What is the difference between high and low sugar in DMEM medium

1. Different uses: low-sugar DMEM is used to raise stem cells to prevent differentiation, and high-sugar DMEM can raise most mammals.

of cells. 2. Different applicability: high-glucose DMEM is suitable for cultivating cells with vigorous metabolism, while low-glucose is suitable for cultivating cells with general metabolic level. Metabolically active cells, such as ES, low glucose medium.

Insufficient carbon sources are not available.

3. Different properties: low-sugar yeast is suitable for survival in sugar concentration below 7%, while high-sugar yeast is suitable for survival in sugar concentration above 7%.

Blood glucose levels and manifestations are different.

The difference between high blood sugar and low blood sugar is more obvious, first from the check of blood sugar, one is blood sugar, which is the diagnostic standard for hypoglycemia, and the other is the blood sugar of diabetic patients taking drugs, which is also hypoglycemia, but high blood sugar is fasting blood sugar above 7mmol L, or postprandial blood sugar must be above, or blood sugar may appear at any time, in short, low blood sugar is low when blood sugar is measured, high blood sugar is high when blood sugar is measured, and its clinical manifestations are also different, such as hypoglycemia, There may be mood swings, headaches, paleness, sweating, dizziness, tremors, fatigue, hunger, etc., all of which may occur during hypoglycemia, and may also have abnormal behavior, confusion, and coma in severe cases. However, in hyperglycemia, there are also its manifestations, such as high blood sugar, there may be obvious symptoms of "three more and one less", such as some patients are not typical, just feel tired, sleepy, dry mouth, polyuria, thirst and other conditions. In short, the difference between hypoglycemia and hyperglycemia is quite obvious, first from the numbers, check one is low blood sugar, the other is high blood sugar, and then the clinical manifestations.

The high-glucose DMEM medium contains 4500 mg L of glucose, which is suitable for tumor cells that grow faster and have poor attachment. The low-sugar type is 1000 mg l.

When doing monoclonal antibody cell fusion, low sugar is generally used, and high sugar makes the cell grow too fast, which is not conducive to the chromosomal stability of the fusion cell. Cells can be cultured stably for more than 10 passages and can be cultured with high-glucose DMEM.

DMEM medium is a medium containing various amino acids and glucose, which is developed on the basis of MEM medium. Compared with MEM, the dosage of various ingredients is increased, and it is divided into high sugar type (less than 4500 mg L) and low sugar type (less than 1000 mg L). The high glucose type is conducive to the growth of cells in one location, and is suitable for tumor cells that grow faster and have difficulty attaching to them.

Difference: Ordinary DMEM refers to low DMEM, which has an internal glucose content of 1100 milligrams. High sugar DMEM, the content of which is 4500 mg liters.

The former is used to raise stem cells to prevent differentiation, and the latter can feed most mammalian cells.

DMEM is a medium containing various amino acids and glucose, which is developed on the basis of MEM medium.

DMEM medium has a difference between high sugar and low sugar, and high sugar DMEM medium contains 4500mg L of glucose, which is suitable for tumor cells with fast growth and poor attachment. The low-sugar type is 1000 mg l.

All the inverted macros were searched into a container, and the residual medium in the bag was washed off with double distilled water and incorporated into the container. Add 950 ml of imitation water for injection (water temperature 20-30 ml) and stir slightly to dissolve.

2.Add grams of sodium bicarbonate.

Dissolve with slight stirring, 3with 1mol l sodium hydroxide.

solution or 1mol l hydrochloric acid solution to adjust pH to the desired value, add double distilled water to 1 liter.

4.Sterilize by positive pressure filtration with a filter membrane.

5.The solution should be stored at 2-8 degrees in the dark of the place.