Chlorella culture method, the simplest medium formulation for chlorella

Today's farmers basically know that the fungus is expanded and then used, one is to save costs, and the other is to increase the use of the strain after the expansion of the effect is better. In fact, algae in aquaculture is more important than the bacterial phase, so why not expand the algae species and use them? But how to expand algae in Tangkou.

After all, it is more professional, but it is rarely studied. The author has been engaged in algae production and cultivation for many years, and this year I have taught several farmers in Jiangsu and Tangkou, Zhejiang Province to expand the cultivation of chlorella.

The effect is good, and I will share it with you, I hope it will be helpful to the majority of farmers.

First of all, let's popularize the basic knowledge of algae: this is the algae culture diagram in the laboratory state, and the conditions required for the rapid growth of algae: light, aeration, and culture medium.

Temperature. The most important point is to inoculate and grow in a sterile state, and if you are not careful, you will fail as soon as you are contaminated, which is also the main reason why laymen can't do it. I think that when we entered the first-level culture room, we disinfected with alcohol every day, and our palms were red.

This is a common chlorella species on the market, and if it is sprinkled directly into the pond, no matter how high the concentration is, it cannot withstand the loss in its natural state: shellfish should be filtered, and silver carp should be flowered.

To eat, there are countless unseen protozoa.

Waiting with a "big mouth", coupled with the fact that the weather is not good, the light is poor and the temperature is low, the combined impact will be a failure.

What I'm going to teach you now is to turn that little bucket of chlorella seeds into a big bucket to use.

This involves a problem of inoculation ratio, if you want it to grow fast, the color of the inoculation will be dark, and the reverse will be light. Our farmers rarely know how to look at the microscope, and the chlorella they bought is not authentic or not, in fact, it is very simple to verify, so that if it grows for five to seven days, the color is always that green, it is not an algae species, it may be dyed. The real algae species will multiply quickly, the concentration is very large in about 3 days, the wall of the barrel will be attached to the wall or the bottom of the algae residue precipitation, if the expansion fails, often a bucket of water is crystal clear.

I'm in Zhijiang, Hubei.

I met a farmer who often used chlorella to grow fertilizer water and ponds.

It is green all year round, and the water color is beautiful, but the silver carp does not grow, and this is the problem of algae seeds.

What should I pay attention to during the cultivation process?

The vertical culture method is more cost-effective, and an iron cage and a piece of plastic sheeting are sufficient.

Chlorella can use light energy and carbon dioxide for normal autotrophic growth under autotrophic conditions, and can also use organic carbon sources for growth and reproduction under heterotrophic culture conditions.

At present, the cultivation methods mainly include open culture and closed culture. Open culture is carried out in open containers, generally with cement pools, and CO2 can be supplemented by artificial supply or by relying on natural exchange with air, and by manual stirring to dissolve the CO2 in the air. But there are some drawbacks to open culture that are not easy to overcome:

First of all, it is inevitably affected and limited by natural conditions (such as sunlight, temperature, etc.), and it is difficult to maintain stable output and quality. Secondly, it is susceptible to contamination and even feeding by xenomorphic organisms, which causes the degradation and even culture failure of the cultured algae. The closed culture mainly includes closed fermentation tank and glass pipeline photosynthetic bioreactor culture, centrifugal water pump stirring or airlift stirring, and supplementation of inorganic nutrient solution or organic nutrient solution and CO2, and the yield is about 10 times that of open culture. The process flow of large-scale cultivation of chlorella with a pipeline photosynthetic reactor is shown in the figure.

Although the cost of closed culture equipment is high, it is easy to control, can resist pollution, can improve yield, and the stability of the biological system is good, and the quality and yield are relatively stable. Chlorella culture can use both natural light and artificial light, the optimal light intensity is 2000 5000 lux, the temperature is 25 30, PH615 715, mainly using inorganic nutrient solution for culture, can also add fructose, acetic acid, glycerol, galactose, glucose and other substances to the culture medium, so that it becomes heterotrophic growth. The formula of nutrient solution varies according to the purpose of the product, as a product for the purpose of human health food and pharmaceutical products, the inorganic nutrient solution requires the preparation of analytically pure chemical reagents; As a feed additive product, chemical fertilizers can be used to supplement trace elements.

Chlorella is thinner than other microorganisms, so effective dehydration steps are required during harvesting. In the past, coagulation, flocculation, precipitation, filtration, etc. were often used to harvest chlorella cells, but these methods seriously affected the quality of the product. This method is effective, but requires high investment and energy consumption.

When cultivating chlorella, it is necessary to first prepare a culture medium, with a concentration of 10 mg l of nitrogen and 4 mg l of phosphorus, and then take an appropriate amount of tap water, dissolve it and shake it well. And buy algae seeds that have not been polluted and are in good condition, put them in the culture medium, the inoculation amount is about 1 10, and then put the lid on and put it under the sun, the temperature is kept at about 25, and it is OK to shake it twice a day.

1. Clean the culture container or site;

2. Connect half a ton of water source suitable for cultivation, tap water can be prepared one day in advance, add a bottle of nutrient salt, and stir well;

3 Add 2 bottles of super-concentrated algae, about 72 hours after the algae grow, then add half a ton of water, and add 1 bottle of nutrients;

The algae can be harvested after 3 days.

Precautions: 1. Keep the container or site clean, and choose a sunny day to inoculate and expand in the morning;

2. Use a thermometer to measure the water temperature twice a day, and the temperature should be heated or shaded in time when the temperature is lower than 20 degrees or higher than 30 degrees;

3. Ensure that the site has proper light, the most suitable light for chlorella is 5000-10000lx, if it is a large hatchery site, choose a pool on the side;

4. Add oxygenation or stirring, the density of super-concentration is large, the purpose is to make the algae float up (the temperature is higher than 25 degrees, the light conditions are excellent, oxygenation can be set up, and stirring 2 times a day can be done);

5. Due to the different conditions, temperature, light and water conditions, it is recommended that the initial inoculation concentration should not be too low to ensure the success rate.

6. Supplement the nutrient salt, which can be fully dissolved in a small bucket and then sprinkled.

1. Autotrophic culture: The autotrophic culture of chlorella can use both natural light and artificial light, and the medium is mainly composed of inorganic compounds, with an optimal pH value, an optimal light intensity of 36 90 mo l (m2·s), and a temperature of 20 30. Studies have shown that pH value is an important factor affecting the growth of chlorella, and light intensity and ventilation regulate the growth of chlorella by affecting the photosynthesis intensity of chlorella.

At present, the cultivation methods of photoautotrophic energy include open pool culture and closed culture. The equipment is simple, the technology content is low, and the investment is low, but the output is low and it is easily affected by natural conditions.

2. Closed Peiran: There are two main ways to cultivate in closed fermentation tanks and glass pipeline photosynthetic bioreactors. Compared with the former, the nutrient content of chlorella after heterotrophic culture was reduced, but the harvest per unit volume of heterotrophic algae was 10 times higher than that of autotrophic algae at the same culture time.

Therefore, the single-cell protein yield per unit volume of chlorella per unit volume under heterotrophic conditions was still higher than that of autotrophic chlorella.

In this regard, it can be cultivated directly in the nutrition base, relatively speaking, there is no problem, and under normal circumstances, it is enough to control the temperature and humidity to ensure sufficient fertility.

The cultivation method of chlorella, this is what various merchants will tell you when you buy it, or you can find a straight man on the Internet.

The chlorella culture method is a kind of um-cleansing of the body. He is one of the more advanced modern ones for recuperation. A healthy treatment for the body.

The method of drifting, the main thing is to make the ball the main um, the ball can be cultivated directly earlier, and it is very good to have such a thing.

Its cultivation method is also suitable for the kind of cultivation that must be cultivated according to the regular method, so that he will be healthier.

Cooperative preparation method, you should check some professional information or something, learn more.

In the case of chlorella, the cultivation method is still very good, and the taste is very good.

If you want to cultivate the nama, don't come in a little bit, put it in the water.

Chlorella should be treated with a nutrient solution and cultured in a well-lit place.

Water quality selection, change the water once a week, use tap water that stands in the sun and completely volatilizes chlorine; Reasonable temperature, the suitable growth temperature is 5 22, with a certain cold resistance, when the temperature exceeds 35, the color becomes dark; It is suitable for light, has a strong resistance to negative, and is placed in a cool place to avoid strong light during maintenance; Proper fertilization, proper application of nutrient solution when changing water, and input of carbon dioxide can lead to more lush growth.

When cultivating chlorella, you need to change the water once a week, and if you want tap water, you need to let it stand in the sun for 2 to 3 days until the chlorine and other substances in the water have completely volatilized and the water quality has changed. The water temperature is controlled below 25, and it likes a cool growing environment, and needs shade in summer, avoiding direct sunlight, which is conducive to growth and verdant in color.

When chlorella is cultivated, if the temperature is high, prepare the cavity to keep it in the refrigerator or put a small amount of ice in the water. The suitable growth temperature is 5 22, with a certain cold resistance, and the poor fiber can survive in cold water, but the growth rate will be slower, not resistant to high temperature, and the color will become dull when the ambient temperature exceeds 35.

Chlorella has a strong resistance to negativity, and needs to be kept in a cool place away from strong sunlight when maintaining. Most of the native areas are located in the lake waves at high latitudes, and there are long periods of ice in winter, and there is no sunlight for a long time. If you put it at home for cultivation, you can plant the surface of a static object in the water tank, or use a spherical culture virtual rolling imitation dish for cultivation to improve ornamentality.

Chlorella is highly adaptable and needs to be fertilized in the process of growth, too much fertilizer will lead to fertilizer damage, and lack of fertilizer will cause malnutrition and slow growth. Proper application of nutrient solution and carbon dioxide during water change can make it grow more lush, accelerate the expansion of the body, make it more rounded and shapely, and improve the ornamentality.

Chlorella vulgaris is a spherical single-celled algae with a diameter of 3-8 microns, one of the earliest life on earth, which appeared more than 2 billion years ago, and is an efficient photosynthetic plant, which grows and reproduces with photosynthetic autotrophic growth and wide distribution.

Chlorella is a unicellular algae, often solitary, but also multicellular aggregates. The cells are spherical, oval, and contain a periphytic, cup-shaped, or flaky pigment body. Vegetative reproduction, each cell can produce 16 quasi-spores, and when the mother cell ruptures, the spores escape, and when they grow up, they become new individuals.

The cells are high in protein, fat, and carbohydrates, and have a variety of vitamins that can be eaten and used as bait.

At present, there are about 10 species of chlorella known in the world, and there are hundreds of varieties of chlorella. Chlorella is widely distributed in nature, with freshwater being the most diverse. It is easy to cultivate, not only can use light energy for autotrophic growth, but also can use organic carbon sources for growth and reproduction under heterotrophic conditions; And the growth and reproduction rate is fast, it is the only organism on the earth that can grow 4 times in 20 hours, so its application value is very high. The common species in China include Proteonuclear Chlorella, Chlorella elliptical, Chlorella vulgaris, etc.

Efficacy of chlorella in aquaculture.

1. In the early stage of breeding, chlorella and fertilizer are used at the same time to play the role of rapid fertilizer and water. Chlorella can be used alone during the high temperature period, or it can be splashed with Bacillus subtilis or EM bacteria at the same time to regulate water quality, reduce ammonia nitrogen and nitrite, inhibit cyanobacteria, and improve the water environment.

2. Provide a source of unicellular chlorella, which can multiply rapidly after entering the aquaculture water body, forming a water body with unicellular chlorella as the dominant population, and building a good living environment for fish, shrimp, crab, shellfish and other aquatic animals.

3. Chlorella can provide rich and balanced natural nutrients, rich in protein, unsaturated fatty acids, vitamins, minerals, chlorophyll, alcopolysaccharides, nucleic acids, etc. These nutrients help improve disease prevention and resistance.

4. Chlorella has high nutritional value, which can be used as open bait for shrimp, crab and shellfish seedlings, and direct feed for filter-feeding fish, promoting growth, reducing costs, and improving the survival rate of aquatic animals.

5. It can better carry out photosynthesis, increase the dissolved oxygen of the water body, and greatly reduce the possibility of hypoxic floating head.

Precautions for chlorella algae sap culture.

1. The best water used is well water or tap water after 3 days of aeration, and the culture water cannot contain bleaching powder. Pond water should never be used.

2. Clean the culture container, and enlarge or shrink it according to the size of the culture container according to the above addition ratio.

3. It is best to inoculate and cultivate at noon on a sunny day to ensure sufficient light and suitable temperature, and have oxygenation equipment.

4. The medium must be added in the order of green solution, colorless solution and yellow solution.

5. In order to avoid exposure to the sun at noon in summer, it is necessary to cover with plastic film or other coverings.

6. If there is a small amount of precipitation, it is normal, and it can be shaken.