What is the composition of E. coli s RNA polymerase and what are the roles of the individual subunit

Bacteria, eukaryotes.

and RNA polymerases for bacteriophages.

They differ, but all contain subunits such as , , and δRNA polymerase holoenzyme form is 2' with a total of 5 subunits. where the subunit is associated with the formation of the tetrameric core of RNA polymerase (2'; The subunit contains binding sites for nucleoside triphosphates; 'The subunit contains a binding site to the DNA template; The δ factor is only related to the initiation of RNA transcription, not the extension of the strand, once the transcription begins, the δ factor is released, and the extension of the strand is determined by the tetrameric core enzyme (core

enzyme). Therefore, the role of the δ factor is to recognize the start of transcription and bind the RNA polymerase to the promoter. Place.

E. coli RNA polymerase is 2'Hexamer, 2 of them'Constitutes the core enzyme.

The presence of a subunit was detected on RNA polymerase using urea-polyacrylamide gel electrophoresis with a molecular weight of 10 kDa. However, the subunit is not necessary for the viability of the cell and is therefore a non-essential subunit, although it may play a role in the assembly of the enzyme.

RNA polymerase is an enzyme that synthesizes RNA by polymerizing a DNA strand or RNA as a template, ribonucleoside triphosphate as a substrate, and through phosphodiester bonds. Because it is involved in the transcription of genetic information from gene DNA within cells, it is also known as a transcriptase.

The picture above is a simple illustration of an RNA polymerase, and the yellow round object is the polymerase. Before RNA polymerase, we need to know that transcription requires a double-stranded DNA template, and when transcription occurs, a structure called a "transcription bubble" is created on the double-stranded DNA.

It is evident that there are two regions of single-stranded DNA in the transcription vesicles.

Just like DNA polymerase, RNA polymerase also requires nucleotides, but it requires four ribonucleotides. That is, ribonucleotides are used in the transcription process, not deoxyribonucleotides.

This is ribonucleotide.

Answer]: Wrong [test center] The composition of the whole enzyme. The structural cleavage of E. coli RNA polymerase is composed of five subunits, which are two strands, one strand, and one strom'chain and a factor chain, 2'The four subunits make up the core of the enzyme, which becomes holoenzyme 2 with the addition of factors'

The prokaryotic DNA-directed RNA polymerase is composed of several sensitive branch subunits, and its core enzyme is composed of:

a.α2ββ'

b.α2β'σ

c.α2β'Sue Na Huai

d.ασe.αβ2σ

Correct Answer: a

RNA polymerase holoenzyme form is 2' with a total of 5 subunits.

The subunit is associated with the formation of the tetrameric core of RNA polymerase (2';

The subunit contains binding sites for nucleoside triphosphates;

'The subunit contains a binding site to the DNA template;

Factors are only associated with the initiation of RNA transcription, not the extension of the strand, which is catalyzed by the tetrameric core enzyme once transcription begins, δ factor is released. Therefore, the role of the δ factor is to recognize the start of transcription and bind the RNA polymerase to the promoter site.

RNA polymerase: An enzyme that catalyzes the synthesis of RNA from nucleoside-5-triphosphate using a strand or RNA as a template. It is an enzyme that catalyzes the synthesis of RNA using DNA as a template, ribonucleoside triphosphate as a substrate, and polymerization through phosphodiester bonds.

Because it is related to the transcription of genetic information of gene DNA into RNA in cells, it is also called transcriptase.