Who knows the formula of LB medium? Where can information on biotechnology be found?

The landlord can go to the biological gang to learn more about it.,The technical documentation there is quite comprehensive.,And there's a **tutorial or something.,I'm going there to find the information I want.。 I've helped you find a recipe for LB medium there. LB medium, liquid medium, according to the "Molecular Cloning Experimental Guidelines" (J

Sambrook Russell) to prepare the medium, which should be added in 950 ml of deionized water: tryptone 10 g yeast extract 5 gnacl 10 g shake the container until the solute is dissolved. Adjust pH with 5mol LNAOH to 1L. with deionized water

Steam sterilization medium under high pressure at 15psi is the name of a culture medium, which is generally used to pre-cultivate strains in biochemical molecular experiments, so that the strains can be multiplied and expanded to meet the requirements of use. Cultured strains are generally engineered strains that cannot survive and expand independently in the external environment. By cultivating engineered bacteria, we can express a large number of exogenous proteins, and we can also obtain plasmids with exogenous genes, and the effective amplification of engineered bacteria is the basis of biochemical molecular experiments.

The formula of LB medium is as follows: tryptone (tryptone) 10g L yeast extract (Yeast extract) 5g L sodium chloride (NaCl) 10g L In addition, adjust the pH of this medium with NaOH according to the empirical value to make it suitable for the growth of the most widely used prokaryotic expression strains at present) Solid medium LB solid medium 1L is the same as liquid, add 15g agar powder, be sure to add antibiotics before the temperature drops, and pour the plate. LB solid medium inverted plate 1

Preparation: 100 ml LB medium with agar powder 2Addition of antibiotics:

After autoclaving, put the melted LB solid medium in a water bath with 55, and add antibiotics when the temperature of the medium drops to 55 (touchable by hand) to avoid the antibiotics from being invalid due to too high temperature, and shake well. 3.Inverted board:

Generally 10ml pour 1 plate. After pouring the medium into the Petri dish, open the lid and shine under UV light for 10-15 min. 4.

Look it up for yourself. Hope it helps.

<>lb medium.

The recipe is as follows:

tryptone 10 g l;

Yeast extract.

yeast extract) 5g/l；

Sodium chloride. nacl) 10g/l

In addition, Xiaoxiang adjusted the pH of the medium with NaOH according to the empirical value, so that it reached the cavity to the pH suitable for the most widely used prokaryotic expression strains before eye contact round beating.

LB medium is the name of a culture medium, which is generally used to pre-culture strains in biochemical molecular experiments, so that the strains are multiplied and expanded to meet the requirements of use. It can be divided into liquid medium and solid medium.

The formulation of <>lb medium is as follows:

tryptone 10 g l;

Yeast Extract 5g L;

Sodium chloride (NaCl) 10 g L

In addition, according to the empirical value, Xiaoxiang adjusted the pH of the medium with NaOH to make it suitable for the growth of the most widely used prokaryotic expression strains at present).

LB medium is the name of a culture medium, which is generally used to pre-culture strains in biochemical molecular experiments, so that the strains can be multiplied and expanded to meet the requirements of use. It can be divided into liquid medium, cavity draft and solid medium.

The formulation of solid LB medium is tryptone (tryptone) 10 g l yeast extract (yeastextract) 5 g l sodium chloride (NaCl) 10 g l.

The formulation of solid LB medium is tryptone (tryptone) 10 g l yeast extract (yeastextract) 5 g l sodium chloride (NaCl) 10 g l. In addition, adjust the pH of the medium with NaOH according to the empirical value, so that it reaches this pH, which is suitable for the growth of the most widely used prokaryotic expression strains) Solid medium LB solid medium 1L is the same as liquid, add 15g of 20g agar powder, be sure to add antibiotics before the temperature drops, and pour the plate.

Precautions for solid LB medium:

1. Pay attention to aseptic operation to avoid microbial contamination.

2. According to the growth characteristics of the strain, the pH value can be adjusted appropriately.

3. The medium is only used in the field of scientific research, and should not be used for clinical diagnosis or other purposes.

4. Pay attention to dust when weighing, and wear a mask to avoid respiratory system discomfort.

5. Screw the cap immediately after the use of dry powder medium to avoid moisture absorption and agglomeration. The shelf life of unopened pure sales and packaging products is three years, and there are certain differences in shelf life according to different storage conditions after opening. <>

LB (Luria-Bertani) medium is a commonly used bacterial culture medium, and its main components include three main components: beef extract, yeast powder, and the preparation of the medium is relatively simple, but there are some key steps that need to be paid attention to to ensure the quality and stability of the medium. The preparation methods and precautions of LB medium are described in detail below.

1. Formulation of LB medium.

The formulation of <>lb medium is as follows:

Beef extract: 10 g l

Yeast powder: 5 g l

NaCl: 10 g L

Water: 1000 ml

2. Preparation steps of LB medium.

1.Weigh the desired beef extract, yeast powder and NaCl, add to about 500 ml of deionized water, and stir vigorously to dissolve thoroughly.

2.Add enough deionized water to bring the total round volume to 1000 ml.

3.The mixture is poured into the burnt flask and autoclaved with an automatic pressure sterilizer. If an automatic sterilizer is not available, sterilization can also be performed with a pressure cooker.

4.After sterilization, the flask is placed at room temperature, and the medium is cooled to room temperature before it can be used or stored.

3. Precautions for LB medium.

1.Selection of materials: The quality and stability of LB medium is related to the raw materials used. Therefore, high-quality beef extracts, yeast powder, and NaCl need to be selected to ensure the quality and stability of the medium.

2.Stirring time: During the preparation process, the mixture needs to be stirred well to ensure that the raw materials are fully dissolved. Stirring is usually required for 10-20 minutes until the mixture becomes homogeneous.

During the sterilization process, it is necessary to pay attention to the temperature and time of sterilization. Usually use an autoclave and sterilize at 121 °C for 20-30 min. If using a pressure cooker for sterilization, Kamiga Jane needs to be sterilized at 100°C for 30-60 min.

4.Cooling time: After sterilization, the burned flask needs to be placed at room temperature to cool to room temperature before use or storage. If used or stored immediately after sterilization, it may affect the quality and stability of the medium.

5.Storage conditions: LB medium can be stored at 4 °C for 1-2 months or at -20 °C for more than 6 months. During storage, care needs to be taken to avoid light and shaking to avoid affecting the quality and stability of the medium.

In conclusion, LB medium trousers are a commonly used bacterial medium that is relatively simple to prepare, but there are some key steps to be aware of. In the preparation process, it is necessary to select high-quality raw materials, stir the mixture thoroughly, sterilize it correctly, and cool it to room temperature before use or storage to ensure the quality and stability of the medium.

<>lb medium.

For each liter of medium to be prepared, 950 ml of deionized water should be added to the lead of the survey

Tryptone 10g

Yeast extract 5g

nacl 5g

Shake the container until solute dissolves. Adjust the pH with 5mol lnaoh to 1LSteam sterilize at 15 psi autoclave for 20 min

After the LB medium is out of the pot, when the temperature drops to the point that it is not too hot, add ampicin for filtration and sterilization (syringe and needle filter for filtration and sterilization), and shake the medium well.

If the LB medium with agar powder must be added before solidification When I did the experiment, it was about 55 degrees, and I could pick it up by hand.

1. Preparation: 100mlLB medium.

Add agar powder.

2. Add antibiotics: After autoclaving, put the melted LB solid medium in a water bath with 55, and add antibiotics when the temperature of the medium drops to 55 (touchable by hand), so as to avoid the ineffectiveness of antibiotics caused by too high temperature, and shake well.

3. Inverted board: generally 10ml pours 1 board. After pouring the medium into the Petri dish, open the lid and shine under UV light for 10-15 min.

4. Preservation: Seal the edge with sealing glue, and put it upside down in 4 to save, and use it within one month.

The formulation of <>lb medium is as follows:

tryptone 10 g l;

Yeast extract 5g l;

Sodium chloride (NaCl) 10 g L

In addition, according to the empirical value, adjust the pH of the medium with NaOH to make it reach, LB medium is the name of a medium of lead shade cherry, and the medium is generally used in biochemical molecular experiments to pre-cultivate strains, so that the strains can be doubled and expanded, and can be divided into liquid medium and solid medium.

LB mediumA commonly used medium for culturing genetically engineered receptor bacteria (Escherichia coli) in recent years. Both belong to semi-synthetic media. The main components of the medium of meat paste peptone are beef paste, peptone and NaCl.

The main components of LB medium are tryptone, yeast extract, and NaCl. Gao's Synthetic Medium No. 1 is a synthetic medium used for the cultivation of actinomycetes. Soluble starch in the medium serves as a carbon source and energy source.

The above inner locust cluster capacity reference: Encyclopedia-LB medium.