What to do if the hyphae appear thick during culture

Thicker hyphae indicate that bacteria are growing vigorously in the area, as long as there are no mixed bacteria.

Blank long bacteria generally have the following reasons:

Inadequate sterilization in the early stage: insufficient sterilization time and low pressure of the sterilization pot lead to incomplete sterilization of utensils.

The operation process is not in place: the operation process is not strictly carried out in strict accordance with the sterility, and whether the sample weighing, liquid pipetting, and media pouring are all sterile.

The incubation process is not in place: whether the incubator is cleaned regularly, and whether there are other miscellaneous bacteria contaminating the specific blank and long bacteria need to be investigated one by one according to their own experimental procedures, and then corresponding improvement measures should be carried out, such as extending the sterilization time, repairing the pressure cooker, standardizing the operation steps, cleaning the incubator, etc.

The mycelium does not grow early on the inclined medium, probably because of the low moisture content on the inclined medium.

Inclined medium is a commonly used microbial culture method, which has the advantages of saving medium, easy to operate, and easy to observe. However, microorganisms growing on inclined media need to crawl on the surface of the medium, which requires a high moisture content. In the case of insufficient water content, the nutrient absorption and metabolism of microorganisms will be affected, resulting in slow growth or stagnation.

Therefore, if the moisture content on the inclined medium is too low, the mycelium will stop growing early.

To solve this problem, the moisture content of the inclined medium can be adjusted to a level suitable for microbial growth prior to incubation. In addition, the inclination angle of the inclined medium can be adjusted to a smaller angle to increase the surface bonding of mycelial growth, thereby improving its water absorption capacity.

In conclusion, it is of great significance to understand the basic principles and operation skills of microbial culture for the correct cultivation of microorganisms. At the same time, for different types of microorganisms, it is necessary to select appropriate culture methods and culture conditions according to their growth characteristics and nutritional requirements to ensure their normal growth and reproduction.

Why do some mycelium grow faster and some don't grow in the same material?

First, environmental factors: due to improper cultivation management, such as high temperature in the mushroom room, poor ventilation, and high CO2 concentration, it is not conducive to the differentiation of fruiting bodies, causing mycelial growth; Second, the landslide is a nutritional factor: the nitrogen content in the culture material is high, and the mycelium undergoes a lot of vegetative growth, and the mushroom cannot be twisted.

The third is the species factor: the mother species used in the production of strains belongs to the aerial hyphae, and in the process of transferring the tube, some vigorous aerial hyphae are selected and transferred to the culture material with excessive decay and water content of more than 60%, and when the culture temperature is too high (more than 22), the mycelium is often densely covered with the upper part of the bottle letter hole, and even forms a lumpy. With this strain, it is easy to have mycelium.

After receiving the fungus, the pleurotus eryngii cultivation bag enters the mycelial culture stage, also known as the germ culture. At this stage, it should be dry and moisture-proof, protected from light, ventilated, and insulated from wild sources. The relative humidity of the air in the culture room should be controlled below 70%, and the cultivation bag must not be placed in a humid, unventilated, humidity environment exceeding 80% to 90%, otherwise it is easy to cause miscellaneous bacterial infections and insect pests and cause losses.

Avoiding light and making the cultivation bag germinate under dark conditions is not only a physiological requirement for the growth of mycelium, but also one of the effective measures to prevent insect pests from entering the culture room. Good ventilation is an important condition for the growth of Pleurotus eryngii mycelium. Although the mycelium of Pleurotus eryngii can tolerate higher concentrations of carbon dioxide, fresher air is still beneficial for mycelial development.

Temperature is an important condition for the growth of mycelium, and if the temperature is too high, the mycelium is easy to grow and be weak. If the temperature is too low, it will affect the growth rate of mycelium, and the mycelium will produce mushrooms before the bag is full, which will affect the yield and quality. The germination temperature should be controlled between 22 and 27. 7 In September, the temperature is high, and you can cool down through shade.

No or little ventilation during the day and large ventilation at night. After October, the greenhouse needs to lift the curtain to raise the temperature, and the ventilation time can be arranged during the day. When lifting the straw curtain, black plastic cloth should be used to shade the light in the shed.

In short, it is necessary to flexibly control the temperature, wetness, ventilation and other conditions, and never take care of one or the other.

The problems that should be paid attention to in the management of the mycelium culture of Ferulda mushroom refer to Pleurotus eryngii.

Filamentous colonies are fungi, which are generally cultured in solid medium, and the method of punching is used to knock out the fungus moss from the old colonies with a punch, and then transfer to the new solid medium.

In the process of fungus growth, it starts to grow from the moss and grows outward, so the hyphae closer to the outer edge of the medium are the "younger" and the more "old" the middle is in the middle.

If this filamentous colony produces spores, the hyphae are first vegetative hyphae, which have two parts, divided into intrabasal hyphae and aerial hyphae, the intrabasal hyphae are used to absorb nutrients in the medium, and the aerial hyphae are used for respiration. Later, when the hyphae grow to a certain stage or there is no nutrient in the medium, the hyphae begin to produce powdery spores.

If this filamentous colony does not produce spores, it will continue to grow and the hyphae on the outer edge of the medium will "grow old".

If the fungus produces pigment, it will produce more and more pigment over time, and the color of the colony will gradually darken.

Most of the filamentous colonies are mold colonies, and the colonies of molds can be seen in daily life, and you can see the peels and rotten fruits grown by molds. Actinomycetes can also form similar colonies.

Filamentous colonies. It is a colony of a multicellular fungus and consists of many mycelium. Most of the hyphae are divided into multiple cells and are called septal hyphae, and some hyphae are not septated, which is called non-septal hyphae. Part of the hyphae extends into the medium to absorb nutrients and water, which is called vegetative hyphae; The other part of the hyphae grows into space called the air hyphae, and the air hyphae that can produce spores are called reproductive hyphae.

The shape of the hyphae in the air of some fungi is special, which is racket-shaped, spiral-shaped, antler-shaped, etc., which is one of the bases for the identification of various **filamentous bacteria. Filamentous colonies are cotton-wool, pom-pons, powder, or gypsum powder-like, and can be displayed with different pigments on the bottom and back.