How to make a mother seed of a culture? How to make the culture

First, the selection of edible mushroom culture bottles: 100 ml medical saline bottle instead of test tube as a container for holding culture medium. 2. Preparation of medium 1 Recipe: 200 grams of potatoes, 20 grams of glucose, 18 20 grams of agar, 1000 ml of water.

2 Production: Dig out the sprout eyes of the potato, peel and wash, cut it into thin slices and add water, boil it in an aluminum pot for 25 30 minutes, filter it with 4 layers of gauze, take the filtrate and put it in the aluminum pot, add agar, heat it over low heat and stir constantly, add glucose after the agar is dissolved, add 1000 ml of boiling water, stir well, put it into a salt water bottle while it is hot, 8 10 ml per bottle, plug the tampon Tampon is required to be dry, elastic and of appropriate length, and then wrap the tampon and the bottle mouth with kraft paper, and tie the bottle mouth tightly with a rubber ring. 3. Sterilization is autoclaved, if there is no special pressure cooker, you can use a household pressure cooker for sterilization.

The method is to place the culture bottle filled with medium in the pressure cooker, leave a gap between the bottles, and do not stack it too tightly, so as to facilitate steam circulation and sterilize thoroughly. When the pressure gauge pointer points to 0 05, open the air release valve, concentrate on discharging cold air, and when the pressure drops to zero, close the exhaust valve for pressure boost sterilization, and keep it for 30 minutes after automatic deflation Household ordinary pressure cooker sterilization for 1 5 hours, let it cool naturally, take it out and place it in the inoculation box for later use.

Fourth, the isolation of strains mainly adopts the tissue isolation method. Usually from the edible fungus fruiting body to isolate the pure strains, during the operation, cut a small piece of tissue from the fruiting body, put in the medium to grow pure strains, and the mycelium germination is fast, the genetic traits are stable, and the offspring are not easy to mutate. 1 mushroom sterilization:

After the mushrooms are harvested, the stalks are cut off, placed in a washed dish, and irradiated in an inoculation box equipped with a UV lamp for 20 to 30 minutes. 2. Inoculation block cutting: In the sterilized inoculation box, tear open the fruiting body, use a knife that has been sterilized and cooled by an alcohol lamp flame, cut a soybean-sized tissue block inside the junction of the stipe and the cap, and put it into the medium in the strain bottle for culture.

3. Selection site of inoculation block: The selection site of inoculation block varies according to different types of edible fungi, and the tissue block is cut at the junction of the skin and the fold at the outer edge of the cap for separation. For fungi with small bodies, thin caps and empty stalks, such as enoki mushrooms, the caps should be quickly knocked off and the tissue at the growth point at the top of the stipe should be hooked with an inoculation needle for separation.

For umbrella fungi that are not easy to germinate with hollow spores in very thin stipes, the fruiting body with semi-permeated membrane can be selected by the sub-solid layer separation method, and moved into the medium, and the mycelium will be transferred to the new medium after germination. For polypores with large fruiting bodies and firm textures, the outer edge of the fruiting body should be taken from the flesh and tissue blocks. The fruiting body with low water content is cut into small pieces of 5 square centimeters, baked on an alcohol lamp flame for 5 10 seconds to make the fruiting body appear slightly charred, and then a small piece of fungal flesh tissue is cut with a sterile knife and transferred to the culture medium.

5. Culture and managementPut the strain bottle into the tissue block vertically on the indoor shelf or platform, and the indoor temperature is controlled at 20 28, and the tissue block germinates after 1 2 days. Pay attention to observation during hyphal growth. The contamination was found and picked out in time, and after about 10 days of mycelium growing all over the bottle, the mother seed was successfully made.

What are the appearance requirements of the mother seed in bacterial production?

The cultivation methods of mushroom strains include isolation culture, inoculation culture, and liquid culture.

1. Separation culture

This is the first step in the cultivation process by isolating the mushroom species from the parent species, and in general, there are methods such as spore isolation, tissue isolation, and intrabasal hyphae isolation. According to the characteristics of mushrooms, we generally use the multi-spore isolation method: that is, many spores are inoculated on the same medium, and they are cultivated and mated together, and then high-quality edible mushrooms are obtained.

2. Inoculation and culture

We have obtained good quality mother seeds in the above isolated cultures, but in order to select a good and high purity mother seed and make it a usable stock, we also need a culture medium. Therefore, generally speaking, through the inoculation and culture of the bacteria, some mushroom hyphae with strong vitality, mellow color, and robustness are found to be used for inoculation and culture of original seeds and cultivated seeds.

3. Cultivating liquid species

In addition to quality, we should also pay attention to the yield when cultivating mushroom strains and mycelium, so the method of using liquid seeds to obtain strains by deep fermentation can maximize the yield, shorten the entire cultivation and production cycle, and effectively reduce costs.

Of course, because the current stage of technology is not yet mature, so this is still an ideal goal to achieve edible strains, and with the development of science and technology in the future, it is believed that the role of liquid species will be greater play and discovery.

Prepare the ingredients: wood chips, bran, gypsum, sugar and fungicide. Matrix Preparation:

Stir dry culture materials such as wood chips, bran, gypsum, etc., and add sugar and fungicides. Inoculation of mycelium: Cover the surface of the culture with the culture of Kaifeng.

Incubation environment: The bag is placed on the culture rack and the temperature is kept at 25-27.

1. Prepare the ingredients

For the production of strains, first of all, it is necessary to prepare a suitable substrate, which is different from plants, suitable for growing in the soil of wood fiber, and the medium formula is 20% sawdust bran, 1% gypsum, 1% sugar, and fungicide, and the group is prepared after completion.

2. Matrix preparation

Weigh the ingredients according to the proportion of the strain cultivation and nutrient base formula, place the dry culture materials such as sawdust, bran, gypsum on a clean cement floor, stir evenly with a shovel, and then pour cold water into the container according to the ratio of material to water, add sugar and fungicide, and stir until all are dissolved.

3. Inoculation of strains

Use a tough plastic bag to fill the material, tie the bag mouth tightly for sterilization, wait for a period of time and start inoculation, try to cover the surface of the culture material with the bacteria, form a growth advantage, can germinate quickly, and tie the bag mouth tightly after completion, and carry out culture.

4. Cultivate the environment

Mycelial culture is suitable for a closed environment, the culture bag should be placed on the culture rack, the temperature should be kept at about 25-27, and the bag should be turned and disinfected regularly, about 30 days later, the mycelium will be filled with plastic bags, and when its color changes to strong white, it will be removed from the culture room for use.

Here's how to make your own culture:

1. First of all, the branches used to make the fungus should be cut into a small section, and then they should be placed in the disinfection pool and covered with water, and the water must not be over the branches.

After soaking the shoots thoroughly, add a small amount of quicklime until the pH value is alkaline. Then drain the shoots and put them into a polypropylene seed bag, which must be steamed in a pressure cooker for 3-10 hours for sterilization, and then allowed to be inoculated after cooling.

2. When inoculation, it is necessary to pay attention not to use first-class species whose medium has been dried up or aged, so as to avoid affecting the survival rate of strains or leading to the degradation of production traits.

The long-term preservation of the strains must be placed in the incubator before use, and the culture temperature should be increased, and the activation culture time is generally about 1-3 days.

3. If you want to use the first-class species that are preserved, you must check whether there is any pollution.

For example, if there are black, green, yellow and other colonies on the slope of the test tube, it means that the bacteria have been contaminated by mold. If there are yellow-brown dots or irregular patches under the aerial hyphae, it is also an indication that the culture is contaminated with bacteria.

Preservation method of the strain: inoculate the strain on a suitable solid slope medium, wrap the tampon part with oil paper after the strain has fully grown, and then put it in the refrigerator at a temperature of 2-8. 80% glycerol was treated with high-pressure steam for the bacterium dentergentus, and then the bacteria were placed on the slope culture culture base for culture, and a high-concentration bacterial suspension was prepared with sterile water.