The problem of producing Dendrobium officinale tissue culture seedlings, how to plant Dendrobium off

In fact, the potential of dendrobium in the market is very large, but generally domestic production is sold abroad, maple bucket (that is, air-dried dendrobium) at least 3,000 US dollars a catty.

For you to start a business, the main thing is to have a target market for promotion, I also learn tissue culture, tissue culture seedlings, need someone to buy and plant to be considered successful.

As for the first and investigation, I personally think that the best way is to start a demonstration household in a place suitable for planting dendrobium (generally in the countryside), and the demonstration household will have a good effect and income after planting, so as to drive more people to plant, so that there will be a sales market.

The general growers, all of whom are farmers, dare not invest blindly, so they should have demonstrations, or contact village committees, townships and other institutions to provide certain preferential policies, so that it is easier to promote. ）

The prospect is good, but the production of Dendrobium officinale tissue culture seedlings is not easy to do well without more than 3 years of experience accumulation. Now the best is that many people companies can make seedlings, but the quality of seedlings is different, and the performance of planting is very different.

I am a farmer of Dendrobium, as long as you can make good seedlings, the market should be able to. Now there are a lot of tissue cultures, but there are not many good seedlings, about the market to investigate hemp, you can go to Yunnan to see, the climate here is suitable, and there are more people planting. Sales can go to Zhejiang and Guangdong to see.

I'm going to try to plant a little tin this year, and the seedlings are selling well this year, but I can't say whether they're good or bad! Especially when some people hear that you are going to see the base, they shirk it, and it is estimated that there are a lot!

Joint planting, you engage in seedlings and I plant (imitation wild planting), I have 1492 acres of mountain forest, 1800-2300 meters above sea level, humid climate, suitable for Dendrobium species.

Hello, brother! Allow me to greet you in the usual Yunnan tone. I am from Yunnan and studying in Ningbo, Zhejiang.

After graduating from university, I am going back to my hometown to establish a Dendrobium planting base, so I am very interested in your problem. Also, I know a little bit about the dendrobium market in Yunnan, so maybe we can find some common ground. Intentionally added.

Hello, I am from Xianju, Zhejiang, I planted 20 acres, and I will plant 30 acres as soon as everything is ready, and I need good acres. We've just started here, and we're already hundreds of acres in size.

I am from Taizhou, Zhejiang, and currently have 200 acres of mountainous land in Guangxi, planted in the open air, which is convenient to add a q543441776****.

Have you done a good job of tissue culture?

In summer, direct sunlight should be avoided and shaded should be provided. Place on the south balcony in spring, autumn and winter. All are controlled at about 28 degrees.

It is better to control the humidity at about 70%. As for the moisture, it seems that it is advisable to water the iron sheet once every two or four days in summer, and once every 15 days in other seasons. Overall watering, the principle is easy to dry and not easy to get too wet.

In this way, the growth period of Dendrobium officinale will be higher than five years.

Propagation methods are commonly used for propagation by dividing, cuttings and tissue culture.

1. Dividing propagation is carried out in spring combined with pot change. The densely growing mother plant is lifted out of the pot, with less damage to the roots and leaves, and the orchid seedlings are gently broken, and 3 to 4 plants are selected to plant 15 cm pots, which is conducive to forming and flowering.

2. Cuttings propagation select non-flowering and full-growing pseudobulbs, cut them from the rhizosphere, and then cut them into a section of every 2 to 3 sections, directly insert them into peat moss or wrap the base of the cuttings with water moss, keep them moist, room temperature at 18 to 22, and take root 30 to 40 days after cutting. Wait for the roots to grow 3 to 5 cm long in pots.

3. Tissue culture propagation often uses shoot tips and leaf tips as explants, and the differentiation rate can reach about 1:10 on the MS medium with an additional 2,4 mg L and 6 mg L of benzylaminoadenine.

Summary. Potassium had the greatest effect on the number of new roots and relative weight gain, while phosphorus had the greatest effect on the number of leaves. The results of analysis of variance showed that nitrogen, phosphorus and potassium had no significant effect on the number of leaves and the number of new roots in Dendrobium officinale tissue culture seedlings (p ).

Potassium had the greatest effect on the number of new roots and relative weight gain, and phosphorus had the greatest effect on the number of leaf sedans. The results of analysis of variance showed that nitrogen, phosphorus and potassium had no significant effect on the number of leaves and the number of new roots in Dendrobium officinale tissue culture seedlings (p ).

What is the effect of low pH on tissue culture seedlings.

pH is the pH of the material, too high or too low, will cause poor growth of plant tissue, and the pH value, too high or too low, will cause Jingsun medium (agar or carrageenan) to be too hard or too soft (can not cover the hidden condensation), will affect the growth of plants.

There are two production methods for Dendrobium officinale tissue culture seedlings, one of which is to use seeds.

One of the tissue culture techniques of Dendrobium officinale is to directly use the stem segment of Dendrobium officinale to cultivate, and the induction rate of callus by different explants is very different, and the callus healing rate induced by stem segment is the highest, which is achieved. Obtain a large number of Dendrobium calli and seedlings. The other is to use Dendrobium officinale seeds, after the seeds germinate to form protocorms, use the medium to induce and proliferate Dendrobium officinale to obtain a large number of Dendrobium protocorms, and then cultivate Dendrobium seedlings.

The quality of tissue culture seedlings produced from seeds was significantly higher, which was conducive to the production of Dendrobium officinale tissue culture seedlings.

The researchers isolated 25 endophytic fungi from wild Dendrobium officinalis, of which 5 fungi could promote seed germination, 7 fungi could form symbiotic relationships with Dendrobium officinale seedlings, and 3 fungi could promote the growth of seedlings. Therefore, the isolation and screening of suitable mycorrhizal fungi that promote the growth and development of Dendrobium officinale is one of the key problems to improve the survival rate of test-tube seedlings transplanting and solve the production problems.

The polysaccharide content of Dendrobium officinale tissue culture seedlings and wild products is basically the same. The comparative study of plant morphology, stems, leaves and other tissue structures and the comparison of total polysaccharide content showed that the plant morphology and tissue structure of Dendrobium officinale were similar to those of wild products, and there was no significant difference in the content of total polysaccharides of the main components, and the quality of the two medicinal materials was similar. Therefore, Dendrobium officinale seedlings can replace wild medicinal materials after field cultivation, and a large number of test-tube seedlings can be cultivated through tissue culture, and the development of artificial cultivation of Dendrobium officinale is an effective way to solve the problem of shortage of Dendrobium officinale resources.

Dendrobium officinale tissue culture is to use the seeds of Dendrobium officinale to carry out tissue culture, so that the survival rate can be greatly improved, and the seedlings that are picked up by seeds generally do not exceed ten generations need to be eliminated, so they need to be updated every year, and the main method is to take the seeds of wild Dendrobium officinale.

Don't believe it, it's all manipulated by some ** companies. There is no market for Dendrobium officinale at all.

The first floor is also a bit excessive, and now the dendrobium is indeed touted too high by some people with ulterior motives. But if there is no market at all, will anyone tout the industry? Our company's tissue culture seedlings are made of seeds, and the number of embryos in Dendrobium officinale seeds is very large, which is very convenient to use, and there is no need to use explants such as rhizomes.

Tissue culture was performed using the seeds in the capsule.

To use the fruit of dendrobium can be contacted if necessary.

Dendrobium officinale is a herbaceous plant that grows on semi-shady rocks at an altitude of up to 1,600 meters, and prefers a humid, warm, half-shade and half-yang environment. Dendrobium officinale is not cold-tolerant, and can generally only resist low temperatures of -5, and is mostly distributed in Huoshan, Anhui, Zhejiang, Guangxi, Fujian, Yunnan and other places.

The temperature is moderate. Planting Dendrobium officinale generally requires an indoor temperature of 0-30 and a growth temperature of 15-30. In summer, indoor injection or Hu Yeyi shade and ventilation, often watering or spraying to moisturize and cool down; In winter, you should pay attention to the temperature in the room and keep the plants warm.

Proper humidity. The planting requirements of Dendrobium officinale are moderate and appropriate, and the adjustment of humidity changes according to the level of temperature, high temperature requires high humidity, and low temperature requires low humidity. During the vigorous growth period, the indoor air humidity should be kept at 60%-90%.

The water quality is moderate. When cultivating Dendrobium officinalis, it is required to use weak acidic water or neutral water, and the substrate used in cultivation is required to be easily breathable, easy to leak, and have many voids.

The venue is suitable. The cultivation site of Dendrobium officinale requires good ventilation facilities. The room needs to be warm, moist, low light, and ventilated.

First of all, it is necessary to choose raw materials according to local conditions. There are many materials used for fermentation substrate, such as garden leaves, dead branches, straw, sawdust, bark, shavings and other industrial and agricultural wastes and scraps. Users can choose cheap and easily available materials according to local conditions, and landscaping companies with good conditions can also choose one or more scientific combinations of materials for fermentation.

And then there's the operation:

1. Prepare nutrient solution. Urea water is made by mixing kilograms of urea with 150-200 kilograms of water, and the water content of the fermentation material is controlled at 60-65%. This process is to prepare microorganisms"Nitrogen"Nutrient solution (required for the reproduction of microorganisms themselves"Nitrogen"、"Carbon"and other nutrients, urea contains"Nitrogen"high).

The method of judging whether the moisture is suitable or not: grasp a handful of materials with your hands, see the watermark but not drip, and disperse when you land. Less water, slow fermentation, more water, poor aeration, and more, will also lead"Spoilage bacteria"Work produces a foul smell, remember.

2. Material stacking. The general requirements for the construction of the fermentation pile of the materials with the adjusted moisture: the height of the pile is meters (remember not less than 80 cm), the width is 2 meters, the length of the pile can be adjusted freely according to the amount of fermentation material, the general length is not less than 2 meters, and the total volume of a single fermentation pile is not less than 3 cubic meters.

3. Sprinkle inoculation. Wait until the urea water is soaked, cover it with breathable covering, leave it naturally for 24 hours, and mix 2 kg of Gymboree microbial fermentation agent with 5 kg of rice bran to form an incremental fermentation agent (that is, 2 kg of hair starter culture"Dilution"into 7 kg of material, more things are easy to sprinkle, but also to provide nutrients for the strain), evenly sprinkled in the pile of materials that have been piled up, so that while turning, while sprinkling, this process is called"Vaccination"(That is, the Gymboree microbial fermentation agent is mixed with the above other materials and sprinkled into the pile, which is technically called microbial engineering.)"Vaccination"）。Note:

Rice bran is not a uniform bran, and the nutrition and aeration of fresh rice bran are much better than those of old rice bran. In case there is no rice bran, you can use bran, corn flour, etc. instead).

4. Cover and overturn. After receiving the seeds, cover the large pile with breathable covering, which should be shaded and protected from rain. Under normal circumstances, after the fermentation process begins, after 5-10 days of fermentation, the temperature can reach more than 55-60, at this time, turn once, and accumulate 2-3 times to complete the fermentation.

5. Fermentation is completed Under normal circumstances, the fermentation time of straw and deciduous substrate is about 15 days, sawdust substrate is 25-30 days, and bark can be fermented in 30-40 days. The fermented material is black-brown, soft to the touch, fragile, and can be used when cool-dried.

It is best to use bark, other substrates are not easy to breathe, and root rot is easy to occur.

If the pod is not cracked, only the surface of the pod needs to be disinfected. Rinse under running water Soak the laundry detergent for 30min and rinse well.

Ultra-clean Taizhong) 75 alcohol disinfection 30s Sterile water rinse Mercury disinfection 5 8min Sterile water rinse 5 times Sterile absorbent paper is blotted dry, and the sterilized scalpel breaks the capsule and sows directly. (The pod is an enclosed environment, and the seeds are sterile when they are not dehisced).

If it is a dehisced or previously preserved seed. Wrap the seeds in dense gauze and absorb them with sterile water.

Pretreatment in mol lnaoh for 10min Rinse with sterile water, put it in 5 sodium hypochlorite for 5 10min Rinse with sterile water several times, and blot dry with sterile absorbent paper for inoculation.

Seed treatment for uncracked capsules is simple, and any of the following methods will work:

1.On a clean bench, the fresh capsule is treated with 75% alcohol for 30-60 seconds, and the sterile stainless steel dish is removed to allow the alcohol on the capsule to air dry naturally, and then the capsule is cut open with a sterile scalpel and the seeds are inoculated into the culture medium for culture. The success rate of this method can reach more than 80%.

2.Soak fresh mercury in mercury solution for 6-10 minutes, drain the solution, rinse with sterile water for 5 times, and then inoculate and culture according to the method of "1". The success rate is above 90%. This method is the most cumbersome.

3.Soak fresh capsules in 1000 mg l of chlorine dioxide solution for 20-30 minutes, drain the solution, and inoculate the culture according to the method of "1". The success rate is above 95%. This method is the easiest and quickest.

There are other ways you can try to do it, but it is recommended that you don't complicate the program.