Chemistry. Pharmacy. High performance liquid chromatograph.

The problem of dilution, how many times diluted is very easy to understand, such as 1ml solution to 50ml, that is diluted 50 times, and then for example, 5ml to 50ml, that is diluted 10 times, the specific complete words can be calculated in this way, dilution times = diluted volume Pipette the volume of the original solution; or dilution factor = original solution concentration Diluted solution concentration. Then talk about the problem of chromatographic data processing, generally you can print out the obtained spectrum, enter the data into excel** for processing, and you can also use the chromatographic work to process the data processing function that comes with it, but this needs to input data such as the concentration of relevant reference substances, and also need to set the relevant parameters of data processing, such as whether you are an internal standard or an external standard.

Dilute 25 times, that is, take 2ml of the original solution and put it in a 50ml volumetric flask, and set the volume. That's a 25-fold dilution.

Liquid phase data processing is generally the external standard method and the internal standard method.

The multiple of solution dilution is simply how many times the volume is enlarged, the volume dilution factor is the diluted volume divided by the undiluted volume is the dilution multiple, high-performance liquid chromatography is only used to see that the detected substance has several compounds in it, or to detect the content and purity of some drugs, and the result chart can be used as a basis for publication.

Diluting 100 times is to take 1ml and a fixed volume of 100ml. Don't forget the dilution factor when processing the data.

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This is more complicated, you have to find a teacher to teach you once, so if you can't say it clearly, the latter will go to the little wood worm to give an instruction manual.

Chromatography is a separation technique in which the mixture of a sample is separated by a continuous distribution of the components of the sample between two phases in a column called a chromatographic separation column.

One of the phases is fixed and is called the stationary phase;

The other phase is a fluid (gas or liquid) that carries a specimen mixture through this stationary phase, called the mobile phase.

As the mixed stream carried in the mobile phase passes through the stationary phase, it interacts with the stationary phase. Due to the differences in the properties and structure of each component in the mixture, and the difference in the magnitude and strength of the force generated between the stationary phases, with the movement of the mobile phase, the mixture is repeatedly distributed and balanced between the two phases, so that the components are retained by the stationary phase for different times, so that they flow out of the stationary phase in a certain order. Combined with appropriate post-column detection methods, the separation and detection of components in the mixture can be achieved.

Classified by two-phase state.

Chromatography in which the gas is a mobile phase is called gas chromatography (GC) g-s & g-l