EM culture medium and fermentation method 10

Medium for embarrassment.

Lactic acid bacteria can be cultured.

5g, distilled water.

1000ml, preparation method: mix according to the above ingredients, correct pH after dissolving, 121 autoclave for 15min

2 Nutrient agar medium.

Ingredients: peptone 10g, beef paste 3g, sodium chloride 5g, agar 17g, distilled water 1000ml, preparation method: dissolve the components except agar in distilled water, correct pH, add agar, divide in the flask, 121, 15min autoclave for later use.

3 MRS medium.

Ingredients: Peptone 10g, Beef Paste 10g, Yeast Powder 5g, K2HPO4 2g, Diammonium Citrate 2g, Sodium Acetate.

5 g, glucose.

20g, Tween 80

1ml,mgso4 .7H2O, MnSO4 4H2O, (agar 15 20 g), distilled water 1000 ml

Method: Add the above ingredients to distilled water, heat to completely dissolve, adjust pH, divide in triangular bottles, 121, sterilize for 15min

4 skim milk medium.

Ingredients: Milk, distilled water.

Method: Heat and boil an appropriate amount of milk for 20 30min, cool overnight, and the fat can float. Skim milk is obtained by removing the upper layer of milk fat.

The skim milk is put in a test tube and a triangular bottle, sealed and placed in a sterilization pot and steam sterilized under 108 conditions for 10 15min, that is, the skim milk medium is obtained.

The fermentation method is still very simple, but to prepare the ingredients, you need to prepare the starter culture, a small amount of brown sugar, cornmeal or wheat bran.

Commonly used cattle and sheep feed production methods:

Silage: After the feed silage, a large number of vitamins, minerals, proteins can be preserved, with the characteristics of soft, sweet, sour, ripe and fragrant, if urea, ammonia, protein and other substances are added during the preparation, the silage quality will be better. However, silage has high acidity and laxative effect, and it needs to have a period of adaptation when starting to feed, and it should be matched with coarse material and concentrate, and cannot be used alone.

Feed pregnant ewes as little as possible, and stop feeding 15 days before farrowing. If the silage is too acidic, it can be neutralized with 5%-10% lime milk and fed.

Hay feed: The palatability of hay feed is poor, and some forage grasses are easy to separate stems and leaves after drying, sheep are often picky eaters. Therefore, when feeding sheep with green hay, it needs to be cut into centimeter pieces, and it can be shorter when feeding old and weak sheep and lambs.

Straw feed: straw is characterized by long, coarse, hard, not convenient for sheep to eat, and the digestibility is not high, in addition to cutting short feeding, can be used soaking, ammoniation, alkalization, fermentation for modulation.

Fermentation method: The commonly used method is EM bacteria fermentation. Take 1 bag of Yifuyuan straw starter agent, add 1 kg of brown sugar, 10 kg of water, mix it well, put it into a sealed container, ferment for 5 days, and then dilute it into 600 kg of water with water, spray it on 2000 kg of crushed straw, fill it in the fermentation tank, seal it for half a month, and open the cellar for use.

What are the benefits of using straw starter to ferment dried corn stalks to feed cattle and sheep:

1. Shorten the maturity time of straw feed silage and yellow silage, and improve the quality of feed storage.

2. Improve the palatability of feed, the feed texture is soft, mellow, sour, fruity, the feed intake is increased by 20%-40%, and the feed intake rate is increased by more than 20%. Animals have developed a hobby for microbial protein amino acids, lactic acid bacteria, and yeasts just like the ingredients in amino acid oral liquid, yogurt, and beer that humans drink, and love to eat.

3. Significantly increase the nutrient composition of the feed, increase the softness and swelling of the straw, so that the rumen microorganisms can directly contact with the cellulose, and improve the digestibility of the straw. Cattle and sheep fed microsilage can improve the lipolytic enzyme activity of cattle and sheep rumen microflora cellulase, which can promote the generation of volatile fatty acids, which can provide a carbon frame for the synthesis of microbial bacterial proteins, and propionic acid is an important grape precursor for ruminant livestock.

4. Improve the utilization rate of feed digestion and absorption, improve production performance, contain a variety of beneficial microorganisms, establish a microecological balance in the animal intestine, and completely absorb and utilize the nutrients in the feed by animals, which can make the utilization rate of protein, energy and minerals reach 95%, and greatly improve the absorption and utilization rate of crude fiber feed (straw, chaff meal, bran), so it can reduce feed costs, and long-term use can save 10-25% of feed.

5. Improve immunity, prevent and improve intestinal diseases, establish intestinal microecological balance, inhibit the reproduction of harmful bacteria, and increase the reproduction of beneficial microorganisms.

What kind of crop straw do you want to ferment? Can you tell us about that? Because different straw fermentation methods may not be the same.

The fermentation method is as follows:

1. Ratio: The proportion of EM bacteria, 10g (EM bacteria): 1kg (brown sugar): 10kg (sterile water).

2. Cultivation: Brown sugar is heated and dissolved with water, EM strains are added at 30-40 degrees water temperature, the container is covered and sealed, the temperature is controlled at 30-35 degrees and fermented for 5-7 days, and the sour fragrance can be smelled.

Summary. Dear, I'm glad to answer for you. The best method of EM bacteria fermentation is:

The fermentation method is as follows: 1. Ratio: the proportion of EM bacteria, 10g (EM strains):

1kg (brown sugar): 10kg (sterile water). 2. Cultivation:

Brown sugar is heated and dissolved with water, EM strains are added at 30-40 degrees water temperature, the container is covered and sealed, the temperature is controlled at 30-35 degrees and fermented for 5-7 days, and the sour fragrance can be smelled. <>

The best method for fermentation of EM bacteria.

Dear, orange smile is happy to answer for you. The best method of EM bacteria fermentation is as follows:

1. Ratio: The ratio of EM bacteria to brother tremor, 10g (EM bacteria): 1kg (brown sugar):

10kg (sterile water). 2. Cultivation: Brown sugar is heated and dissolved with water, EM strains are added at 30-40 degrees water temperature, the container is covered and sealed, and the temperature is controlled at 30-35 degrees for fermentation for 5-7 days.

Pro-EM bacteria are a kind of mixed bacteria, which generally include photosynthetic bacteria, yeasts, lactic acid bacteria and other beneficial bacteria. EM bacteria are fermented to form EM bacteria stock solution. Note:

The most difficult thing to master in the cultivation process is the temperature mold grouping degree, and the temperature is controlled at a maximum of 40 degrees. After more than 40 degrees, the number of bacteria in EM bacteria will be reduced by Dantan orange. During the fermentation process, the temperature is best controlled between 30-35 degrees.

It's better to have a lower temperature than a little higher! If the temperature is low, the fermentation time of EM bacteria can be extended without causing loss of culture. We hope I can help you<>

The method of making em bacteria is as follows:

Method 1: Prepare 2kg of boiling water, add 1kg of brown sugar, stir and dissolve, then add 8kg of purified water, and add 10g of bacteria at a temperature of no higher than 40. Put the whole mushroom water into a sealed plastic container to ferment, and after 7-12 days, you can open the bottle mouth, smell the sweet and sour smell, and the production is successful.

Method 2: Pour cold boiled water and bacterial mother liquor into a plastic bucket, then add brown sugar, urea, and potassium dihydrogen phosphate to the plastic bucket, stir evenly and seal the fermentation. When the fermentation temperature is 35 °C, stir with the lid every 2 days, generally about 7 days in summer and about 15 days in winter.

When you open the container, you can smell the sweet and sour smell, and the fermentation is successful, and it can be used as a bacterial liquid.

The standard of EM bacterial solution: the color is brownish-red, the odor: sweet and sour, pH: 3 4.

The advantage of the first method is that it is simple, convenient and easy to operate, and the disadvantage is that the fermentation is not as sufficient as the second method; The second method is more effective in making the stock solution. If you want to make your own EM liquid at home, it is recommended to use the second method.

EM bacteria, many people know that it is a kind of microbial fungus agent, made of biological fertilizer, widely used in agricultural planting, with insecticide, disease, increase yield, improve soil, no pollution advantages, so it is also known as "universal bacteria".

1. Sterilize plastic buckets, measuring cylinders and other utensils for later use. Usually, after washing filial piety, it is dried in the sun, which can wipe off the moisture on the surface and also play a role in sterilization. If it's rainy, blanch it with boiling water and let it dry.

2. Put 2 kg of brown sugar into the sterilized plastic bucket of He Shenyu, boil 18 kg of water, pour it into the bucket, then add 1 kg of plastic bucket and close the lid. When the water temperature drops to about 35 degrees, add a bottle of EM culture (10 grams) and finally close the lid tightly. In the hot season, it can be placed in a cool place, and the temperature needs to be heated when the temperature is below 20 degrees.

The best thing to do is to choose a sunny day and let it dry in the sun for two to three days to speed up fermentation. 3. When the temperature is 25-35, foam will appear on the water surface on the third day of fermentation. On the eighth day, the foam will slowly disappear, and a white suspended matter will float on the surface of the water.

At this point, although the fermentation is not fully completed, it can be used for production. By the 13th day or so, the suspended solids sink to the bottom of the barrel and fermentation is complete. (It takes 17-19 days for the temperature to be below 15, and the production needs to be fermented in a place at a high temperature for 24 hours, and the EM bacteria must meet the following requirements before they can be used

Its color is brown or yellowish-brown; has a strong sweet or sour taste; Pour a small amount into the mineral water bottle with a graduated cylinder, tighten the cap and shake it a few times to produce a large amount of foam; Test pH below.