Step 10 of the method of fermentation and activation of the culture

The main components of yeast.

Protein

Carbohydrate

Fat fat

Nucleic acid

Minerals

vb1 vitamin1

165 ppm

vb2 vitamin2

100 ppm

Niacin niacin

585 ppm

Pantothenic acid

100 ppm

Folic acid

13 ppm

Appendix Requirements for the life of yeast in terms of external conditions.

Nutrition: Yeast, like other living organisms, needs similar nutrients, like bacteria, it has a set of intracellular and extracellular enzyme systems to break down macromolecular substances into small molecules that are easily used by cellular metabolism; Moisture: Like bacteria, yeasts must have water to survive, but yeasts require less water than bacteria, and some yeasts can grow in environments with very little water, such as honey and jam, which suggests that they are quite tolerant to osmolality.

Acidity: Yeast can grow in the pH range of 3, optimum pH.

Temperature: Yeast cells generally cannot grow at temperatures below the freezing point of water or above 47, and the optimal growth temperature is generally between 20 and 30. Yeast sleeps at low temperature 0.

Oxygen: Yeast grows in both aerobic and anaerobic environments, i.e. yeast is facultative anaerobe, and in the absence of oxygen, yeast breaks down sugar into alcohol and water. In the presence of aerobic, it breaks down sugars into carbon dioxide and water, and yeast grows faster in the presence of aerobic.

Appendix Yeast activity test.

After the yeast is added to 5 times the volume of sugar water (sugar water concentration 10%, temperature 35) for a few minutes, the yeast sugar water should produce foam, and the foam rise volume is about equal to the volume of sugar water.

Protein

Carbohydrate

Fat fat

Nucleic acid

Minerals

vb1 vitamin1

165 ppm

vb2 vitamin2

100 ppm

Niacin niacin

585 ppm

Pantothenic acid

100 ppm

Folic acid

13 ppm

1. First prepare sodium hydrochloride, yeast and sodium chloride solution.

and a syringe tube.

2. Mix the yeast and sodium hydrochloride and mix well with the stirring rod.

3. There will be flocculent viscous liquid during the stirring process, so it must be stirred and mixed evenly to make it completely integrated.

4. Use a syringe.

Absorb a portion of the mixture of yeast and sodium hydrochloride.

5. Use a syringe to drop the solution vertically into the chlorine and sodium solution (note that it must be dropped vertically).

6. Finally, immobilize the yeast.

It is formed, and other experimental operations can be carried out.

Strain activation is to put the preserved strains into a suitable medium for culture, and gradually expand the culture in order to obtain a pure and strong culture, that is, to obtain a vigorous and sufficient number of inoculated cultures. The fermentation of the strain generally requires 2-3 generations of rejuvenation process, because the conditions at the time of preservation are often different from the conditions at the time of culture, so it is necessary to activate the strain and gradually adapt to the culture environment.

In order to understand the activation of cultures, it is necessary to understand the ways and methods of culture preservation. At present, the commonly used methods for the preservation of strains at home and abroad include: regular transplantation method, liquid paraffin method, sand tube method, vacuum freeze-drying method, 80 freezer freezing method, and liquid nitrogen ultra-low temperature freezing method.

The way of activation is also different for different preservation methods:

1. The recovery of strains by regular transplantation is relatively simple, and direct transfer is sufficient;

2. When the strains preserved by the liquid paraffin method are recovered, a small amount of bacteria should be selected and transferred to a suitable fresh medium, and after growth and reproduction, they should be transferred again;

3. When the sand and soil tube method preserves the bacteria, open the sand tube under aseptic conditions, take part of the sand and soil particles on the appropriate inclined medium, and transfer them again after the bacteria grow. or take the sand and soil particles in a suitable liquid medium, proliferate and culture, and then transfer to the inclined plane;

4. Wipe the upper part of the ampoule with 70% alcohol cotton during the recovery of the strain preserved by vacuum freeze-drying method, heat the top of the ampoule tube, dip it in cold water with a sterile cotton swab, wipe it around the top, crack on the top, knock off the top of the cracked ampoule tube with a knife or tweezers, dissolve the bacterial block with sterile water or culture medium, and use a sterile straw to move it into the fresh medium for appropriate temperature culture;

5 -80 When the freezer preservation method is used to preserve the resuscitation of the culture, take out the ampoule or plastic cryopreservation tube from the refrigerator and immediately place it in a 38 -40 water bath for rapid recovery and shake it appropriately and quickly. It takes about 50-100 seconds until all the internal ice is dissolved. Open the ampoule or plastic cryovial and transfer the contents to the appropriate medium for incubation.

6 The recovery of the strains preserved by the liquid nitrogen ultra-low temperature freezing method is similar to the recovery of the strains preserved by the -80 freezer freezing method, and the ampoule or plastic cryopreservation tube should be removed from the liquid nitrogen tank and should be immediately placed in a 38 -40 water bath for rapid recovery and shaken appropriately. It usually takes about 50-100 seconds until all the internal ice is dissolved. Open the ampoule or plastic cryovial and transfer the contents to the appropriate medium for incubation.

To summarize the following steps, strain activation requires the following steps:

The first is to configure a medium suitable for the growth of strains.

Second, restore the culture from its storage state to room temperature, such as thawing the frozen culture.

Thirdly, the preserved strains will be inoculated into the culture medium for culture, and this step is called strain rejuvenation.

Step 4: Pick colonies with a strong medium, inoculate some of them into a new medium, and repeat this step 2-3 times to obtain well-grown colonies.

After these four steps, the purpose of activating the culture from the preservation state is achieved.

Hope it helps!

Some students have already said that it is very professional, so I will tell you something simple and practical. I don't know for what purpose you're going to do it. If you are using a cryovial to activate the strain, take out the 2 beads and place them on the appropriate solid medium, close the lid and let the beads roll on the plate, and the appropriate conditions can be cultivated.

Grow a colony and you're good to go. If you are a lyophilized powder strain, prepare a suitable liquid medium, a little less powder inoculation can be, according to the general liquid culture time of this strain, about 36-48h, you can also observe that the liquid is turbid, there is a precipitation at the bottom of the test tube (the precipitator may also be the original dead bacteria), take 100 microliters of liquid and apply it to the solid medium.

In general, the resuscitated strain needs to be repeated several times.

Depending on how the strain is preserved, different preservation methods and activation methods are different. You can enter the ** of the China Industrial Microbial Culture Collection Center to see, there are specific activation methods.

Resuscitation of preserved cultures is required when they are reused. Strain activation is similar to strain recovery, the difference is that strain activation is a step on the basis of strain recovery, that is, to make the growth vitality of strains stronger, usually in a suitable medium and suitable culture conditions through continuous subculture to enhance strain viability.

The direct scribing of the liquid glycerin tube 37 culture (it can grow in about a day) is fine, and the solid one is not very clear

Hello, I am happy to serve you and give you the following answers: The treatment methods after the activation of the strain silver teasing mainly include the following steps:1

Put the activated strains into a container, add an appropriate amount of medium, and stir well; 2.Put the stirred strains into a petri dish, add an appropriate amount of medium, put it in an incubator, and control the temperature and humidity; 3.Put the Yinxian Petri dish into the incubator and control the incubation time, generally 24-48 hours; 4.

Put the cultured strains in the refrigerator and store them; 5.Place the culture in the refrigerator and check the activity of the culture at regular intervals, if the activity is not good, it can be reactivated. Personal Tips:

The treatment after the activation of the strain is very important, and it is necessary to pay attention to the control of the incubation time, the temperature and humidity of the control of the strain, so as to ensure the activity of the strain. In addition, the activity of the culture should be checked regularly, and if the activity is not good, it can be reactivated.

Hello, I am happy to serve you and give you the following answers: The treatment methods after the activation of the strain mainly have the following steps:1

Put the activated strains into a container, add an appropriate amount of medium, and stir well; 2.Put the stirred strains into a petri dish, add an appropriate amount of medium, put it in an incubator, and control the temperature and humidity; 3.Put the Petri dish into the incubator and control the incubation time, generally 24-48 hours; 4.

Put the cultured strains in the refrigerator and store them; 5.Put the preserved strains into the culture change dish, add an appropriate amount of medium, put it in the incubator, control the temperature and humidity, and the culture time is generally 24-48 hours; 6.Place the cultured culture in the refrigerator and store it for next use.

The above is the treatment method after the activation of the strain, in the treatment process, pay attention to control the culture time, temperature and humidity to ensure the activity of the strain.