affects the rate of decomposition of hydrogen peroxide, with starch, sucrose and amylase.

Summary. A positive reaction to the specificity of the enzyme in question means that in the experiment, the enzyme can only catalyze a specific substrate and not other substrates, resulting in a specific product, thus indicating that the enzyme is specific. Workaround and Procedure Steps:

1.First, prepare a specific enzyme and mix it with different substrates to detect the specificity of the enzyme. 2.

Second, the mixture is added to the reaction tube and the reaction is performed at the appropriate temperature and pH conditions to detect the specificity of the enzyme. 3.Finally, observe the reaction products in the reaction tube, if only a specific substrate produces a specific product, it indicates that the enzyme is specific.

Related knowledge: Enzyme specificity means that enzymes can only catalyze specific substrates, but not other substrates, resulting in specific products. Enzyme specificity is an important property of enzymes, which can help us understand the structure and function of enzymes and can help us make better use of enzymes.

A positive reaction to the specificity of the enzyme in question means that in the experiment, the enzyme can only catalyze a specific substrate and not other substrates, resulting in a specific product, thus indicating that the enzyme is specific. Workaround and practice steps:1

First, prepare a specific enzyme and mix it with different substrates to detect the specificity of the enzyme. 2.Second, the mixture is added to the reaction tube and the anti-stool response is performed at the appropriate temperature and pH conditions to detect the specificity of the enzyme.

The specificity of enzymes means that enzymes can only catalyze specific substrates per mu, but cannot catalyze other substrates, so as to produce specific products. Enzyme specificity is an important property of enzymes, which can help us understand the structure and function of enzymes, and can help us make better use of enzymes.

Fellow, I really didn't understand, I can be more specific.

A positive reaction to the specificity of the enzyme solution to the problem means that in the experiment, the enzyme can specifically catalyze a specific substrate without catalyzing other substrates, resulting in a specific product, thus exhibiting the specificity of the enzyme. Expand on some related content: The specificity of enzymes is an experimental method used to detect the specificity of enzymes, which can be used to detect whether enzymes have specific substrate specificity and whether enzymes have specific product specificity.

It can be used to detect enzyme activity, as well as enzyme structure and function. In addition, it can be used to detect the stability of enzymes, as well as changes in the structure and function of enzymes. Enzyme specificity experiments can be used to study the properties of enzymes, as well as the structure and function of enzymes.

It can be used to study the activity of enzymes, as well as changes in the structure and function of enzymes. In addition, it can be used to study the stability of enzymes, as well as changes in the structure and function of enzymes. In addition, it can also be used to study changes in the structure and function of enzymes, as well as changes in the stability and activity of enzymes.

In summary, the specificity of the enzyme experimental positive reaction refers to the fact that in the experiment, the enzyme can specifically catalyze a specific substrate without catalyzing other substrates, thus producing a specific product, thus showing the specificity of the enzyme. It can be used to study the properties of enzymes, as well as the structure and function of enzymes, as well as changes in the stability and activity of enzymes.