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Anonymous users2024-02-08The main factor for the stability of protein solutions is the hydration film on the surface of protein molecules.
Proteins are polymer compounds.
Colloidal in water. Proteins are macromolecules with a hydration membrane and isoelectric, so they can form stable colloids in water.
The stability of the colloidal system of protein solution depends on the following two basic factors: first, the formation of a hydration layer on the surface of the protein; The second is that the surface of the protein has an isotropic charge.
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Anonymous users2024-02-07The stability of the colloidal system of protein solutions depends on two fundamental factors:
1. The hydration layer is formed on the surface of the protein because the surface of the protein particles carries many NH3+; , a COO-, an OH, a SH, a CO-NH, peptide bonds and other hydrophilic polar groups, so it is easy to hydration, and then form a thick hydration layer on the surface of protein particles.
The presence of a hydration layer separates the protein particles from each other, so that the protein particles do not aggregate and precipitate. 0 3 0 5 g per 1 g of protein binding water.
2. The surface of the protein has the same charge In addition to the net charge of the molecule at the isoelectric point, the protein particles have the same charge in the non-isoelectric point state, that is, it has a positive charge in the acidic solution and a negative charge in the alkaline solution, and forms a stable electric double layer with the surrounding counterions. The homogeneous charges of the electric double layer on the surface of protein colloids repel each other, which in turn prevents them from accumulating and precipitating.
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Anonymous users2024-02-06The stabilizing factors of the protein colloidal solution are: the formation of a hydration layer on the protein surface and the homogeneous charge on the protein surface. According to the principle of protein colloidal stability, the gravitational attraction between protein molecules can increase the aggregation and precipitation by destroying these two main stabilizing factors.
Since there are many hydrophilic groups on the surface of the protein, it attracts a lot of water molecules.
On the other hand, protein molecules often have the same charge and exclude each other in a certain pH solution, so the chain protein solution is a stable hydrocolloid.
Methods of destruction
According to the principle of protein colloidal stability, the gravitational force between protein molecules can increase the aggregation and precipitation by destroying these two main stabilizing factors. Such as salting out method, organic solvent precipitation method.
If the appropriate reagent is added, the protein molecule is at the isoelectric point.
state or loss of hydration layer, the colloidal solution of the protein is no longer stable and will precipitate. Protein solutions can precipitate proteins due to the addition of the following reagents.
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Anonymous users2024-02-05There are three factors that maintain the colloidal stability of proteins: the size of the protein molecule, the nature of the charge carried by the protein, and hydration. Proteins are similar to hydrocolloids in aqueous solutions, and their molecular surfaces form a hydration layer and an electric double layer, thus forming stable colloidal particles.
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Anonymous users2024-02-04The two factors that maintain the stability of protein colloidal solutions are the hydration membrane structure and the isocharge.
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Anonymous users2024-02-031.Hydration between protein molecules, water molecules, and molecules.
2.Electrostatic interactions between protein molecules.
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Anonymous users2024-02-02The factors that stabilize the protein solution are the hydration membrane and the same charge.
Hydration film: Most of the surface of protein particles are hydrophilic groups, which can attract water molecules and form a hydration film on the surface of the particles, thereby blocking the mutual aggregation of protein particles and preventing the precipitation of proteins in solution.
Isocharge: In a solution with a pH ≠ pi, proteins carry an isocharge. If pH Pi, the protein is negatively charged; If pH PL, the protein is positively charged.
The factor that maintains the stability of the protein solution is the hydrophilic group of the protein particle, which attracts water molecules to form a hydration film, blocks the protein particles from accumulating and sinking each other, and prevents the precipitation of proteins in the solution.
Protein solutions are an emerging laboratory tool, and they are used in academic research as well as in the production of large-scale pharmaceuticals. The stability of protein solutions is an indispensable and important variable in the process of clinical drug use and biological activity research.
In conclusion, the stability of protein solutions is affected not only by factors such as pH, temperature, light, oxygen content, solvent type, antioxidants, and added salts, but also by their interactions. Therefore, adjusting the above factors can effectively improve the stability of protein solutions.
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Anonymous users2024-02-01a.Brownian motion.
b.Tyndall phenomenon.
c.Protein solution viscosity is large old beam.
d.The surface of the protein particles has a hydration film and the same kind of charge.
Correct answer: d
There is no direct relationship! Look at the structure and arrangement of amino acids! Each protein has a representative amino acid!
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